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비스페놀 A에 대한 기수산 물벼룩의 항산화 시스템의 변화

Modulation of antioxidant defense system in the brackish water flea Diaphanosoma celebensis exposed to bisphenol A

김혜리|이영미|유제원|차주선|표진우
한국환경생물학회 (Korean Society Of Environmental Biology)|2019.03|환경생물 (Korean Journal of Environmental Biology)|제37권 제1호|pp. 72-81|DOI 10.11626/KJEB.2019.37.1.072

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bisphenol Aantioxidantswater fleabrackishDiaphanosoma celebensis

초록

Bisphenol A (BPA)는 대표적인 내분비계장애물질로 수서생물의 성장, 발생, 그리고 생식에 유해한 영향을 주는것으로 알려져 있다. 본 연구는 기수산 물벼룩 (Diaphanosoma celebensis)에서 48시간 BPA 노출 후 산화적 스트레스 반응을 조사하기 위하여 qRT-PCR을 이용한 항산화 유전자 발현 변화, 항산화 효소 활성, 총 단백질 함량 분석을 수행하였다. 3 mg L-1의 BPA에 48시간 노출된 D. celebensis에서 모든 항산화 유전자 (Cu/Zn-SOD, Mn-SOD, CAT)의 발현량이 유의하게 증가하였다. 특히 세 종류의 GST isoforms (GST-kappa, GST-mu, GST-theta)는 가장 낮은 농도인 0.12 mg L-1 BPA에 48시간 노출된 실험군에서도 유의하게 증가하였으며, GST-mu의 발현양이 상대적으로 가장 높게 나타났다. SOD 활성은 BPA 농도에 의존적으로 유의하게 증가하였으며, 총 단백질 함량은 BPA 노출에 대해 감소되는 양상을 보였다. 이러한 결과는 BPA가 D. celebensis에서 산화적 스트레스를 유발하였고, 이 과정에서 이들 항산화 유전자가 생물방어기전으로 참여한다는 것을 의미한다. 본 연구는 BPA가 해양생물에 미치는 영향에 대한 분자적 기전을 이해하는 데 도움이 될 것이다.

Abstract

Bisphenol A (BPA), a representative endocrine disrupting chemicals, has adverse effects on growth, development and reproduction in aquatic organisms. The object of this study was to investigate the modulation of antioxidant enzyme – coding genes using quantitative real time RT - PCR (qRT - PCR), enzyme activity and total protein content, to understand oxidative stress responses after exposure to BPA for 48 h in brackish water flea Diaphanosoma celebensis. The BPA (3 mg L-1) significantly upregulated the expression of Cu / Zn - SOD, Mn-SOD, and catalase (CAT ) mRNA. Three GST isoforms (GST-kappa, GST-mu, and GST-theta) mRNA levels significantly increased at the rate of 0.12 mg L-1 of BPA. In particular, GST-mu showed the highest expression level, indicating its key role in antioxidant response to BPA. SOD activity was induced with a concentration - dependent manner, and total protein contents was reduced. These findings indicate that BPA can induce oxidative stress in this species, and these antioxidants may be involved in cellular protection against BPA exposure. This study will provide a better understanding of molecular mode of action of BPA toxicity in aquatic organisms.

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