낙동강 하구에서 채집한 문절망둑 Acanthogobius flavimanus의 간장 약물대사효소계와 성호르몬 농도
esponses in Hepatic Xenobiotic Metabolizing Enzymes and Sex Hormones of Yellowfin Goby Acanthogobius flavimanus in Nakdong Estuary
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
- To assess effects of contaminants on fish in Nakdong river, feral yellowfin goby Acan-thogobius flavimanuswere caugt in two different sites and its hepatic monooxygenase enzyme,including cytochrome P450 (CYP), NADPH-cytochrome P450 reductase (P450R), NADH-cytoch-rome b5 reductase (b5R), ethoxyresorufin deethylase (EROD), glutathione S-transferase(GST)were quantitatively determined. Gonadosomatic index(GSI), hepatosomatic index (HSI) and thresex steroid hormone (17β-estradiol, E2; testosterone, T; 11-ketotestosterone, 11-KT) levels of thefish were also investigated. HSI of fish from polluted site (site 1) were significantly higher than that of unpolluted site(site2), but GSI levels were significantly lower in polluted site. No significant differences in plasma 11-KT and T levels were observed in two sites surveyed. E2 level was, however, significantly (p0.05) higher in female fish from site 1 than site 2. In addition, hepatic EROD activity and CYPlevel of site 1 fish were lower than those of site 2 fish, whereas relatively high levels of P450R, b5Rand GST activities were found in site 1. The results imply that yellowfin goby, especially female fishin Nakdong river estuary are affected from contaminants disrupting sex steroid hormone system.
참고문헌 (36)
- 곽석남, "낙동강 하구역 어류의 종조성 변화", 한국수산과학회지, 36(2), pp.129~135 (2003), 한국수산과학회
- 김기현, "낙동강 하구역 퇴적물 중금속의 분포와 오염의 역사 추정", 한국해양학회지, 5, pp.285~294 (2000)
- 김성철, "낙동강 퇴적물의 Nonylphenol과 Bisphenol A 오염도 평가", 한국물환경학회지, 19(4), pp.357~366 (2003), 한국물환경학회
- 문성기, "낙동강 중, 하류의 식물플랑크톤 군집구조", 한국환경과학회지, 10, pp.41~45 (2001)
- 정기호, "낙동강 유역에 서식하는 붕어와 황소개구리 체내의 Polychlorinated biphenyls의 축적도와 분포특성", 분석과학, 17(4), pp.347~354 (2004), 한국분석과학회
- 박명희, "문절망둑(Acanthogobius flavimanus)의 생식소 발달과 혈중 성 스테로이드호르몬 농도 변화", 한국수산과학회지, 38(5), pp.309~315 (2005), 한국수산과학회
- 박영규, "낙동강유역 하천수의 농약 유출 특성 : 농경지 및 골프장에서의 농약 유출의 평가", 대한환경공학회지, 18, pp.627~636 (1996)
- 서정관, "낙동강 수계 하.폐수 처리시설의 방류수가 조류 성장 잠재력에 미치는 영향", ALGAE, 18(2), pp.157~167 (2003), 한국조류학회I
- 양성렬, "낙동강 하구역의 담수유입에 따른 해양환경 및 일차생산력 변화", Algae, 16, pp.165~177 (2001)
- 양홍준, "낙동강하구의 어류상과 댐의 어도에서 어류의 이동", 육수지, 34, pp.251~258 (2001)
- 윤명희, "낙동강 하구수의 변이원성에 대한 연구", 한국환경과학회지, 10, pp.35~39 (2001)
- 이순화, "강둑여과수와 낙동강 수질의 변이원성에 의한 평가", 대한환경공학회지, 22, pp.1671~1681 (2000)
- 이지선, "시화호에서 채집한 풀망둑 Acanthogobius hasta의 간장 약물대사효소계 및 항산화계의 반응", 환경생물, 26(2), pp.94~101 (2008), 한국환경생물학회
- 정기호, "낙동강 하류 유역의 저니토, 토양, 잡초 및 채소 중의 중금속의 분포와 상관관계", 한국환경과학회지, 5, pp.801~812 (1996)
- Adams SM, "Application of biomarker of bioindicators in assessing the health of fish populations experiencing contaminant stress, In Biomarkers of Environmental Contamination", pp.333~353 (1990), Lewis Publishers
- Aida K, "A review of plasma hormone changes during ovulation in cyrinid fishes", Aquacult, 74, pp.11~21 (1988)
- Burke MD, "Ethoxyresorufin: direct fluorometric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene", Drug Metab. Disp, 2, pp.583~588 (1974)
- Dey S, "Ovarian damage to Channa punctatus after chronic exposure to low concentrations of elsan, mercury, and ammonia", Ecotoxicol. Environ. Saf, 17, pp.247~257 (1989)
- Goksφyr A, "Accumulation and effects of aromatic and chlorinated hydrocarbons in juvenile Atlantic cod (Gadus morhua) and rainbow trout (Salmo gairdneri)", Toxicol. Appl. Pharmacol, 89, pp.347~360 (1994)
- Habig WH, "Glutatione-Stransferase, the first enzymatic step in mercapturic acid formation", J. Biol. Chem, 249, pp.7130~7139 (1974)
- Karels AE, "Effects of ECF bleached kraft mill effuent on reproductive steroids and liver MFO activity in perch and roach", Proceedings of SETAC-Europe 7th Annual Meeting, pp.257~ (1997)
- Kime DE, "Endocrine Disruption in Fish" (1998), Kluwer Academic Publishers
- Livingstone DR, "Organic xenobiotic metabolism in marine invertebrates, In Advances in Comparative and Environmental Physiology", pp.45~185 (1991), Springer
- Livingstone DR, "The fate of organic xenobiotics in aquatic ecosystems: quantitative and qualitative differences in biotransformation by invertebrates and fish", Comp. Biochem. Physiol, 120A, pp.43~49 (1998)
- Lowry OH, "Protein measurement with the Folin phenol reagent", J. Biol. Chem, 193, pp.265~275 (1951)
- pyrene, "Toxic. in Vitro", 1992, 523~531
- Omura T, "The carbon monoxide-binding pigment of liver microsomes", J. Biol. Chem, 239, pp.2370~2378 (1964)
- Omura T, "A new method for simultaneous purification of cytochrome b5 and NADPH-cytochrome c reductase from rat liver microsomes", J. Biochem, 67, pp.249~257 (1970)
- Pawar KR, "Effect of sumithion on the ovaries of freshwater fish Garra mullya (Sykes)", Curr. Sci, 16, pp.784~785 (1983)
- Philips AH, "Hepatic triphosphopyridine nucleotide-cytochrome c reductase: isolation, characterization, and kinetic studies", J. Biol. Chem, 237, pp.2652~2660 (1962)
- Ram RJ, "Effect of mercuric chloride on the reproductive cycle of the teleostean fish Channa punctatus", Bull. Environ. Contam. Toxicol, 30, pp.24~27 (1983)
- Schlezinger JJ, "Uncoupling of cytochrome P450 1A and stimulation of reactive oxygen species production by co-planar polychlorinated biphenyl congeners", Aquat. Toxicol, 77, pp.422~432 (2006)
- Shukla JP, "Ovarian recrudescence in a freshawter teleost, Saratherodon mossambicus in DDT treatment and pattern of recoupment", J. Environ. Biol, 6, pp.195~204 (1985)
- Thomas P, "Reproductive endocrine function in female atlantic croaker exposed to pollutants", Mar. Environ. Res, 24, pp.179~183 (1988)
- Thomas P, "Teleost model for studying the effects of chemicals on female reproductive endocrine function", J. Exp. Zool, (Suppl.4), pp.126~128 (1990)
- ven der Oost A, "Biomonitoring of aquatic pollution with feral eel (Anguilla angullid) II. Biomarkers: pollution-induced biochemical responses", Aquat. Toxicol, 36, pp.189~222 (1996)
