본문 바로가기
KERD
논문KCI 등재

항염증조절을 통한 금은화-감초 복합 추출물의 DSS 유도 궤양성 대장염 완화 효과

The Anti-Inflammatory Effect of Lonicera Japonica-Glycyrrhiza Uralensis Decoction on Ulcerative Colitis Induced by DSS in Mice

김기봉|안상현|김호현|이연우
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2018.08|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제32권 제3호|pp. 16-25|DOI https://doi.org/10.7778/jpkm.2018.32.3.0016

1

조회

0

다운로드

6

인용

0

참고문헌

키워드

Ulcerative colitisGlycyrrhiza uralensisAnti-inflammationLonicera japonica
주제분류한의학

초록

Objectives The purpose of this study is to investigate the anti-inflammatory effect of Lonicera Japonica-Glycyrrhiza Uralensis decoction extracts (LGE) on ulcerative colitis in children and adolescents. Methods Colitis was induced by DSS (Dextran Sulfate Sodium) in C57BL/6 mice. The sample mice were divided into group of four. The mice in the control group were not inflammation-induced. The control group was composed of untreated ulcerative colitis elicited mice. The mice in the experimental group were administered with Pentasa and another experimental group mice were treated with LGE after colitis elicitation. The effects on ulcerative colitis were evaluated by the morphological changes of colonic mucosa, decrease in the effect of pro-inflammatory cytokines (TNF-α and NF-κB) and inflammatory cytokines (iNOS and COX-2) in the mucosa. Results LGE showed protective effects in DSS induced ulcerative colitis. LGE inhibited shortening of colon length and relieved the hemorrhagic erosion in colonic mucosa. LGE decreased pro-inflammatory cytokines (TNF-α and NF-κB) and inflammatory cytokines (iNOS and COX-2). According to the GC/MS analysis, N-methyl pyrrolidone (NMP) was identified. Conclusions The result shows the clinical efficacy of LGE and demonstrates possible treatment options for ulcerative colitis. Further investigations for biological activity and chemical analysis of LGE will be needed.

Abstract

Objectives The purpose of this study is to investigate the anti-inflammatory effect of Lonicera Japonica-Glycyrrhiza Uralensis decoction extracts (LGE) on ulcerative colitis in children and adolescents. Methods Colitis was induced by DSS (Dextran Sulfate Sodium) in C57BL/6 mice. The sample mice were divided into group of four. The mice in the control group were not inflammation-induced. The control group was composed of untreated ulcerative colitis elicited mice. The mice in the experimental group were administered with Pentasa and another experimental group mice were treated with LGE after colitis elicitation. The effects on ulcerative colitis were evaluated by the morphological changes of colonic mucosa, decrease in the effect of pro-inflammatory cytokines (TNF-α and NF-κB) and inflammatory cytokines (iNOS and COX-2) in the mucosa. Results LGE showed protective effects in DSS induced ulcerative colitis. LGE inhibited shortening of colon length and relieved the hemorrhagic erosion in colonic mucosa. LGE decreased pro-inflammatory cytokines (TNF-α and NF-κB) and inflammatory cytokines (iNOS and COX-2). According to the GC/MS analysis, N-methyl pyrrolidone (NMP) was identified. Conclusions The result shows the clinical efficacy of LGE and demonstrates possible treatment options for ulcerative colitis. Further investigations for biological activity and chemical analysis of LGE will be needed.

참고문헌 (45)

  1. Xavier RJ, "Unravelling the pathogenesis of inflammatory bowel disease", Nature, 448(7152), pp.427~434 (2007)[DOI]
  2. Lee JH, "Inflammatory bowel disease in pediatric age", Korean J Pediatr Gastroenterol Nutr, 14(Suppl 1), pp.S34~S41 (2011)[DOI]
  3. Yang SK, "Incidence and prevalence of ulcerative colitis in the Songpa-Kangdong district, Seoul, Korea, 1986-1997", J Gastroenterol Hepatol, 15(9), pp.1037~1042 (2000)[DOI]
  4. Yang SK, "Epidemiology of inflammatory bowel disease in the Songpa-Kangdong district, Seoul, Korea, 1986-2005: a KASID study", Inflamm Bowel Dis, 14(4), pp.542~549 (2008)[DOI]
  5. Kim YM, "Clinical characteristics and long-term course of ulcerative colitis in Korea", Intest Res, 4(1), pp.12~21 (2006)
  6. Katz J, "The course of inflammatory bowel disease", Med Clin North Am, 78(6), pp.1275~1280 (1994)[DOI]
  7. 양동훈, "한국인에서 궤양성 대장염은 증가하는가?", 대한내과학회지, 76(6), pp.637~642 (2009), 대한내과학회
  8. Baldassano RN, "Inflammatory bowel disease in pediatric and adolescent patients", Gastroenterol Clin North Am, 28(2), pp.445~458 (1999)[DOI]
  9. Hickie I, "The temporal stability and co-morbidity of prolonged fatigue: a longitudinal study in primary care", Psychol Med, 29(4), pp.855~861 (1999)[DOI]
  10. 한예지, "소아 청소년의 과민성 장증후군이 식습관, 피로감에 미치는 영향에 관한 연구", 대한한방소아과학회지, 31(3), pp.1~13 (2017), 대한한방소아과학회
  11. 진윤태, "궤양성 대장염의 최신치료", 대한내과학회지, 76(6), pp.654~660 (2009), 대한내과학회
  12. 차호열, "금은화 추출물의 항산화, 항염증 효과가 Dextran Sulfate Sodium으로 유도된 생쥐의 궤양성 대장염에 미치는 영향", 대한한방소아과학회지, 29(3), pp.54~64 (2015), 대한한방소아과학회
  13. 김소연, "唐木香이 DSS(Dextran sulfate sodium)로 유발된 염증성 장질환 동물모델에 미치는 영향", 대한한방내과학회지, 34(2), pp.134~146 (2013), 대한한방내과학회
  14. Kim Y, "Research trends of Lonicera Japonica over the last 10 year", J Daejeon Orient Med, 19(1), pp.17~23 (2010)
  15. 주정석, "소회향과 금은화 추출물의 급성췌장염에 대한 예방효과", 대한본초학회지, 25(4), pp.39~45 (2010), 대한본초학회
  16. 안상현, "LPS 유도 생쥐 관절염에서 금은화의 MIF 활성 조절이 COX-2와 MMP-9 생성 억제에 미치는 영향", 동의생리병리학회지, 24(2), pp.242~248 (2010), 한의병리학회
  17. 박종필, "Suppression of Inflammatory Macrophage Responese by Glycyrrhiza Uralensis Herbal Acupuncture Extract", Korean Journal of Acupuncture, 28(4), pp.49~58 (2011), 경락경혈학회
  18. 박찬흠, "H2O2로 유도된 C6 신경교세포의 세포사멸에 대한 감초 추출물과 감초 활성물질의 보호효과", 한국약용작물학회지, 25(5), pp.315~321 (2017), 한국약용작물학회[DOI]
  19. Asl MN, "Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds", Phytother Res, 22(6), pp.709~724 (2008)[DOI]
  20. Kim BJ, "Characteristics and trends in the incidence of inflammatory bowel disease in Korean children: a single-center experience", Dig Dis Sci, 55(7), pp.1989~1995 (2010)[DOI]
  21. Itzkowitz SH, "Inflammation and cancer IV. Colorectal cancer in inflammatory bowel disease: the role of inflammation", Am J Physiol Gastrointest Liver Physiol, 287(1), pp.G7~G17 (2004)[DOI]
  22. Fazio V, "Current status of surgery for inflammatory bowel disease", Digestion, 59(5), pp.470~480 (1998)[DOI]
  23. Surh YJ, "Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation", Mutat Res, 480-481, pp.243~268 (2001)[DOI]
  24. Hendrickson BA, "Clinical aspects and pathophysiology of inflammatory bowel disease", Clin Microbiol Rev, 15(1), pp.79~94 (2002)[DOI]
  25. Lichtenstein GR, "Importance of mucosal healing in ulcerative colitis", Inflamm Bowel Dis, 16(2), pp.338~346 (2010)[DOI]
  26. Danese S, "Inflammatory bowel disease: the role of environmental factors", Autoimmun Rev, 3(5), pp.394~400 (2004)[DOI]
  27. Ishiguro K, "Paeonol attenuates TNBS-induced colitis by inhibiting NF- kappa B and STAT1 transactivation", Toxicol Appl Pharmacol, 217(1), pp.35~42 (2006)[DOI]
  28. 명노일, "Dextran sulfate sodium으로 유도된 궤양성 대장염에 대한 와송의 억제효과", 대한본초학회지, 29(4), pp.29~34 (2014), 대한본초학회
  29. 강용구, "금은화의 type Ⅰ interferon 억제효과 및 기전에 관한 연구", 대한한방부인과학회지, 26(2), pp.17~32 (2013), 대한한방부인과학회
  30. 유화승, "소염약침액이 Collagen II 유발 관절염 mouse의 TNF-α, IFN-γ 생성 및 비장세포 증식에 미치는 영향", Journal of Pharmacopuncture, 10(2), pp.31~40 (2007), 대한약침학회
  31. Wang ZY, "Licorice and cancer", Nutr Cancer, 39(1), pp.1~11 (2001)[DOI]
  32. 윤미영, "감초 추출물이 glutathione S-transferase의 유도 활성에 미치는 영향", Applied Biological Chemistry, 51(3), pp.228~232 (2008), 한국응용생명화학회
  33. Bian J, "N-methyl pyrrolidone promotes ankle fracture healing by inhibiting inflammation via suppression of the mitogen-activated protein kinase signaling pathway", Exp Ther Med, 15(4), pp.3617~3622 (2018)
  34. Ghayor C, "N-methyl pyrrolidone (NMP) inhibits lipopolysaccharide-induced inflammation by suppressing <TEX>$NF-{\kappa}B$<", TEX> signaling, 2015, pp.64(7), 527~536
  35. Gassler N, "Inflammatory bowel disease is associated with changes of enterocytic junctions", Am J Physiol Gastrointest Liver Physiol, 281(1), pp.216~228 (2001)[DOI]
  36. Melgar S, "Psychological stress reactivates dextran sulfate sodium-induced chronic colitis in mice", Stress, 11(5), pp.348~362 (2008)[DOI]
  37. Xie QW, "Role of transcription factor NF-kappa B", Rel in induction of nitric oxide synthase, 1994, pp.269(7), 4705~4708
  38. 최준용, "항염증조절을 통한 자금정의 DSS 유도 궤양성 대장염 완화 효과", 대한한방내과학회지, 38(6), pp.944~954 (2017), 대한한방내과학회
  39. Groszmann RJ, "Hyperdynamic state in chronic liver diseases", J Hepatol, 17(2), pp.38~40 (1993)[DOI]
  40. RothkOtter HJ, "Lymphocyte migration in the intestinal mucosa: entry, transit and emigration of lymphoid cells and the influence of antigen", Vet Immunol Immunopathol, 72(1-2), pp.157~165 (1999)[DOI]
  41. Barnes PJ, "Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases", N Engl J Med, 336(15), pp.1066~1071 (1997)[DOI]
  42. Baeuerle PA, "<TEX>$I{\kappa}B-NF-{\kappa}B$<", TEX> structures: at the interface of inflammation control, 1998, pp.95(6), 729~731
  43. 최창환, "Second Korean guidelines for the management of ulcerative colitis", Intestinal research, 15(1), pp.7~37 (2017), 대한장연구학회
  44. 이광재, "염증성 장질환의 약물요법", 대한의사협회지, 58(1), pp.57~63 (2015), 대한의사협회[DOI]
  45. 박병준, "관해기 궤양성 대장염의 재발률 및 재발 관련 인자들", 대한소화기학회지, 52(1), pp.21~26 (2008), 대한소화기학회

관련 논문AI추천

금은화 추출물의 항산화, 항염증 효과가 Dextran Sulfate Sodium으로 유도된 생쥐의 궤양성 대장염에 미치는 영향김기봉, 박선영, 안상현, 정아람, 차호열, 천진홍대한한방소아과학회 · 대한한방소아과학회지2015 · 제29권 제3호항염증조절을 통한 자금정의 DSS 유도 궤양성 대장염 완화 효과김기봉, 사복석, 사은호, 안상현, 최준용대한한방내과학회 · 대한한방내과학회지2017 · 제38권 제6호궤양성 대장염 유발 생쥐에 투여한 계지가용골모려탕의 치료효과강아미, 임성우대한한방내과학회 · 대한한방내과학회지2017 · 제38권 제6호槐花散이 Dextran Sulfate Sodium으로 유도된 생쥐의 궤양성 대장염에 미치는 영향공경환, 배광호대한한방내과학회 · 대한한방내과학회지2010 · 제31권 제3호消炎整腸湯이 DSS로 誘發된 생쥐의 궤양성 대장염에 미치는 影響류봉하, 송영근, 윤성우대한한방내과학회 · 대한한방내과학회지2008 · 제29권 제2호염증성 장 질환 동물 모델에서 황금탕과 감초사심탕의 효능 연구김영광, 문영호대한한방내과학회 · 대한한방내과학회지2021 · 제42권 제3호

이 간행물의 다른 논문 (10건)

택사 (澤瀉, Alismatis Rhizoma) 추출물이 RBL-2H3 비만세포와 OVA/alum 감작 생쥐의 알레르기 염증 반응에 미치는 영향이진화, 송지현, 김은진, 김윤희pp. 1-15중의학 데이터베이스 (CAJ)를 이용한 소아 비만의 한약치료에 대한 임상 연구 동향류향, 천진홍, 김기봉, 김서형pp. 26-43비부비동염의 보완대체의학 약물치료에 대한 최신 임상 연구 동향- RCT 연구를 중심으로 -장은하, 김장현, 민상연pp. 44-54한의사 교의 사업에 대한 학부모의 만족도 조사 연구이승환, 고호연, 성현경, 박정수, 김동수, 신선미pp. 55-61한의사 교의 사업을 통한 소아청소년기 금연 교육의 효용성 연구성현경, 고호연, 박장경, 김동수, 신선미, 박정수, 이승환pp. 62-71
원문 보기