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대왕범바리 (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂)의 아질산 급성노출에 따른 내성한계: 혈액성상 및 혈장성분의 변화

Tolerance limit of nitrite exposure to hybrid grouper (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂): hematological parameters and plasma components

김준환|이경미|김석렬|조재황|허영백
한국환경생물학회 (Korean Society Of Environmental Biology)|2020.03|환경생물 (Korean Journal of Environmental Biology)|제38권 제1호|pp. 93-100|DOI 10.11626/KJEB.2020.38.1.093

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참고문헌

키워드

nitritehybrid grouperplasma componentshematological parameter

초록

본 실험에서 아질산 급성노출은 대왕범바리의 혈액학적 성상 및 혈장성분에 유의적 변화를 나타내었다. 혈액학적 성상인 Hct와 Hb는 아질산 노출에 의한 유의적 감소를 확인하였다. 혈장 성분인 glucose, cholesterol, GPT 및 ALP는 아질산 노출에 의해 유의적으로 변화를 나타냄을 확인하였다. 본 실험의 결과 아질산 노출 100 mg L-1 이상의 농도는 대왕범바리의 혈액 성상 및 혈장 성분의 유의적 영향을 미치며, 800 mg L-1의 아질산 급성 노출은 대량 폐사를 유발할 수 있으나, 기존 국내 양식 대상종인 Olive flounder, P. Olivaceus는 171.043 mg L-1 (Kim et al. 2018), Yellow tail, Seriola quinqueradiata는 147 mg L-1 (Sugiyama et al. 1991)에 비해 상대적으로 높은 값을 보여 아질산 내성이 상대적으로 강함을 확인할 수 있었다.

Abstract

Hybrid grouper (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂) (mean weight 27.3±3.8 g, mean length 11.6±0.7 cm) were exposed to waterborne nitrite at 0, 100, 200, 400, 800, and 1600 mg L-1 for 96 hours. The hematocrit and hemoglobin values were significantly decreased by exposure to 100 mg L-1 and 400 mg L-1, respectively. In plasma components, no significant change was observed in magnesium. Glucose was significantly increased by 200 and 400 mg L-1 nitrite but reduced by 800 mg L-1. Cholesterol was significantly decreased by 400 mg L-1 nitrite, but there was no significant change in total protein. GPT (glutamic pyruvate transaminase) was significantly increased by exposure to 200 and 400 mg L-1. ALP (Alkaline phosphatase) was significantly increased by 800 mg L-1. The results of this study indicate that acute exposure to nitrite changes physiological parameters, such as hematological properties and plasma components.

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