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TiO2/GOD 혼합물 기반의 글루코스 바이오 센서의 제작과 표면 처리를 통한 감도개선

Fabrication and Improved Sensitivity with Surface Treatment of TiO2/GOD Mixture based Glucose Biosensor

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초록

In this paper, the TiO2/glucose oxidase (GOD) mixture has synthesized through simple and low-cost fabrication methods. The phys- ical properties of the mixture were proved using an FT-IR/NIR spectrometer, an X-Ray diffractometer, and a Raman spectrometer. GOD maintained its bioactivity during all fabrication process. The current characteristics of the glucose biosensor were proportional to the glucose concentration and effective surface area of square pyramid on a silicon substrate. The maximum current change was measured in a pH 7.0 buffer solution. The simple and low-cost fabrication process and surface treatment can be used widely in previous research for improvements in effective surface area.

Abstract

In this paper, the TiO2/glucose oxidase (GOD) mixture has synthesized through simple and low-cost fabrication methods. The phys- ical properties of the mixture were proved using an FT-IR/NIR spectrometer, an X-Ray diffractometer, and a Raman spectrometer. GOD maintained its bioactivity during all fabrication process. The current characteristics of the glucose biosensor were proportional to the glucose concentration and effective surface area of square pyramid on a silicon substrate. The maximum current change was measured in a pH 7.0 buffer solution. The simple and low-cost fabrication process and surface treatment can be used widely in previous research for improvements in effective surface area.

참고문헌 (6)

  1. J. Wang, "Electrochemical glucose biosensors", Chem. Rev., 108(2), pp.814~825 (2007)
  2. A. Heller, "Electrical connection of enzyme redox centers to electrodes", J. Phys. Chem., 96(9), pp.3578~3587 (1992)
  3. L Gorton, "Carbon paste electrodes modified with enzymes, tissues, and cells", Electroanalysis, 7(1), pp.23~45 (1995)[DOI]
  4. P. N. Bartlett, "Kinetic aspects of the use of modified electrodes and mediators in bioelectrochemistry", pp.1~101 (1991), Pergamon Press
  5. M. Viticoli, "Third-generation biosensors based on TiO2 nanostructured films", Mater. Sci. Eng. C, 26(5-7), pp.947~951 (2006)[DOI]
  6. E. Topoglidis, "Protein Adsorption on Nanocrystalline <TEX>$TiO_2$<", TEX> Films: An Immobilization Strategy for Bioanalytical Devices, 1998, pp.70(23), 5111~5113

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