과산화아질산염과 DPPH 라디칼에 대한 해조 추출물의 소거 활성 효과
Studies on Screening of Seaweed Extracts for Peroxynitrite and DPPH Radical Scavenging Activities
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Abstract
As a part of our search for novel antioxidants from the seaweeds, we have investigated radical scavenging effect for their crude extracts using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1), a peroxynitrite-generating species in vitro. Thirty-four seaweeds were screened for ONOO- and DPPH radical scavenging activities. A potent inhibitory effect against peroxynitrite generated by SIN-1 at 5 mg/ml of methanol extracts was observed in order of Ishige okamurae (95.3%), Sargassum hemiphyllum (90.2%), Symphyocladia latiuscula (89.6%), Porphyra suborbiculata (86.7%), and Gelidium amansii (85.9%). Also, a significant scavenging effect against direct authentic peroxynitrite was revealed for methanol extracts of Ishige okamurae (66.2%) and Sargassum hemiphyllum (55.2%) and the acetone/methylene chloride (1:1) extract of Gigatina tenella (61.0%). In our measurement for evaluating the capacity to scavenge the stable free radical of DPPH, acetone/methylene chloride (1:1) extracts of Symphyocladia latiuscula, Gloiopeltis furcata, and Sargassum thunbergii and the methanol extract of Sargassum sp. showed an inhibitory potency of 85.8%, 82.8%, 74.1%, and 64.0%, respectively.
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