Garlic EST DB를 이용한 국내산과 중국산 마늘의 구분 마커 개발방법
Classification Method about Domestic and Chinese Garlic (Allium sativum L.) Using Garlic EST DB
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
최근, 농산물 개방에 따라 국산과 외형상 유사하나 가격이 저렴한 중국산 마늘이 대량 수입되고 있다. 공식적인 수입의 경우에는 국내 경제에 영향을 미치며 비공식적인 수입은 안전성 문제와 국내산으로 둔갑하여 원산지 표시제 위반 등의 문제를 야기한다. 이에 따라 국내산과 중국산 마늘을 구분하기 위한 방법을 제안하고자 생물정보학적 분석법(Garlic EST DB)을 이용하여 국내산과 중국산 마늘에서 차이를 보이는 영역 107개를 동정하였다. 이중 PCR법으로 적용 가능한 47개의 프라이머를 제작하고 실험에 적용하여 확인한 결과, Garlic 10, Garlic 14, Garlic 18, c10.3 및 g6 총 5개의 마커 후보군을 선발하였으며 그 활용 여부를 검증하기 위하여 시중에 유통되는 중국산 마늘 5가지를 무작위로 수집하여 실험에 적용하였다. 이를 통하여 선별한 마커 후보군이 국내산 마늘과 중국산 마늘을 구분하는 마커로서 활용할 수 있는 가능성과 한계를 확인하였다. 그러므로 국내산 마늘과 중국산 마늘을 구분 할 수 있는 분자 마커를 더 개발하여 본 연구에서 동정한 5개의 분자 마커 후보군과 함께 적용한다면 좀 더 명확한 구분이 가능 할 것이다.
Abstract
The recent opening of the agricultural market is causing increases in agricultural imports. Therefore, a lot of Chinese garlic that look exactly like domestic garlic are being imported and distributed. This has caused socioeconomic problems in which Chinese garlic appeared to be locally produced. This study was designed to solve this issue by using the identification of the molecular marker using PCR. Using bioinformatics analysis, 107 regions have been identified that distinguished domestic and Chinese garlic. Hence, the author designed 47 primers and applied these primers to verify these regions through experiments. Consequently, these primers distinguished between domestic garlic from Chinese garlic. Finally the candidates of 5 markers were secured. For validation, this study randomly chooses 5 types of Chinese garlic from 5 primer candidates that were applied. As a result, these candidates appeared to have the potential to be markers even if some samples showed patterns of domestic garlic. But, the pattern shown between Chinese garlic and domestic garlic should be applied in other tests to classify between domestic and Chinese garlic. Therefore, further studies are necessary to develop a marker which can distinguish between the Chinese garlic that show a similar pattern with domestic garlic.
참고문헌 (37)
- X Sun, "De novo assembly and characterization of the garlic (Allium sativum) bud transcriptome by Illumina sequencing", Plant Cell Rep, 31, pp.1823~1828 (2012)
- 허만규, "Population structure and genetic diversity of garlic in Korea by ISSR marker", 생명과학회지, 16(2), pp.253~258 (2006), 한국생명과학회
- V Brandolini, "Chemical and genomic combined approach applied to the aharacterization and identification of Italian Allium sativum L", J Agric Food Chem, 53, pp.678~683 (2005)
- 배성국, "한국과 몽고 일부 재배마늘의 유전적 변이와 재배종 특이적 RAPD 마커의 탐색", 한국자원식물학회지, 23(5), pp.458~464 (2010), 한국자원식물학회
- CP Cunha, "New Microsatellite markers for garlic, Allium sativum (Alliaceae)", Am J Botany, pp.e17~e19 (2012)
- E-M Eom, "Cloning and characterization of highly repetitive sequences in the genome of Allium sativum L", J Plant Biol, 39, pp.19~55 (1996)
- H Liu, "Development of DNA markers for hybrid identification in Leucadendron (proteaceae)", Scientia Horticulturae, 113, pp.376~382 (2007)
- 이석하, "DNA 마커의 개발 현황과 이용", 한국채소육종학회, 45(별책1), pp.16~20 (2000)
- M Ipeak, "Comparison of AFLPs, RAPD markers, and isozymes for diversity assessment of garlic and detection of rutative duplicates in germplasm collections", J Amer Soc Hort Sci, 128, pp.246~252 (2003)
- RGF Morales, "Genetic divergence among Brazilian garlic cultivars based on mophological characters and AFLP markers", Genet Mol Res, 12, pp.270~281 (2012)
- K-H Ma, "Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic (Allium sativum L.)", Scientia Horticulturae, 122, pp.355~361 (2009)
- HI MaaB, "Infraspecific differentiation of garlic (Allium sativum L.) by isozyme and RAPD markers", Theor Appl Genet, 91, pp.89~97 (1995)
- N Stein, "A 1,000-loci transcript map of the barley genome: new anchoring points for integrative grass genomics", Theor Appl Genet, 114, pp.823~839 (2007)
- and synteny based on EST-derived SNPs, "PNAS", 2009, 118159~118164
- MD Moccia, "A White Campion (Silene Iatifolia)floral expressed sequence tag (EST) library: annotation, EST-SSR characterization, transferability, and utility for comparative mapping", BMC Genomics, 10, pp.243~ (2009)
- H Luo, "Analysis of expressed sequence tags from the Huperzia serrata leaf for gene discovery in the areas of secondary metabolite biosynthesis and development regulation", Physiologia Plantarum, 139, pp.1~12 (2010)
- Z Wang, "Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas)", BMC Plant Biology, 11, pp.139~ (2011)
- S-L Teh, "Development of expressed sequence tag resources for Vanda Mimi Palmer and data mining for EST-SSR", Mol Biol Rep, 38, pp.3903~3909 (2011)
- GDL Cervigni, "Expressed sequence tag analysis and development of gene associated markers in a near-isogenic plant system of Eragrostis curvula", Plant Mol. Biol, 67, pp.1~10 (2008)
- JR Russell, "Identification, utilisation and mapping of novel transcriptome- based markers from blackcurrant (Ribes nigrum)", BMC Plant Biology, 11, pp.147~ (2011)
- D Sehgal, "Integration of gene-based markers in a pearl millet genetic map for identification of candidate genes underlying drought tolerance quantitative trait loci", BMC Plant Biology, 12, pp.9~ (2012)
- S Ueno, "Microsatellite markers derived from Quercus mongolica var. crispula (Fagaceae) inner bark expressed sequence tags", Genes Genet Syst, 83, pp.179~187 (2008)
- JJ Zhang, "Two EST-derived marker systems for cultivar identification in tree peony", Plant Cell Rep, 31, pp.299~310 (2012)
- PW Chee, "EST derived PCR-based markers for functional gene homologues in cotton", Genome, 47, pp.449~462 (2004)
- D-W Kim, "Garlic ESTdb: an online database and mining tool for garlic EST sequences", BMC Plant Biology, 9, pp.61~ (2009)
- TJ White, "PCR Protocols: A Guide to Methods and Application", pp.315~322 (1990), San Diego Academic Press
- 홍선미, "Garlic EST DB를 이용한 국내종 마늘과 중국종 마늘의 구분 마커 개발", 석사, 과학수사학과 과학수사전공, pp.2014