Genoprotective Effect of Melatonin Against to the Genotoxicity of Glyphosate on Human Blood Lymphocytes
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Purpose: Glyphosate is a widely used non-selective herbicide. Previous studies have shown that glyphosate has genotoxicity, and that even low-doses of glyphosate can cause DNA damage. Melatonin is a hormone produced and secreted by the pineal gland that is known to be a potent anti-carcinogen, anti-oxidant, and genetic protector. This study was conducted to investigate the genoprotective effect of melatonin against glyphosate in human blood lymphocytes. Methods: Human peripheral blood was obtained from 15 young, healthy volunteers and cultured under four different toxicologic conditions. The four groups consisted of a control group, glyphosate only group (300 ng/mL), glyphosate with low level of melatonin group (50 μM), and glyphosate with high level of melatonin group (200 μM). The mean Sister Chromatid Exchange (SCE) frequency of each group was then analyzed. Results: Glyphosate exposed groups had a higher mean SCE frequency (10.33±2.50) than the control group (6.78 ±2.31, p<0.001). Interestingly, the group that received a low-level of melatonin had a lower mean SCE frequency (8.67±2.58) than the glyphosate-only group, while the group that received a high level of melatonin had a much lower mean SCE frequency (8.06±2.50) than the glyphosate-only group. There was statistical significance. Conclusion: Melatonin exerted a potent gene protective effect against the genotoxicity of glyphosate on human blood lymphocytes in a dose-dependent fashion.
Abstract
Purpose: Glyphosate is a widely used non-selective herbicide. Previous studies have shown that glyphosate has genotoxicity, and that even low-doses of glyphosate can cause DNA damage. Melatonin is a hormone produced and secreted by the pineal gland that is known to be a potent anti-carcinogen, anti-oxidant, and genetic protector. This study was conducted to investigate the genoprotective effect of melatonin against glyphosate in human blood lymphocytes. Methods: Human peripheral blood was obtained from 15 young, healthy volunteers and cultured under four different toxicologic conditions. The four groups consisted of a control group, glyphosate only group (300 ng/mL), glyphosate with low level of melatonin group (50 μM), and glyphosate with high level of melatonin group (200 μM). The mean Sister Chromatid Exchange (SCE) frequency of each group was then analyzed. Results: Glyphosate exposed groups had a higher mean SCE frequency (10.33±2.50) than the control group (6.78 ±2.31, p<0.001). Interestingly, the group that received a low-level of melatonin had a lower mean SCE frequency (8.67±2.58) than the glyphosate-only group, while the group that received a high level of melatonin had a much lower mean SCE frequency (8.06±2.50) than the glyphosate-only group. There was statistical significance. Conclusion: Melatonin exerted a potent gene protective effect against the genotoxicity of glyphosate on human blood lymphocytes in a dose-dependent fashion.
참고문헌 (30)
- Williams GM, "Safety evaluation and risk assessment of the herbicide Roundup and its active ingredient, glyphosate, for humans", Regul Toxicol Pharmacol, 31, pp.117~165 (2000)
- Talbot AR, "Acute poisoning with a glyphosate-surfactant herbicide (‘Roundup’): a review of 93 cases", Hum Exp Toxicol, 10, pp.1~8 (1991)
- Richard S, "Differential effects of glyphosate and roundup on human placental cells and aromatase", Environ Health Perspect, 113, pp.716~720 (2005)
- Koller VJ, "Cytotoxic and DNA-damaging properties of glyphosate and Roundup in humanderived buccal epithelial cells", Arch Toxicol, 86, pp.805~813 (2012)
- Lee SH, "Genotoxicity of low-dose Glyphosate by Sister Chromatid Exchange", J Korean Soc Clin Toxicol, 12, pp.8~13 (2014)
- De Roos AJ, "Cancer incidence among glyphosateexposed pesticide applicators in the Agricultural Health Study", Environ Health Perspect, 113, pp.49~54 (2005)
- Bolognesi C, "Biomonitoring of genotoxic risk in agricultural workers from five colombian regions: association to occupational exposure to glyphosate", J Toxicol Environ Health A, 72, pp.986~997 (2009)
- Schinasi L, "Non-Hodgkin lymphoma and occupational exposure to agricultural pesticide chemical groups and active ingredients: a systematic review and metaanalysis", Int J Environ Res Public Health, 11, pp.4449~4527 (2014)
- Reiter RJ, "The melatonin rhythm: both a clock and a calendar", Experientia, 49, pp.654~664 (1993)
- Hardeland R, "Melatonin", Int J Biochem Cell Biol, 38, pp.313~316 (2006)
- Barlow-Walden LR, "Melatonin stimulates brain glutathione peroxidase activity", Neurochem Int, 26, pp.497~502 (1995)
- Tan D, "Chemical and physical properties and potential mechanisms: melatonin as a broad spectrum antioxidant and free radical scavenger", Curr Top Med Chem, 2, pp.181~197 (2002)
- Cos S, "Melatonin and mammary pathological growth", ront Neuroendocrinol, 21, pp.133~170 (2000)
- anthracene-induced carcinogenesis of the uterine cervix and vagina in mice and mutagenesis in vitro, "Cancer Lett", 2000, 199~205
- Anisimov VN, "The Pineal Gland and Cancer", pp.240~258 (2001), Springer
- Cos S, "Mediavilla M. Melatonin and mammary cancer: a short review", Endocr Relat Cancer, 10, pp.153~159 (2003)
- Subramanian P, "Prevention by melatonin of hepatocarcinogenesis in rats injected with Nnitrosodiethylamine", J Pineal Res, 43, pp.305~312 (2007)
- Mills E, "Melatonin in the treatment of cancer: a systematic review of randomized controlled trials and meta-analysis", J Pineal Res, 39, pp.360~366 (2005)
- Vijayalaxmi, "Melatonin reduces gamma radiation-induced primary DNA damage in human blood lymphocytes", Mutat Res, 397, pp.203~208 (1998)
- Lee JH, "In vitro and in vivo effects of melatonin on sister chromatid exchange in human blood lymphocytes exposed to hypoxia", DrugChem Toxicol, pp.1~4 (2015)
- Anisimov VN, "Melatonin as antioxidant, geroprotector and anticarcinogen", Biochim Biophys Acta, 1757, pp.573~589 (2006)
- Sarto F, "Aging and smoking increase the frequency of sister-chromatid exchanges (SCE) in man", Mutat Res, 144, pp.183~187 (1985)
- Hirsch BA, "Genetic and environmental influences on baseline SCE", Environ Mol Mutagen, 20, pp.2~11 (1992)
- Wilson DM, 3rd, "Molecular mechanisms of sister-chromatid exchange", Mutat Res, 616, pp.11~23 (2007)
- Stults DM, "The sister chromatid exchange (SCE) assay", Methods Mol Biol, 1105, pp.439~455 (2014)
- Tucker JD, "Sister-chromatid exchange: second report of the Gene-Tox Program", Mutat Res, 297, pp.101~180 (1993)
- Hagmar L, "Chromosomal aberrations in lymphocytes predict human cancer: a report from the European Study Group on Cytogenetic Biomarkers and Health (ESCH)", Cancer Res, 58, pp.4117~4121 (1998)
- Mourelatos D, "Sister chromatid exchange assay as a predictor of tumor chemoresponse", Mutat Res Genet Toxicol Environ Mutagen, 803-804, pp.1~12 (2016)
- Galley HF, "Melatonin as a potential therapy for sepsis: a phase I dose escalation study and an ex vivo whole blood model under conditions of sepsis", J Pineal Res, 56, pp.427~438 (2014)
- Shokouhi G, "Neuroprotective effects of high-dose vs low-dose melatonin after blunt sciatic nerve injury", Childs Nerv Syst, 24, pp.111~117 (2008)