약쑥의 항염 유효 성분 규명과 탈취 공정에 따른 항염 유효 성분 및 휘발성 향기 성분의 변화
Identification of Anti-inflammatory Active Compounds from Artemisia princeps and Variation on Anti-inflammatory Active and Volatile Flavor Compounds Contents according Deodorization Process
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초록
본 연구에서는 화장품 원료인 약쑥의 항염 유효 성분을 규명하였다. 그리고 다양한 탈취 공정에 따른 항염 유효 성분 및 휘발성 향기 성분의 변화를 연구하였다. 3,4-Di-O-caffeoylquinic acid, jaceosidin 및 eupatilin이 뛰어난 NO 생성량과 전염증성 매개체인 iNOS, COX-2, PGE2, IL-1β, IL-6 및 TNF-α의 생성량이 농도 의존적으로 억제되었다. 탈취 공정 추출물로는 SFE 로 정유 성분을 제거한 65% 에탄올 재추출물과 활성탄, 벤토나이트, 산성백토, GSP-07, 25 및 50를 각각 처리한 65% 에탄올 추출물이었다. 탈취 공정 중, SFE 통하여 정유 성분을 제거한 65% 에탄올 재추출물은 SFE에 의해 항염 유효 성분을 제외한 정유 성분과 화합물들이 제거되어 3,4-di-O-caffeoylquinic acid, jaceosidin 및 eupatilin의 함량이 증가하였다. 이 후, 건조 약쑥, 65% 에탄올 약쑥 추출물과 탈취 공정을 처리한 65% 에탄올 추출물의 휘발성 향기 성분의 변화도 분석하였다. Caryophyllene oxide과 α-terpineol을 제외한 보고된 주요 휘발성 향기 성분은 탈취 공정을 처리한 65% 에탄올 추출물에서 제거되었다. 그 결과, SFE에 의해 정유 성분을 제거한 65% 에탄올 재추출물은 정유 성분 사용의 경제성, 높은 항염 유효 성분 함량 및 휘발성 향기 성분들이 저감되는 가장 효율적인 탈취 공정으로 사료된다.
Abstract
In this study, anti-inflammatory active compounds of Artemisia princeps, as a cosmetic raw material, were identified. Furthermore, anti-inflammatory active compounds and volatile flavor compounds contents variation by various deodorization were investigated. The 3,4-di-O-caffeoylquinic acid, jaceosidin, and eupatilin were reduced significantly NO production and proinflammatory mediators, such as iNOS, COX-2, PGE2, IL-1β, IL-6, and TNF-α, production in a concentration dependent manner. Deodorization process extracts were 65% EtOH re-extract that removed essential oil by SFE and 65% EtOH extracts respectively treated active carbons, bentonites, clay acids, GSP-07, 25, and 50. Among these deodorization process, the 65% EtOH re-extract that removed essential oil by SFE was increased 3,4-di-O-caffeoylquinic acid, jaceosidin, and eupatilin contents because essential oils and compounds were removed excluding anti-inflammatory active compounds. Dried Artemisia princeps, 65% EtOH Artemisia princeps extract and 65% EtOH Artemisia princeps extract treated deodorization were analyzed variation on volatile flavor compounds. The major volatile flavor compounds reported were removed in 65% EtOH Artemisia princeps extract treated deodorization, excluding caryophyllene oxide and α-terpineol. As a result, 65% EtOH re-extract that removed essential oil by SFE suggests that it is the most efficient deodorization process which had economic efficiency of using essential oils, high anti-inflammatory active compounds contents and reduction of volatile flavor compounds.
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