국내 수수 종자 분석을 위한 프로테오믹스-기반 바이오마커 개발
Development of Proteomics-based Biomarkers for 4 Korean Cultivars of Sorghum Seeds (Sorghum bicolor (L.) Moench)
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BACKGROUND: Sorghum (Sorghum bicolor (L.) Moench)ranks as the 6th most planted crop in the world behindwheat, rice, maize, soybean, and barley. The objective ofthis study was to identify bio‐marker among sorghumcultivars using proteomics approach such as twodimensionalpolyacrylamide gel electrophoresis (2‐DE)coupled with mass spectrometry (MS). METHODS AND RESULTS: Proteins were extractedfrom sorghum seed, and separated by 2‐DE. Total 652 spotswere detected from 4 different sorghum seed after stainingof 2‐DE with colloidal Coomassie brilliant blue (CBB). Among them, 8 spots were differentially expressed andwere identified using MALDI‐TOF/TOF mass spectrometry. They were involved in RNA metabolism (spot1, spot 4),heat shock proteins (HSPs, spot 2), storage proteins (spot 3,spot 5, and spot 6), and redox related proteins (spot 8). Eightof these proteins were highly up‐regulated in Whinchalsusu(WCS). The HSPs, Cupin family protein, and Globulinwere specifically accumulated in WCS. The DEAD‐box helicase was expressed in 3 cultivars except for WCS. Ribonuclease T2 and aldo‐keto reductase were onlyexpressed in 3 cultivars except for Daepung‐susu (DPS). CONCLUSION(S): Functions of identified proteins weremainly involved in RNA metabolism, heat shock protein(HSP), and redox related protein. Thus, they may providenew insight into a better understanding of thecharactreization between the cultivars of sorghum.
Abstract
BACKGROUND: Sorghum (Sorghum bicolor (L.) Moench)ranks as the 6th most planted crop in the world behindwheat, rice, maize, soybean, and barley. The objective ofthis study was to identify bio‐marker among sorghumcultivars using proteomics approach such as twodimensionalpolyacrylamide gel electrophoresis (2‐DE)coupled with mass spectrometry (MS). METHODS AND RESULTS: Proteins were extractedfrom sorghum seed, and separated by 2‐DE. Total 652 spotswere detected from 4 different sorghum seed after stainingof 2‐DE with colloidal Coomassie brilliant blue (CBB). Among them, 8 spots were differentially expressed andwere identified using MALDI‐TOF/TOF mass spectrometry. They were involved in RNA metabolism (spot1, spot 4),heat shock proteins (HSPs, spot 2), storage proteins (spot 3,spot 5, and spot 6), and redox related proteins (spot 8). Eightof these proteins were highly up‐regulated in Whinchalsusu(WCS). The HSPs, Cupin family protein, and Globulinwere specifically accumulated in WCS. The DEAD‐box helicase was expressed in 3 cultivars except for WCS. Ribonuclease T2 and aldo‐keto reductase were onlyexpressed in 3 cultivars except for Daepung‐susu (DPS). CONCLUSION(S): Functions of identified proteins weremainly involved in RNA metabolism, heat shock protein(HSP), and redox related protein. Thus, they may providenew insight into a better understanding of thecharactreization between the cultivars of sorghum.
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