고무로 결합된 과산화수소 감응 탄소반죽 전극의 빠른 감응
Fast Response of the Rubber-bound Carbon Paste Electrode as a Hydrogen Peroxide Sensor
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초록
과산화수소 정량을 위하여 고추냉이의 과산화효소를 효소원으로, 클로로술폰화 고무를 결합재로 사용하여 효소전극을 만들고 전압전류법으로 전기화학적 특성을 살펴보았다. 계가 보여주는 Lineweaver-Burk 도시의 직선성은 H2O2의 분해가 전극표면에 고정된 HRP에 의하여 촉진되고 있었고, 얻어진 대칭인자(α) 및 교환전류밀도(i0)를 포함한 10개 이상의 전기화학 파라미터는 전극이 정량적으로 작동하고 있음을 확인하여 주었다. 특히 2초보다 짧은 전극신호의 포화시간은 지금까지 소개된 과산화수소 감응 탄소반죽전극 중에서 가장 빠른 감응시간이었다. 이 같은 실험적 결과는 법과학 기법처럼 많은 시료의 신속한 분석을 요구하는 화학분석 분야에 응용될 수 있는 이론적인 기초를 제공할 것이다.
Abstract
A chemically modified enzyme electrode specific for hydrogen peroxide, which was bound with chlorosulphonated rubber, was designed by immobilizing horseradish peroxidase as a zymogen and its electrochemical behaviors were evaluated. A straight line of a Lineweaver-Burk plot implied that the dissociation of H2O2 is catalyzed by horseradish peroxidase embedded on the surface of the electrode. And the small symmetry factor (α), the big exchange current density (i0) and the other electrochemical parameters more than10 affirmed that the electrode operates quantitatively. Especially the saturation time of the electrical signal shorter than 2 s is the fastest one among the response times published till quite recently. The experimental results collected here will furnish a theoretical basis to the fields of the forensic methodology and others that require a speedy and plenty analysis.
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