A Study on the Whitening and Anti-wrinkle Effects of Chinese Achyranthes bidentata Blume Root Extract According to Different Extraction Methods
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This study investigated cytotoxicity and the inhibitory effects against the nitric oxide production, tyrosinase, melanogenesis in B16F10 cells, collagenase, and elastase in order to study the anti-inflammatory, whitening and anti-wrinkle activities using various fractions of Achyranthesbidentata.The cell viabilities of various fractions excluding hexane fraction have high safety in raw 264.7 cells. The fractions of Achyranthes bidentata root inhibited tyrosinase, melanogenesis, collagenase, and elastase in a concentration-dependent manner. The inhibitory effect against nitric oxide production for different fractions showed as order of ethyl acetate fraction> butanol fraction> ethanol extract> water fraction> and hexane fraction at 120 ㎍/ml. The maximum inhibitory activities against tyrosinase and melanogenesis were obtained 69.6% at 90 μg/ml and 24.1% at 120 μg/ml of ethyl acetate fraction, respectively. Tyrosinase inhibitory activity which was related to anti-melanogensis was observed. In the case of the collagenase and elastase inhibitory effect, they were obtained 68.2% and 80.2% in 90 μg/ml of butanol fraction, respectively. Throughout all results, these results indicate that the fractions of ethyl acetate and butanol of A. bidentata root, which inhibits melanin synthesis and the expression activities against tyrosinase, collagenase and elastase were an effective skin whitening and anti-wrinkle agents.
Abstract
This study investigated cytotoxicity and the inhibitory effects against the nitric oxide production, tyrosinase, melanogenesis in B16F10 cells, collagenase, and elastase in order to study the anti-inflammatory, whitening and anti-wrinkle activities using various fractions of Achyranthesbidentata.The cell viabilities of various fractions excluding hexane fraction have high safety in raw 264.7 cells. The fractions of Achyranthes bidentata root inhibited tyrosinase, melanogenesis, collagenase, and elastase in a concentration-dependent manner. The inhibitory effect against nitric oxide production for different fractions showed as order of ethyl acetate fraction> butanol fraction> ethanol extract> water fraction> and hexane fraction at 120 ㎍/ml. The maximum inhibitory activities against tyrosinase and melanogenesis were obtained 69.6% at 90 μg/ml and 24.1% at 120 μg/ml of ethyl acetate fraction, respectively. Tyrosinase inhibitory activity which was related to anti-melanogensis was observed. In the case of the collagenase and elastase inhibitory effect, they were obtained 68.2% and 80.2% in 90 μg/ml of butanol fraction, respectively. Throughout all results, these results indicate that the fractions of ethyl acetate and butanol of A. bidentata root, which inhibits melanin synthesis and the expression activities against tyrosinase, collagenase and elastase were an effective skin whitening and anti-wrinkle agents.
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