해양 규조류 Cyclotella meneghiniana의 성장단계에 따른 비타민(B1, B7, B12) 및 특이적 미생물의 변동
Changes in Vitamins (B1, B7, and B12) and Specific Bacteria on the Growth Stages of Marine Diatom Cyclotella meneghiniana
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Diatom growth is affected by associated bacteria that probably provide useful substances like vitamins. In the present study, we analysed the variation of vitamins B1, B7 and B12 on the growth stages of the marine diatom Cyclotella meneghiniana and assessed putative vitamin-producing bacteria (e.g., α- and γ-proteobacteria). HPLC analysis showed that total amounts of vitamins B1 and B12 decreased with cell growth, whereas vitamin B7 increased gradually on the growth stages. B1 and B12 measured 0.5% and 0.18% at the stationary phase, following 0.25% and 0.72% at the lag phase. They considerably increased to 0.75% and 0.77% at the death stage. 16S pyrosequencing showed relatively high ratios of α- and γ- proteobacteria in all the growth stages of the C. meneghiniana. In addition, we detected previously-reported vitamin-producing bacteria, such as Marinobacter, in high numbers. The species was dorminant in the lag (relative abundance 72%) and exponetial (72%) stages, whareas it decreased in the stationary (49%) and death (48%) stages. These results suggest that vitamins B1 or B12 may be necesaary for diatom growth and that associated bacteria, including Marinobacter, may produce these substances for the cell growth of C. meneghiniana.
Abstract
Diatom growth is affected by associated bacteria that probably provide useful substances like vitamins. In the present study, we analysed the variation of vitamins B1, B7 and B12 on the growth stages of the marine diatom Cyclotella meneghiniana and assessed putative vitamin-producing bacteria (e.g., α- and γ-proteobacteria). HPLC analysis showed that total amounts of vitamins B1 and B12 decreased with cell growth, whereas vitamin B7 increased gradually on the growth stages. B1 and B12 measured 0.5% and 0.18% at the stationary phase, following 0.25% and 0.72% at the lag phase. They considerably increased to 0.75% and 0.77% at the death stage. 16S pyrosequencing showed relatively high ratios of α- and γ- proteobacteria in all the growth stages of the C. meneghiniana. In addition, we detected previously-reported vitamin-producing bacteria, such as Marinobacter, in high numbers. The species was dorminant in the lag (relative abundance 72%) and exponetial (72%) stages, whareas it decreased in the stationary (49%) and death (48%) stages. These results suggest that vitamins B1 or B12 may be necesaary for diatom growth and that associated bacteria, including Marinobacter, may produce these substances for the cell growth of C. meneghiniana.
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