본문 바로가기
KERD
논문KCI 등재

Saccharomyces cerevisiae에서 N-acetyl-L-cysteine 처리와 감마선 조사에 따른 Glutathione Peroxidase 유전자 발현

Gene Expression of Glutathione Peroxidase in Saccharomyces cerevisiae Treated with N-acetyl-L-cysteine and Gamma-rays

김진규|박지영|백동원|모하마드닐리
한국환경생물학회 (Korean Society Of Environmental Biology)|2011.12|환경생물 (Korean Journal of Environmental Biology)|제29권 제4호|pp. 258-264

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

gene expressionreactive oxygen speciesN-acetyl-L-cysteineglutathione peroxidasegamma-rays

초록

Glutathione (GSH)은 직접적으로 활성산소종을 제거하거나 GSH peroxidase와 같은 활성산소종 제거 효소의 조효소로써, 산화적 스트레스로부터 세포를 방어하는 데 중요한 역할을 한다. GSH peroxidase는 두 분자의 GSH을이용해 세포 내 과산화수소를 물로 전환한다. N-acetyl-L cysteine (NAC)는 항산화제 중 하나로 세포 내 GSH의 전구물질로 이용된다. 본 연구는, 0mM에서 20mM의 NAC 단독 처리 또는 100 Gy 감마선과 복합 처리한 효모세포에서 GSH peroxidase를 코드화(encoding)하는 유전자인GPX1과 GPX2의 전사적 발현을 통해 GSH, NAC와 GSH peroxidase의 연관성을 알아보았다. GPX1과 GPX2의 전사적 발현은 NAC와 100 Gy 감마선에 의해 유도되었다. 조사된 효모세포에서 NAC의 증가 농도에 따라 GSH peroxidase 두 유전자의 발현은 감소되었다. 이러한 결과로, NAC에 의해 증가된 세포 내 GSH는 GSH peroxidase 유전자의 전사적 발현을 유도하며, NAC는 감마선으로부터 생성된 활성산소종 직접적 제거와 GSH peroxidase 유전자의 전사적 발현을 유도함으로써 세포를 보호할 수있다는 것이 밝혀졌다.

Abstract

Glutathione (GSH) has important roles in cellular defense against oxidative stress, 1)direct scavenging of reactive oxygen species (ROS), and 2) coenzyme of ROS scavenging enzyme like glutathione peroxidases (GPx). GSH peroxidase reduces free hydrogen peroxide to water using 2GSH. N-acetyl-L-cysteine (NAC), one of the antioxidants, is used as a precursor for intracellular GSH. In this study, relation of GSH, NAC, and GSH peroxidase was investigated through transcriptional expression of GPX1 and GPX2, which are GSH peroxidase encoding genes, in yeast cells treated with 0 mM to 20 mM of NAC or in combination with 100 Gy gamma-rays. The transcriptional expression of GPX1 and GPX2 was induced by NAC and 100 Gy gamma-rays. The gene expression of both GSH peroxidases was decreased with increasing concentrations of NAC in irradiated yeast cells. These results suggest that elevation of intracellular GSH by NAC and oxidative stress and ROS generated from gamma-rays induces expression of GSH peroxidase genes, and that NAC can protect the yeast cells against ROS generated from gamma-rays through direct scavenging of ROS and transcriptional activation of GSH peroxidase.

참고문헌 (30)

  1. 박지영, "Saccharomyces cerevisiae에서 이온화 방사선과 N-acetyl-L-cysteine 처리에 따른 세포 생존과 Superoxide Dismutase와 Catalase 유전자 발현", 환경생물, 29(1), pp.61~67 (2011), 한국환경생물학회
  2. Aruoma OI, "The antioxidant action of N-acetylcysteineL its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid", Free Radical Biology & Medicine, 6, pp.593~597 (1989)
  3. Atkuri KR, "N-Acetylcysteine- a safe antidote for cystene", glutathione deficiency, 2007, pp.7, 1~5
  4. Berg JM, "Biochemistry, Fifth edition", pp.506~ (2004)
  5. Brennan RJ, "Persistent genomic instability in the yeast Saccharomyces cerevisiae induced by ionizing radiation and DNA-damaging agents", Radiation Research, 155, pp.768~777 (2001)
  6. Circu ML, "Glutathione and apoptosis", Free Radical Research, 42, pp.689~706 (2008)
  7. Davis W, "Cellular thiols and reactive oxygen species in drug induced apoptosis", Perspectives in Pharmacology, 296 (2001)
  8. Delaunay A, "A thiol peroxidase is an H2O2 receptor and redoxtransducer in gene activation", Cell, 111, pp.471~481 (2002)
  9. Dickinson DA, "Cellular glutathione and thiols metabolism", Biochemical Pharmacology, 64, pp.1019~1026 (2002)
  10. Esnault M, "Ionizing radiation: Advances in plant response", Environmental and Experimental Botany, 68, pp.231~237 (2010)
  11. Friesen C, "A critical role of glutathione in determining apoptosis sensitivity and resistance in leukemia cells", Cell Death & Differentiation, 11, pp.S73~S85 (2004)
  12. Inoue Y, "Genetic analysis of glutathione peroxidase in oxidative stress response of Saccharomyces cerevisiae", Journal of Biological Chemistry, 274, pp.27002~27009 (1999)
  13. Jamieson DJ, "Oxidative stress responses of the yeast Saccharomyces cerevisiae", Yeast, 14, pp.1511~1527 (1998)
  14. Kataoka Y, "Relationship between phosphorylated histone H2AX formation and cell survival in human microvascular endothelial cells (HMEC) as a function of ionizing radiation exposure in the presence or absence of thiol-containing drugs", Radiation Research, 168, pp.106~114 (2007)
  15. 강성만, "Glutathione peroxidase 3 of Saccharomyces cereviside regulates the activity of methionine sulfoxide reductase in a redox state-dependent way", BIOCHEM BIOPHYS RES COMMUN, 348, pp.25~35
  16. 김승수, "N-Acetylcysteine Induces Cell Cycle Arrest in Hepatic Stellate Cells through Its Reducing Activity", JOURNAL OF BIOLOGICAL CHEMISTRY, 276(44), pp.40591~40598, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
  17. Musallam L, "Resistance to Fas-induced apoptosis in hepatocytes: role of GSH depletion by cell isolation and culture", AJP - Gastrointestinal and Liver Physiology, 283, pp.G709~G718 (2002)
  18. Nakamura H, "Redox regulation of cellular activation", Annual Review of Immunology, 15, pp.351~369 (1997)
  19. Neal R, "Antioxidant role of N-acetyl cysteine isomers following high dose irradiation", Free Radical Biology & Medicine, 34, pp.689~695 (2003)
  20. Phelps DT, "Elevation of glutathione levels in bovine pulmonary artery endothelial cells by N-acetylcysteine", American Journal of Respiratory Cell and Molecular Biology, 7, pp.293~299 (1992)
  21. Pias EK, "Apoptosis in mitotic competent undifferentiated cells is induced by cellular redox imbalance independent of reactive oxygen species production", Raseb. Journal, 16, pp.781~790 (2002)
  22. Pocernich CB, "In-vivo glutathione elevation protects against hydroxyl free radicalinduced protein oxidation in rat brain", Neurochemistry International, 36, pp.185~191 (2000)
  23. Powis G, "Redox signaling and the control of cell growth and death", Pharmacology & Therapeutics, 68, pp.149~173 (1995)
  24. Raftos JE, "Kinetics of uptake and deacetylation of N-acetylcysteine by human erythrocytes", International Journal of Biochemistry & Cell Biology, 39, pp.1698~1706 (2007)
  25. Reliene R, "N-acetyl cysteine protects against ionizing radiation-induced DNA damage but not against cell killing in yeast and mammals", Mutation Research, 665, pp.37~43 (2009)
  26. Schroeder CP, "Glutathione and drug resistance", Cancer Investigation, 14, pp.158~168 (1996)
  27. Sies H, "Oxidative stress: oxidant and antioxidants", Experimental Physiology, 82, pp.291~295 (1997)
  28. Sjodin K, "Metabolism of N-acetyl-L-cysteine. Some structural requirements for the deacetylation and consequences for the oral bioavailability", Biochemical Pharmacology, 38, pp.3981~3985 (1989)
  29. Ward JF, "Biochemistry of DNA lesions", Radiation Research Supplement, 8, pp.S103~111 (1985)
  30. Watson RW, "Augmented intracellular glutathione inhibits Fastriggered apoptosis of activated human neutrophils", Blood, 89, pp.4175~4181 (1997)

관련 논문AI추천

Saccharomyces cerevisiae에서 이온화 방사선과 N-acetyl-L-cysteine 처리에 따른 세포 생존과 Superoxide Dismutase와 Catalase 유전자 발현김진규, 백동원, Jiyoung Park, Mohammad Nili한국환경생물학회 · 환경생물2011 · 제29권 제1호방사선과 염화수은의 일시 및 반복 복합 처리된 효모세포의 산화적 스트레스 적응과 형태 변화김수현, 김진규한국환경생물학회 · 환경생물2010 · 제28권 제2호Distinct functional roles of peroxiredoxin isozymes and glutathione peroxidase from fission yeast, Schizosaccharomyces pombe김강화, 채호준, Ji Sun Kim, Songmi Lee생화학분자생물학회 · BMB Reports2010 · 제43권 제3호Saccharomyces cerevisiae TSA1의 보존된 아스파트산 잔기 및 세린 잔기의 변이가 과산화효소 활성 및 샤페론 활성에 미치는 영향김강화, 이송미, 조은이한국미생물·생명공학회 · 한국미생물·생명공학회지2017 · 제45권 제1호Lactobacillus spp. 의 항산화 효과 및 활성산소에 대한 내성김현수, 안종남, 이종문, 정석근, 채현석, 함준상한국축산학회 · 한국축산학회지2004 · 제46권 제6호Yeast Prx의 스트레스의존 구조적 변화의 기작강지선, 정강원한국현미경학회 · Applied Microscopy2005 · 제35권 제4호

이 간행물의 다른 논문 (16건)

전자기파의 생체 위해성에 관한 소고정경아, 계명찬pp. 241-250코발트(II)가 말똥성게(Hemicentrotus pulcherrimus)의 수정 및 배아 발생에 미치는 영향류향미, 황운기, 강한승, 최용환, 이승민pp. 251-257Acidophilic Bacterial Communities of Soil and Enrichment Cultures from Two Abandoned Mine Sites of the Korean PeninsulaDong Jin Kim, Sun Hee Lee, 이영하, Jae Hee Kim, Debaraj Mishrapp. 265-273종자크기에 따른 참나무 4종의 발아율, 초기 생장율과 생존율 변화유영한, 신정훈pp. 274-279서식지에 따른 사철나무 잎의 이온화 방사선 조사 후 항산화 활성 변화 차이차민경, 김진규, 나디아빌헬모바, 아니타무크헤르지pp. 280-285
원문 보기