Spirulina maxima 초고압 추출물의 피부 면역 활성 증진
Enhancement of Skin Immune Activities of Spirulina maxima by High Pressure Extraction Process
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초록
A marine alga, Spirulina maxima, was extracted under high pressure and low temperature conditions at 500 MPa and 60oC for 5 and 10 min. A high pressure of 500 MPa was applied to improve process yields because of low temperature extraction. This method resulted in highest higher extraction yield of 26.1% (w/w) in comparison to those results obtained from conventional extraction methods which produced a yield of 17.6% (w/w) from water. The extracts from this process also showed 19% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. The crude extract significantly reduced the production of Prostaglandin E2 (PGE2) from CCD-986sk cells and increased nitric oxide production by macrophages. These higher activities of enhancing skin immune functions were found to have high antioxidant extract properties, like a 98% increase in DPPH radical scavenging activity. The extracts from the high pressure process showed a higher elution of active components than other processes and generated new compounds based on HPLC analysis. This clearly indicates that the extracts from high pressure and low temperature conditions have higher skin immune activation properties that have not been previously reported.
Abstract
A marine alga, Spirulina maxima, was extracted under high pressure and low temperature conditions at 500 MPa and 60oC for 5 and 10 min. A high pressure of 500 MPa was applied to improve process yields because of low temperature extraction. This method resulted in highest higher extraction yield of 26.1% (w/w) in comparison to those results obtained from conventional extraction methods which produced a yield of 17.6% (w/w) from water. The extracts from this process also showed 19% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. The crude extract significantly reduced the production of Prostaglandin E2 (PGE2) from CCD-986sk cells and increased nitric oxide production by macrophages. These higher activities of enhancing skin immune functions were found to have high antioxidant extract properties, like a 98% increase in DPPH radical scavenging activity. The extracts from the high pressure process showed a higher elution of active components than other processes and generated new compounds based on HPLC analysis. This clearly indicates that the extracts from high pressure and low temperature conditions have higher skin immune activation properties that have not been previously reported.
참고문헌 (25)
- 강민숙, "기능성 생물 소재로서의 클로렐라", KSBB Journal, 19(1), pp.1~11 (2004), 한국생물공학회
- 이윤진, "스피루리나를 첨가한 생면과 조리면의 저장 중 품질특성", 한국식품저장유통학회지, 16(1), pp.23~32 (2009), 한국식품저장유통학회
- Bernstein EF, "Enhanced elastin and fibrillin gene expression in chronically photodamaged skin", J Invest Dermatol, 103(2), pp.182~186 (1994)
- Choi SY, "Synthesis, discovery and mechanism of 2,6-dimethoxy-N-(4-methoxyphenyl)benzamide as potent depigmenting agent in the skin", Biochem Bioph Res Co, 349(1), pp.39~49 (2006)
- Chung TY, "Antioxidative activity of flavonoids isolated from jindalrae flower", Plant Physiol Bioch, 39(4), pp.320~326 (1996)
- Diego JM, "Tocopherol measurement in edible products of vegetable origin", J Chromatogr A, 1054(1), pp.227~233 (2004)
- Grinstead GS, "Effects of Spifulina Platensis in growth performance of weanling pigs", Anim Feed Sci Tech, 83(3), pp.237~247 (2000)
- Hernandez AC, "Antiviral activity of Spirulina maxima against herpes simplex virus type 2", Antivir Res, 56(3), pp.297~285 (2002)
- Jeong DM, "Antioxidative treatment method by lxeris dentata for conquer cancer and stress", J Korean Soc Jungshin Sci, 6(1), pp.69~82 (2002)
- 정향숙, "불가사리 유래 콜라겐 펩타이드의 피부 면역 증진 효과", 한국식품과학회지, 40(5), pp.522~527 (2008), 한국식품과학회
- 정명훈, "고로쇠 수피 초고압 추출물의 항암활성 증진", 한국식품영양과학회지, 38(9), pp.1243~1252 (2009), 한국식품영양과학회
- 정명훈, "고로쇠와 우산고로쇠 저온고압 수용성 추출물의 유용생리활성 비교", 한국약용작물학회지, 17(6), pp.407~414 (2009), 한국약용작물학회
- 김영, "저온고압 추출공정을 이용한 매자나무 수피의 항산화 및 항암활성 증진", 한국식품과학회지, 41(3), pp.284~291 (2009), 한국식품과학회
- Jose C, "Free radical-scavenging, antioxidant and immunostimulating effects of a licorice infusion", Food Chem., 122(3), pp.508~517 (2010)
- 정성제, "약용 식물 추출물의 항산화 활성 검색", Journal of the Korean Society for Applied Biological Chemistry, 47(1), pp.135~140 (2004), 한국응용생명화학회
- Kaji T, "Repair of wounded monolayers of cultured bovine aortic endothelial cells is inhibited by calcium spirulan, a novel sulfated polysaccharide isolated form Spirulina platensis", Life Sci, 70(16), pp.1841~1848 (2002)
- Kay RA, "Microalgae as food and supplement", Crit Rev Food Sci, 30(6), pp.555~573 (1991)
- Oh SH, "The effect of ultrasonificated extracts of Spirulina maxima on the anticancer activity", Mar Biotechnol. (2010)
- 이현화, "흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교", 한국약용작물학회지, 16(2), pp.79~85 (2008), 한국약용작물학회
- Lee HS, "Screening of the immuno-stimulatory activity of the marine alga Chlorella capsulate", Kor J Biotechnol Bioeng, 18(1), pp.19~24 (2003)
- Liou JY, "Cyclooxygenase-2-derived prostaglandin e2 protects mouse embryonic stem cells from apoptosis", Stem Cells, 25(5), pp.1096~1103 (2007)
- Mosmann T, "Rapid colorimetric assay for the cellular growth and survival: Application to proliferation and cytotoxic assay", J Immunol Methods, 65(1), pp.55~63 (1983)
- Nandeesha MC, "Growth performance of two indian major carps, catla fed diets containing different levels of Spirulina platensis", Bioresour Technol, 80(2), pp.117~120 (2001)
- Park JH, "Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages", Biochem Biophys Res Commun, 351(1), pp.146~152 (2006)
- Yang HN, "Spirulina platensis inhibits anaphylactic reactin", Life Sci, 61(13), pp.1237~1244 (1997)