야관문 에탄올 추출물의 항산화 및 Metalloproteinase 저해 활성
Antioxidant and Metalloproteinase Inhibitory Activities of Ethanol Extracts from Lespedeza cuneata G. Don
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야관문의 항산화 활성과 항주름 활성을 조사하기 위하여30% 에탄올 추출물과 각종 용매 분획물을 이용하여 DPPH 및 ABTS radical 소거활성과 matrix metalloproteinase MMP1과 MMP3 생성억제 효과를 조사하였다. 에탄올 추출물과 각종 용매 분획물 중 에틸아세테이트 분획물에서 페놀및 플라보노이드 함량이 가장 높았다. 야관문 추출물의 항산화 활성과 피부 주름을 유발하는 MMP1, MMP3의 생성을억제 효과는 페놀 및 플라보노이드 함량과 높은 상관관계를보였으며, 페놀과 플라보노이드 함량이 가장 높은 에틸아세테이트 분획물의 항산화 활성과 MMPs 생성 억제 효과가 가장높았다.
Abstract
BACKGROUND: Lespedeza cuneata G. Don is a wellknown medicinal plant. In this study, the biological activities of L. cuneata extracts were investigated. METHODS AND RESULTS: L. cuneata shoot was extracted with 30% ethanol and further fractionated with organic solvents. Total phenolic and flavonoid content, antioxidant activity, andmatrixmetalloproteinase inhibition effect of the extract and fractions were measured. Among the tested extract and fractions, the highest contents of total phenolic and flavonoidswere found in ethyl acetate fraction (117.8 mg GAE/g and 35.9 mg QE/g, respectively). Ethyl acetate fraction showed the highestDPPHandABTS radical scavenging activity, and the antioxidant activity of the other fractions followed the order n-hexane fraction>ethanol extract>methyl chloroform>n-butanol fraction. Inhibitory effect on the expression of matrix metalloproteinases (MMP1 and MMP3) was highest in the fraction of ethyl acetate, and n-butanol fraction also significantly inhibited the expression of MMP3. Antioxidant activities of L. cuneata extracts were significantly positively related to their phenolic and flavonoid content. CONCLUSION: Ethyl acetate fraction of L. cuneata ethanol extract showed potent antioxidant and matrix metalloproteinases inhibitory activities. Those activities might be related to the high total phenolic and flavonoid content of the extract.
참고문헌 (15)
- 차재영, "한방 생약재 발효액의 항산화 활성 및 tyrosinase 저해 활성", 생명과학회지, 20(6), pp.940~947 (2010), 한국생명과학회
- 조영미, "백수오, 우엉, 마 추출물 혼합비율에 따른 혈관부착인자 VCAM-1의 발현억제 효과", 한국자원식물학회지, 29(5), pp.525~531 (2016), 한국자원식물학회[DOI]
- 정희경, "야관문 에탄올 추출물의 자외선 노출에 의한 피부 광노화 개선 효과", 한국식품저장유통학회지, 21(2), pp.264~275 (2014), 한국식품저장유통학회
- 하배진, "한방 원료 추출물의 주름개선 효과를 통한 화장품 원료로서의 가치 평가", 생명과학회지, 17(8), pp.1147~1151 (2007), 한국생명과학회
- 김대익, "흰쥐의 외과적 창상에 대한 야관문 에탄올 추출물의 피부재생 효과", 한국식품영양과학회지, 43(4), pp.516~521 (2014), 한국식품영양과학회
- Kim, J. S., "Isolation and identification of sea buckthorn (Hippophae rhamnoides) phenolics with antioxidant activity and α-glucosidase inhibitory effect", Journal of Agricultural and Food Chemistry, 59(1), pp.138~144 (2011)
- 고현민, "서양금혼초 부위별 추출물의 티로신에이즈, α-글루코시다아제 저해활성 및 항산화효과", 한국약용작물학회지, 25(3), pp.139~145 (2017), 한국약용작물학회[DOI]
- 이혜진, "비수리 추출물의 항균 및 항산화 활성", 한국미생물·생명공학회지, 39(1), pp.63~69 (2011), 한국미생물·생명공학회
- Numata, A., "C-glycosylflavones in Lespedeza cuneata", Chemical and Pharmaceutical Bulletin, 28(3), pp.964~965 (1980)
- Rhie, G. E., "Aging- and photoaging-dependent changes of enzymic and nonenzymic antioxidants in the epidermis and dermis of human skin in vivo", Journal of Investigative Dermatology, 117(5), pp.1212~1217 (2001)
- Senguttuvan, J., "Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities", Asian Pacific Journal of Tropical Biomedicine, 4(Suppl 1), pp.S359~S367 (2014)
- Stich, H. F., "The beneficial and hazardous effects of simple phenolic compounds", Mutation Research, 1991, pp.259(3-4), 307~324
- Tsuji, N., "The role of elastases screted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity", Photochemistry and Photobiology, 74(2), pp.283~290 (2001)
- Wojdylo, A., "Antioxidant activity and phenolic compounds in 32selected herbs", Food Chemistry, 105(3), pp.940~949 (2007)
- Zheng, W., "Antioxidant activity and phenolic compounds in selected herbs", Journal of Agricultural and Food Chemistry, 49(11), pp.5165~5170 (2001)