갈근추출액이 알레르기 염증반응에 미치는 영향
The Effects of Puerariae Radix on Allergic Inflammation
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Objectives To study the effects of Puerariae Radix (PR) on allergic inflammation. Methods In vivo, Animals were orally administrated with PR once a day for 5 days. Then, we investigated compound 48/80-induced active systemic anaphylatic shock using ICR mice and anti-DNP IgE-induced passive cutaneous anaphylaxis using Sprague Dawley rat. In vitro, we measured cell viability, b-hexosaminidase release, IL-4, TNF-a and PGE2 secretion and IL-3, IL-4, TNF-a, COX-2 and iNOS mRNA expression from RBL-2H3 cells after treatment of PR. Results In vivo, PR pretreatments inhibited compound 48/80-induced active systemic anaphylatic shock. Passive cutaneous anaphylaxis was inhibited. In vitro, 0 ~ 1% PR treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-a and PGE2 secretion. PR treatments also decreased IL-3, IL-4, TNF-a, COX-2 and iNOS mRNA expression in RBL-2H3 cells. Conclusions These results suggest that PR may be beneficial in the inhibition of allergic inflammatory response.
Abstract
Objectives To study the effects of Puerariae Radix (PR) on allergic inflammation. Methods In vivo, Animals were orally administrated with PR once a day for 5 days. Then, we investigated compound 48/80-induced active systemic anaphylatic shock using ICR mice and anti-DNP IgE-induced passive cutaneous anaphylaxis using Sprague Dawley rat. In vitro, we measured cell viability, b-hexosaminidase release, IL-4, TNF-a and PGE2 secretion and IL-3, IL-4, TNF-a, COX-2 and iNOS mRNA expression from RBL-2H3 cells after treatment of PR. Results In vivo, PR pretreatments inhibited compound 48/80-induced active systemic anaphylatic shock. Passive cutaneous anaphylaxis was inhibited. In vitro, 0 ~ 1% PR treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-a and PGE2 secretion. PR treatments also decreased IL-3, IL-4, TNF-a, COX-2 and iNOS mRNA expression in RBL-2H3 cells. Conclusions These results suggest that PR may be beneficial in the inhibition of allergic inflammatory response.
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