본문 바로가기
KERD
논문KCI 등재

조협 (皂莢, Gleditsiae Fructus) n-hexane 추출물이 NC/Nga Tnd mouse의 아토피 피부염에 미치는 영향

The Effects of Gleditsiae Fructus n-hexane Extract on Atopic Dermatitis of NC/Nga Tnd Mouse

김윤희|구은진
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2021.02|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제35권 제1호|pp. 76-103

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

Atopic dermatitis皂莢 (Gleditsiae Fructus)Echinocystic acidOleanolic acidN-hexaneRaw264.7 cellsNC/Nga Tnd mouse
주제분류한의학

초록

Objectives: This study was designed to examine the effect of Gleditsiae Fructus n-hexane (GSF_Hx) on two different groups (on the LPS-induced activation of Raw264.7 cells in vitro, and on the DNCB-induced activation of atopic dermatitis NC/Nga Tnd mice in vivo) to find index components and active components of Gleditsiae Fructus. Methods: GSF_Hx was analyzed by HPLC profiling and confirmed echinocystic acid (EA), oleanolic acid (OA) as index components of Gleditsiae Fructus. Using GSF_Hx, EA, OA, we investigated IL-6, TNF-α, NO production by ELISA analysis and evaluated manifestations of MAPKs transcription factors and NF-κB p65 translocation by western blotting. During In vivo study, atopic dermatitis was induced on NC/Nga Tnd mice by DNCB and administered GSF_Hx, EA, OA orally, and checked skin lesions and measured skin clinical score. Serum IgE level, Th1 and Th2 cytokines secretion and modulating molecular mediators and immune cells in the spleenocyte culture supernatant, PBMCs, ALN and dorsal skin were also measured by real-time PCR. Then, skin rash was evaluated and mast cell distribution was verified by H&E and toluidine blue staining on dorsal skin. Results: It is possible that GSF_Hx, EA and OA reduce inflammation and allergic response of atopic dermatitis by suppressing Th1 and Th2 cytokines secretion and modulating molecular mediators and immune cells. They also had moisturizing effect by raising vitality of ceramide in dorsal skin of atopic dermatitis NC/Nga Tnd mice. However, EA particularly had better overall activity data than OA, that EA could be a more effective active component of Gleditsiae Fructus than OA. Conclusions: Based on the inflammatory reduction property with moisturizing effect, GSF_Hx may play a role in effective treatment for atopic dermatitis.

Abstract

Objectives: This study was designed to examine the effect of Gleditsiae Fructus n-hexane (GSF_Hx) on two different groups (on the LPS-induced activation of Raw264.7 cells in vitro, and on the DNCB-induced activation of atopic dermatitis NC/Nga Tnd mice in vivo) to find index components and active components of Gleditsiae Fructus. Methods: GSF_Hx was analyzed by HPLC profiling and confirmed echinocystic acid (EA), oleanolic acid (OA) as index components of Gleditsiae Fructus. Using GSF_Hx, EA, OA, we investigated IL-6, TNF-α, NO production by ELISA analysis and evaluated manifestations of MAPKs transcription factors and NF-κB p65 translocation by western blotting. During In vivo study, atopic dermatitis was induced on NC/Nga Tnd mice by DNCB and administered GSF_Hx, EA, OA orally, and checked skin lesions and measured skin clinical score. Serum IgE level, Th1 and Th2 cytokines secretion and modulating molecular mediators and immune cells in the spleenocyte culture supernatant, PBMCs, ALN and dorsal skin were also measured by real-time PCR. Then, skin rash was evaluated and mast cell distribution was verified by H&E and toluidine blue staining on dorsal skin. Results: It is possible that GSF_Hx, EA and OA reduce inflammation and allergic response of atopic dermatitis by suppressing Th1 and Th2 cytokines secretion and modulating molecular mediators and immune cells. They also had moisturizing effect by raising vitality of ceramide in dorsal skin of atopic dermatitis NC/Nga Tnd mice. However, EA particularly had better overall activity data than OA, that EA could be a more effective active component of Gleditsiae Fructus than OA. Conclusions: Based on the inflammatory reduction property with moisturizing effect, GSF_Hx may play a role in effective treatment for atopic dermatitis.

참고문헌 (48)

  1. Hanuigwadaehak Bonchohak Pyeonchanwiwonhoe, "Bonchohak", pp.490~491 (2008), Yeongrimsa Publishing Co
  2. Heo J, "Shindaeyukdonguibokam", pp.597~599 (2009), Bubinmunhwasa Publishing Co
  3. Dai Y, "Antiallergic and Anti-inflammatory properties of the Ethanolic Extract from Gleditsia sinensis", Biol. Pharm. Bull, 25(9), pp.1179~1182 (2002)
  4. Fu LJ, "Inhibition of experimental allergic rhinitis by the n-Butanol fraction from the Anomalous Fruits of Gleditsia sinensis", Biol. Pharm. Bull, 26(7), pp.974~977 (2003)
  5. Lee MY, "Antiasthmatic effects of Gleditsia sinensis in an ovalbumin-induced murine model of asthma", International Journal of Toxicology, 30(5), pp.528~537 (2011)
  6. Choi JY, "Protective effect of the fruit hull of Gleditsia sinensis on LPS-Induced acute lung injury is associated with Nrf2 activation", EvidenceBased Complementary and Alternative Medicine (2012)[DOI]
  7. Kim KH, "The fruit hull of Gleditsia sinensis enhances the anti-tumor effect of cis-diammine dichloridoplatinum II (Cisplatin)", Evidence-Based Complementary and Alternative Medicine (2016)[DOI]
  8. Park DY, "Antioxidant activities of extracts from Gleditsiae fructus", pp.2012
  9. Kim YJ, "Inhibition effect of Gleditsia Sinensis Lam on T tyrosinase activity and reactive oxygen species related to melanin biosynthesis", J. Kor Society for Biotechnol & Bioeng, 4, pp.541~543 (2005)
  10. Lee EO, "Study on 'Chu-Suk'(VI) -Prosapogenin in pods of Gleditschia officinalis", Kor. J. Pharmacog, 9(2), pp.93~97 (1978)
  11. Jung GJ, "Antioxidant effect and Tyrosinase inhibitory activity of the 20 Leguminosae family plants", pp.2012
  12. Kim YZ, "The whitening effect of the mixture of Johyeob and Cheongung", Kor. J. Soc. B&A, 8(2), pp.3~14 (2007)
  13. 박혜정, "항산화 효능을 가진 사포닌이 사람섬유아세포에서 기질 금속 단백질 분해효소에 미치는 영향", 생명과학회지, 21(9), pp.1266~1273 (2011), 한국생명과학회
  14. Joh EH, "Anti-inflammatory effects of saponins derived from natural products", pp.2015
  15. Miyase T, "Bisdesmosidic saponins from fruits of Gleditsia caspica Desf", Rev. Latinoamer. Quim, 37(3), pp.218~229 (2009)
  16. Chen JP, "Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD", Acta Pharm, 63, pp.505~515 (2013)
  17. Ma Z, "Echinocystic Acid Inhibits IL-1β-Induced COX-2 and iNOS Expression in Human Osteoarthritis Chondrocytes", Inflammation, 39(2), pp.543~549 (2016)
  18. Yang JH, "Echinocystic acid inhibits RANKL-induced osteoclastogenesis by regulating NF-κB and ERK signaling pathways", Biochem Biophys Res Commun, 477(4), pp.673~677 (2016)
  19. Lai P, "Echinocystic acid, isolated from Gleditsia sinensis fruit, protects endothelial progenitor cells from damage caused by oxLDL via the Akt", eNOS pathway, 2014, pp.114(2), 62~69
  20. Choi JK, "Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model", Toxicology and Applied Pharmacology, 269, pp.72~80 (2013)
  21. 구은진, "조협 추출물이 HaCaT cells의 항염증과 SEB-1 cells의 피지분비 억제에 미치는 영향", 대한한방소아과학회지, 30(2), pp.96~106 (2016), 대한한방소아과학회
  22. Leung DYM, "New insights into atopic dermatitis", J Clin invest, 113(5), pp.651~657 (2004)
  23. Schneider L, "Atopic dermatitis : A practice parameter update 2012", J Allergy Clin Immunol, 131, pp.295~299 (2013)
  24. 이동훈, "아토피피부염 치료 실태에 관한 다기관 설문조사", Allergy Asthma & Respiratory Disease, 4(4), pp.271~275 (2016), 대한 소아알레르기 호흡기학회[DOI]
  25. Kim MY, "Factors of affecting atopic dermatitis in infant", pp.2015
  26. 나민선, "영아 아토피피부염에서 알레르기 감작과 IgE 매개 여부에따른 임상 양상", Allergy Asthma & Respiratory Disease, 5(3), pp.128~134 (2017), 대한 소아알레르기 호흡기학회[DOI]
  27. Yamamoto M, "A novel Atopic dermatitis model induced by topical aplication with dermatophagoides Farinae extract in NC", Nga Tnd mice, 2007, pp.56(2), 139~148
  28. Park NJ, "The improvement effect of Lobelia chinensis on atopic dermatitis and skin barrier function", pp.2017
  29. Kim DW, "Review of atopic dermatitis", Update in Dermatology, 1(3), pp.14~20 (2003)
  30. Ahn JH, "Efficacy and safety of topical Jaungo application in patients with atopic dermatitis: a randomized, double-blind, placebo-controlled study", pp.2017
  31. 강현구, "아토피피부염 환자에서 국소 스테로이드 사용에 따른 혈청스테로이드 농도 측정", Allergy Asthma & Respiratory Disease, 5(2), pp.79~82 (2017), 대한 소아알레르기 호흡기학회[DOI]
  32. 김혜성, "아토피피부염의 치료", 대한의사협회지, 57(3), pp.226~233 (2014), 대한의사협회[DOI]
  33. Guglielmetti, S, "Atopic keratoconjunctivitis and atopic dermatitis", Current opinion in allergy and clinical immunology, 10(5), pp.478~485 (2010)
  34. 이진용, "Topical Corticosteroid Phobia among Parents of Children with Atopic Eczema in Korea", Annals of Dermatology, 27(5), pp.499~506 (2015), 대한피부과학회
  35. Kim GW, "Effects of ATO-tang on Anti-atopic Dermatitis and Skin Regeneration", pp.2017
  36. Jang KA, "Hankulkyoungakjeonsu (2)", pp.58~ (2011), Hanmiuihak Publishing Co
  37. 김범준, "Macrophage Raw 264.7 세포와 아토피성 피부염이 유도된 NC", Nga Mice에서 AF-343에 의한 염증 억제 효과와 피부 보습 효과에 관한 연구, 2010, pp.15(2), 61~76[DOI]
  38. 노효선, "비만세포에서 백질려 추출물의 항염증효과에 대한 연구", 대한본초학회지, 32(2), pp.107~114 (2017), 대한본초학회[DOI]
  39. Baeuerle PA, "NF-kappa B ten years after", Cell, 87, pp.13~20 (1996)
  40. Aioi A, "Impairment of skin barrier function in NC", Nga Tnd mice as a possible model for atopic dermatitis, 2001, pp.144(1), 12~18
  41. Vestergaard C, "The NC", Nga mouse: a model for atopic dermatitis, 2000, pp.5(1), 209~210
  42. 이진화, "連翹 n-BuOH 분획물의 아토피 피부염 억제 효과", 대한한방소아과학회지, 30(3), pp.1~30 (2016), 대한한방소아과학회
  43. 강기연, "杏仁 분획물이 Th2 cytokine 발현과 NC", Nga mouse의 아토피 피부염에 미치는 영향, 2016, pp.30(4), 29~59[DOI]
  44. 김규종, "만성 위축성 위염에서 TGF-β1의 발현", Clinical Endoscopy, 33(5), pp.271~277 (2006), 대한소화기내시경학회
  45. Park JH, "Opposing roles of TGF-β in IL-1β-induced cyclooxygenase-2 expression in follicular dendritic cell-like cells", pp.2016
  46. 김성호, "連翹升麻湯이 함유된 한방화장품이 아토피 피부염에 미치는 임상 연구", 한방안이비인후피부과학회지, 21(2), pp.126~141 (2008), 대한한방안이비인후피부과학회
  47. Kitatani K, "Involvement of acid β-Glucosidase 1 in the salvage pathway of ceramide formation", J Biol Chem, 284(19), pp.12972~12978 (2009)
  48. Sonkoly E, "IL-31 : A new link between T cells and pruritus in atopic skin inflammation", J Allergy clin immunol, 177(2), pp.411~417 (2006)

관련 논문AI추천

加味消風散이 염증 및 아토피피부염 동물병태에 미치는 영향김동희, 박지원, 심부용, 이해진한의병리학회 · 동의생리병리학회지2014 · 제28권 제2호連翹 n-BuOH 분획물의 아토피 피부염 억제 효과김윤희, 이진화, 한재경대한한방소아과학회 · 대한한방소아과학회지2016 · 제30권 제3호白鮮皮 추출물이 DNCB로 유발된 NC/Nga 생쥐의 아토피 피부염에 미치는 영향김윤희, 이윤실, 한재경대한한방소아과학회 · 대한한방소아과학회지2014 · 제28권 제4호DNCB로 유발된 아토피 피부염 동물 모델에 대한 藿香正氣散 경구투여의 효과김영은, 박성환, 손미주, 이소민, 정지연대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2019 · 제32권 제2호황련-감초 추출물을 이용한 하태독법이 NC/Nga 생쥐에서 유발된 아토피 유사 피부염에 미치는 항염증 효과김기봉, 박선영, 안상현, 정아람, 차호열, 천진홍, 최준용대한한방내과학회 · 대한한방내과학회지2015 · 제36권 제4호杏仁 분획물이 Th2 cytokine 발현과 NC/Nga mouse의 아토피 피부염에 미치는 영향강기연, 김윤희, 한재경대한한방소아과학회 · 대한한방소아과학회지2016 · 제30권 제4호

이 간행물의 다른 논문 (9건)

특발성 저신장의 변증 유형 및 변증별 증상 분석 -중의학 논문을 중심으로-권찬영, 장수빈, 이보람pp. 1-17소아 성장에 대한 한의학 치료: 국내 임상연구에 대한 문헌적 고찰박장경, 안뜰에봄, 연지혜, 성현경, 성수현, 한지은pp. 18-29궤양성 대장염 유발 생쥐에서 두시추출물의 항염증 효과양병목, 안상현, 김기봉pp. 30-39황금추출물의 지방장벽 생성을 통한 염증완화 효과김기봉, 안상현pp. 40-47중의학 데이터베이스 (CAJ)를 이용한 급성백혈병의 한약치료에 대한 임상 연구 동향김정은, 김기봉, 천진홍, 장진우, 박범찬pp. 48-62
원문 보기