MSPD와 HPLC를 이용한 돼지고기 및 광어 중의 테트라싸이클린계 항생제와 sulfonamide의 다성분 잔류분석법 개발
Simultaneous Determination of Tetracyclines and Sulfonamides Residues in Pork and Flatfish Using Matrix Solid Phase Dispersion (MSPD) Extraction and HPLC
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초록
This study was conducted to develop more convenient simultaneous determination method by matrix solid phase dispersion (MSPD) and HPLC for sulfonamides such as sulfamerazine (SMR), sulfamethazine (SMT), sulfamonomethoxine (SMM), sulfadimethoxine (SDM), sulfaquinoxaline (SQX), and tetracyclines including oxytetracycline (OTC), tetracycline (TC) chlortetracycline (CTC) in prok and flatfish. The limits of detection were 0.047 ㎎․㎏-1 for OTC, TC, SMR, SMT and SMM, and 0.033 ㎎․㎏-1 for CTC, SDM, and SQX, respectively. So it is sufficiently possible to detect the eight tetracyclines and sulfonamides under their MRLs (0.1~0.2 mg․kg-1). The average percentage recoveries of sulfonamides and tetracyclines from pig muscle and flatfish spiked standard solution were approximately 80.25~101.25% and 85.77~121.42%, respectively. Therefore this method was efficient for simultaneous analysis of eight tetracyclines and sulfonamides.
Abstract
This study was conducted to develop more convenient simultaneous determination method by matrix solid phase dispersion (MSPD) and HPLC for sulfonamides such as sulfamerazine (SMR), sulfamethazine (SMT), sulfamonomethoxine (SMM), sulfadimethoxine (SDM), sulfaquinoxaline (SQX), and tetracyclines including oxytetracycline (OTC), tetracycline (TC) chlortetracycline (CTC) in prok and flatfish. The limits of detection were 0.047 ㎎․㎏-1 for OTC, TC, SMR, SMT and SMM, and 0.033 ㎎․㎏-1 for CTC, SDM, and SQX, respectively. So it is sufficiently possible to detect the eight tetracyclines and sulfonamides under their MRLs (0.1~0.2 mg․kg-1). The average percentage recoveries of sulfonamides and tetracyclines from pig muscle and flatfish spiked standard solution were approximately 80.25~101.25% and 85.77~121.42%, respectively. Therefore this method was efficient for simultaneous analysis of eight tetracyclines and sulfonamides.
참고문헌 (28)
- Heo, G. J., "Diseases of aquaculture animals and prevention of drug residues", Kor. J. Food Hygiene, 7(2), pp.7~9 (1992)
- Hah, D. S., "Simultaneous quantification of sulfonamide and tetracyclines in fish muscle tissue by matrix solid phase dispersion (MSPD) extraction and HPLC", J. Fd. Hyg. safety, 12(2), pp.117~124 (1997)
- KFDA, "Korea food code, Attached table 7", pp.807~809 (2008)
- Kang, H. G., "Multiresidue matrix solid phase dispersion (MSPD) extraction and HPLC determination of tetracyclines in animal muscle tissue", Korean J. Vet. Res., 36(3), pp.541~550 (1996)
- Baker, S. A., "Chromatographic methods for tetracycline analysis in food", J. Chrom., 624, pp.195~209 (1992)
- Bevill, R. F., "Disposition of sulfonamides in food producing animals : Concentrations of sulfamethazine and its metabolites in plasma, urine and tissues of lambs following intravenous administration", Am. J. Vet. Res., 38, pp.973~977 (1977)
- Blood, D. C., "Verterinary medicine 5th ed.", pp.87~90 (1979), Cassel Ltd.
- Tsai, C. E., "Simple continuous and simultaneous determination of tetracycline residue", Research in Vet. Science, 56, pp.277~283 (1994)
- Ryu, J. C., "A study on the determination of residual antibiotics and synthetic antibacterial agents in meat (1)- microbiological assay", Kor. J. Food Hygiene, 8(1), pp.1~8 (1993)
- Yoshimura, H., "Residues of doxycycline and oxytetracycline in eggs after medication via drinking water to laying hen", Food Addit. Contam., 8, pp.65~69 (1991)
- Horwits, W., "Review of analytical method for sulfonamides analytical methods for sulfonamides in food and feeds", J. Assoc. Off. Anal. Chem., 64(1), pp.104~130 (1981)
- Long, A. R., "Matrix solid phase dispersion (MSPD) isolation and liquid chromatographic determination of oxytetracycline, tetracycline, chlorotetracycline in milk", J. Assoc. Off. Ana. Chem., 73(3), pp.379~384 (1990)
- Park, J. M., "Simultaneous determination of tetracyclines in beef, pork and chicken by high performance liquid chromatography using matrix solid phase dispersion extraction", Kor. J. Vet. Publ. Health., 17(3), pp.321~328 (1993)
- Baker, S. A., "Isolation of drug residues from tissues by solid phase dispersion", J. Chrom., 475, pp.353~36 (1989)
- Austin, R. L., "Matrix solid phase dispersion and gas chromatographic screening of nine chlorinated pesticides in beef fat", J. Assoc. Off. Anal. Chem., 74(3), pp.493~496 (1991)
- Smedley M. D., "Liquid chromatographic determination of multiple sulfonamide residues in bovine milk", J. Assoc. Off. Anal. Chem., 73(6), pp.875~879 (1990)
- Onji, Y., "Liquid chromatographic determination of tetracycline residues in meat and fish", J. Assoc. Off. Anal. Chem., 67(6), pp.1135~1137 (1984)
- Oka, H., "Improvement of chemical analysis of antibiotics, part XIX, chromatography and thin layer chromatography", fast atom bombardment mass spectrophotometry, 1994, pp.42, 615~622
- Jung, K. S., "Simultaneous determination of residual synthetic antimicrobials in animal muscles by high performance liquid chromatography", Kor. J. Food Hygiene, 8(1), pp.25~35 (1993)
- AOAC, "Official methods of analysis of AOAC international Vol. 1 Chapter 23", pp.18~19 (2005), Chapter
- Horie, M., "Simultaneous determination of residual synthetic antibacterials in fish by high performance liquid chromatography", J. Chrom., 538, pp.484~491 (1991)
- Long, A. R., "Multiresidue method for the determination of sulfonamides in pork tissue", J. Agric. Food Chem., 38(2), pp.423~426 (1990)
- Lu, H. T, "Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high performance liquid chromatography with fluorescence detection", Chromatographia, 60(5-6), pp.259~264 (2004)
- Reimer, G. J., "Liquid chromatographic confirmatory method for five sulfonamides in salmon muscle tissue by matrix solid-phase dispersion", J. Assoc. Off. Anal. Chem., 75(6), pp.979~981 (1992)
- Baker, S. A., "Preparation of milk sample for immunoassay and liquid chromatographic screening using MSPD", J. Assoc. Off. Anal. Chem., 77, pp.848~854 (1994)
- Booth, N. H., "Drug and chemical residues in edible tissues of animals : Verterinary pharmacology and therapeutics 6th ed", pp.1149~1201 (1988), Iowa State University
- Long, A. R., "Matrix solid phase dispersion isolation and liquid chromatographic determination of oxytetracycline in catfish muscle tissue", J. Assoc. Off. Anal. Chem., 73, pp.864~867 (1990)
- Park, J. J., "A study on the sulfonamide residues in bovin, swine, chicken by high performance liquid chromatography", Kor. J. Vet. Publ. Health., 18(4), pp.317~326 (1994)