잠재지문 현출제에 의한 아민계 화합물의 박막 크로마토그래피 상 동정 및 검출한계
Identification and Detection Limit of Amine Family Compounds in Thin-Layer Chromatography by Latent Fingerprint Detectors
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초록
국내에서 불법유포 및 남용으로 문제가 되고 있는 암페타민 류 아민을 함유한 phenethylamine계불법 약물에 대한 쉽고 빠른 동정 및 반 정량적 분석을 목적으로 몇 가지 유사 화합물들을 선정하여 실리카 젤(SiO2)과 알루미나(Al2O3) 박막 크로마토그래피(TLC) 상에서 지문시약을 사용한 발색반응을 확인하였다. 발색반응 후 반응 생성물의 색상을 확인하고 육안으로 검출한계(Limit of Detection, LOD)를 알아보았다. 3가지 지문시약은 TLC상에서 1차, 2차 및 3차 아민에 대하여 각각 특이한 색상을 나타내므로서 암페타민과 메타암페타민의 구별에 유용하게 쓰일 수 있을 것으로 판단된다. 그리고 그 검출한계(LOD)는 각 시료에 대하여 0.1 μg-3 μg (2 μl, per spot)이었으며 지문시약의 종류, 시약농도, 가열시간 및 온도에 의존성을 나타내었다.
Abstract
The various types of amphetamine, which belong to a class of chemicals known as phenethylamines,have been illegally distributed and abused in South Korea. In order to quickly identify, characterize and semiquantitatively determine the presence and type of phenethylamine, several structurally similar compounds were selected and tested in silica gel and alumina thin-layer chromatography (TLC) using fingerprint reagents. Fingerprint reagents produced visible results that could be interpreted with the naked eye and the limit of detection (LOD) was determined in this manner. Three fingerprint reagents generated specific colors on TLC based on the primary, secondary and tertiary amine-type that can be used to differentiate amphetamine and methamphetamine. The LODs of amines were in the range of 0.1-3 μg (2 μl, per spot) based on the type of reagents, concentration of each reagent, heating time and heating temperature.
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