BaP 및 TBT에 노출된 넙치와 개조개의 in vivo Comet assay
In vivo Comet Assay on Flounder and Clam Exposed to BaP and TBT
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참고문헌
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초록
The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide (H2O2); strong correlation between H2O2 concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1,10, 50, 100 μg/L) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.
Abstract
The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide (H2O2); strong correlation between H2O2 concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1,10, 50, 100 μg/L) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.
참고문헌 (36)
- 김기범, "Pyrene과 Benzo(a)pyrene에 노출된 굴의 혈구세포와 아가미 세포에서의 DNA 손상 측정을 위한 Comet assay의 이용", 한국양식학회지, 16(3), pp.196~201 (2003), 한국양식학회
- 서진영, "Comet assay를 이용한 갯지렁이의 혈구세포에 대 한 유전독성 평가", J Environ Toxicol, 20(4), pp.331~341 (2005)
- Alzieu C, "Environmental impact of TBT: the French experience", Sci Total Environ, 258, pp.99~102 (2000)
- Binelli A, "Assessment of the genotoxic potential of benzo (a) pyrene and pp'- dichlorodiphenyldichloroethylene in Zebra mussel (Dreissena polymorpha)", Mutat Res Gen Tox En, 649, pp.135~145 (2008)
- Birmelin C, "Characterisation of biotransformation enzyme activities and DNA integrity in isolated cells of the digestive gland of the common mussel, Mytilus edulis L", Comp Biochem Phys A, 120, pp.51~56 (1998)
- Collins A, "Comet assay in human biomonitoring studies: reliability, validation, and applications", Environ Mol Mutagen, 30, pp.139~146 (1997), 139-146
- Coughlan B, "Detecting genotoxicity using the Comet assay following chronic exposure of Manila clam Tapes semidecussatus to polluted estuarine sediments", Mar Pollut Bull, 44, pp.1359~1365 (2002)
- Daughton CG, "Pharmaceuticals and personal care products in the environment: agents of subtle change", Environ Health Persp, 107(6), pp.907~938 (1999)
- Fleeger JW, "Indirect effects of contaminants in aquatic ecosystems", Sci Total Environ, 317, pp.207~233 (2003)
- pyrene on the immune-associated activities of hemocytes and recovery responses in the gastropod abalone, Haliotis diversicolor, "Comp Biochem Phys C", 2011, 120~128
- Guillette LJ, "Environmental contaminants and reproductive abnormalities in wildlife: implications for public health", Toxicology and Industrial Health, 12, pp.537~550 (1996)
- Jha AN, "Impact of low doses of tritium on the marine mussel, Mytilus edulis: Genotoxic effects and tissue-specific bioconcentration", Mutat Res Gen Tox En, 586, pp.47~57 (2005)
- Kummerer K, "Drugs in the environment: emission of drugs, diagnostic aids and disinfectants into wastewater by hospitals in relation to other sources-a review", Chemosphere, 45, pp.957~969 (2001)
- La Rocca N, "Survey on a microalga collected from an Edmonson Point pond (Victoria Land, Antarctica)", Plant Biosyst, 130, pp.960~962 (1996)
- 이정석, "Acute Toxicity of Heavy Metals, Tributyltin, Ammonia and Polycyclic Aromatic Hydrocarbons to Benthic Amphipod Grandidierella japonica", Ocean Science Journal, 40(2), pp.61~66 (2005), 한국해양연구원
- Lee RF, "Use of the single cell gel electrophoresis", comet assay for detecting DNA damage in aquatic (marine and freshwater) animals, 2003, pp.544, 43~64
- Ma T, "Biomarker responses and reproductive toxicity of the effluent from a Chinese large sewage treatment plant in Japanese medaka (Oryzias latipes)", Chemosphere, 59, pp.281~288 (2005)
- Michel P, "Tributyltin along the coasts of Corsica (Western Mediterranean): A persistent problem", Mar Pollut Bull, 42, pp.1128~1132 (2001)
- Mitchelmore C, "DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring", Mutat Res Fund Mol M, 399, pp.135~147 (1998)
- Mitchelmore CL, "Assessing DNA damage in cnidarians using the Comet assay", Mar Environ Res, 58, pp.707~711 (2004)
- Paul J, "Effects of copper-and tin-based antifouling compounds on the growth of scallops (Pecten maximus) and oysters (Crassostrea gigas)", Aquaculture, 54, pp.191~203 (1986)
- Petridis P, "Measurements of the genotoxic potential of (xeno-) oestrogens in the bivalve mollusc Scrobicularia plana, using the Comet assay", Aquat Toxicol, 94, pp.8~15 (2009)
- Pointet K, "PAHs analysis of fish whole gall bladders and livers from the Natural Reserve of Camargue by GC", MS, 2000, pp.40, 293~299
- Pruski AM, "Effects of cadmium on nuclear integrity and DNA repair efficiency in the gill cells of Mytilus edulis L", Aquat Toxicol, 57, pp.127~137 (2002)
- Santos M, "Cytochrome P450 differences in normal and imposex-affected female whelk Buccinum undatum from the open North Sea", Mar Environ Res, 54, pp.661~665 (2002)
- Schiff K, "Impacts of stormwater discharges on the nearshore benthic environment of Santa Monica Bay", Mar Environ Res, 56, pp.225~243 (2003)
- 김승규, "Imposex in the rock shell, Thais clavigera, as evidence of organotin contamination in the marine environment of Korea", MARINE ENVIRONMENTAL RESEARCH, 49(5), pp.435~451
- Singh NP, "A simple technique for quantitation of low levels of DNA damage in individual cells", Exp Cell Res, 175, pp.184~191 (1988)
- Steinert SA, "DNA damage in mussels at sites in San Diego Bay", Mutat Res Fund Mol M, 399, pp.65~85 (1998)
- Tice R, "Single cell gel", comet assay: guidelines for in vitro and in vivo genetic toxicology testing, 2000, pp.35, 206~221[DOI]
- Tice R, "Protocol for the application of the pH> 13 alkaline single cell gel (SCG) assay to the detection of DNA damage in mammalian cells", ~ (1998)
- comet'assay) in relation to the induction of sister-chromatid exchanges and micronuclei, "Toxicology", 1998, 129~139
- - ethinylestradiol and endosulfan on Crassostrea gigas embryos, "Aquat Toxicol", 2007, 133~142
- Woo S, "Comet assay for the detection of genotoxicity in blood cells of flounder (Paralichthys olivaceus) exposed to sediments and polycyclic aromatic hydrocarbons", MARINE POLLUTION BULLETIN, 52, pp.1768~1775 (2006), PERGAMON-ELSEVIER SCIENCE LTD
- pyrene, tributyltin and their mixture in the spleen of Sebasticus marmoratus, "J Environ Sci", 2007, 1129~1135
- Zhang J, "Effect of tributyltin on the development of ovary in female cuvier (Sebastiscus marmoratus)", Aquat Toxicol, 83, pp.174~179 (2007)