부산 영도 내만에서 원핵생물 성장 및 다양성의 주간 변동 특성
Weekly Variation of Prokaryotic Growth and Diversity in the Inner Bay of Yeong-do, Busan
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
To understand the temporal variation of prokaryotic communities in a temperate coastal area, prokaryotic abundance, activity, and community composition were investigated every week for over a year at a coastal monitoring station of Yeong-do, Busan. The prokaryotic abundances fluctuated about 10 times, ranging from 2.0 to 20.1 × 105 cells mL-1 and tended to be high in spring when phytoplankton bloom occurred. The prokaryotic thymidine incorporation rates (TTI) varied in a low range between 0.2 and 11.5 pmol L-1 h-1 in winter. However, in summer, TTI were increased up to a range of 8.3 to 17.4 pmol L-1 h-1, showing an increasing pattern in summer. During the study period, Alphaproteobacteria was the most dominant class for most of the year, followed by Flavobacteria. While the seasonal variation of prokaryotic composition was not apparent at the class level, many prokaryotic species showed a distinct temporal or seasonal variation for the year. In the coastal site, prokaryotic biomass and activity did not show significant correlations with temperature and chlorophyll-a, which are well known to regulate prokaryotic growth in marine environments, suggesting that the study area may be affected by diverse sources of organic matter for their growth.
Abstract
To understand the temporal variation of prokaryotic communities in a temperate coastal area, prokaryotic abundance, activity, and community composition were investigated every week for over a year at a coastal monitoring station of Yeong-do, Busan. The prokaryotic abundances fluctuated about 10 times, ranging from 2.0 to 20.1 × 105 cells mL-1 and tended to be high in spring when phytoplankton bloom occurred. The prokaryotic thymidine incorporation rates (TTI) varied in a low range between 0.2 and 11.5 pmol L-1 h-1 in winter. However, in summer, TTI were increased up to a range of 8.3 to 17.4 pmol L-1 h-1, showing an increasing pattern in summer. During the study period, Alphaproteobacteria was the most dominant class for most of the year, followed by Flavobacteria. While the seasonal variation of prokaryotic composition was not apparent at the class level, many prokaryotic species showed a distinct temporal or seasonal variation for the year. In the coastal site, prokaryotic biomass and activity did not show significant correlations with temperature and chlorophyll-a, which are well known to regulate prokaryotic growth in marine environments, suggesting that the study area may be affected by diverse sources of organic matter for their growth.
참고문헌 (43)
- Alonso C, "Roseobacter and SAR11 dominate microbial glucose uptake in coastal North Sea waters", Environ Microbiol, 8, pp.2022~2030 (2006)
- Alonso-Sáez L, "Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH", FEMS Microbiol Ecol, 60, pp.98~112 (2007)
- Arrigo KR, "Marine microorganisms and global nutrient cycles", Nature, 437, pp.349~355 (2005)
- Azam F, "The ecological role of water-column microbes in the sea", Mar Ecol-Prog Ser, 10(3), pp.257~263 (1983)
- Belkin IM, "Rapid warming of large marine ecosystems", Prog Oceanogr, 81(1–4), pp.207~213 (2009)
- Brinkmeyer R, "Diversity and structure of bacterial communities in Arctic versus Antarctic pack ice", Appl Environ Microb, 69, pp.6610~6619 (2003)
- Bunse C, "Spatio-temporal interdependence of bacteria and phytoplankton during a Baltic Sea spring bloom", Front Microbiol, 7, pp.517~ (2016)[DOI]
- Campbell BJ, "Bacterial diversity, community structure and potential growth rates along an estuarine salinity gradient", ISME J, 7, pp.210~220 (2013)
- Choi DH, "Daily variation in the prokaryotic community during a spring bloom in shelf waters of the East China Sea", FEMS Microbiol Ecol, 94(fiy134) (2018)[DOI]
- Choi DH, "Different interrelationships among phytoplankton, bacterial and environmental variables in dumping and reference areas in the East Sea", Aquat Microb Ecol, 41, pp.171~180 (2005)
- Crossland CJ, "Coastal fluxes in the Anthropocene: the land-ocean interactions in the coastal zone project of the International Geosphere-Biosphere Programme", pp.232~ (2005), Springer
- Ducklow HW, "Bacterial dynamics and distribution during a spring diatom bloom in the Hudson Riv er plume, USA", J Plankton Res, 5, pp.333~355 (1983)
- Falkowski PG, "Biogeochemical controls and feedbacks on ocean primary production", Science, 281, pp.200~206 (1998)
- Fuller NJ, "Molecular analysis of photosynthetic picoeukaryote community structure along an Arabian Sea transect", Limnol Oceanogr, 51, pp.2502~2514 (2006)
- Gasol JM, "Towards a better understanding of microbial carbon flux in the sea", Aquat Microb Ecol, 53, pp.21~38 (2008)
- Giebel H-A, "Complementary energy acquisition via aerobic anoxygenic photosynthesis and carbon monoxide oxidation by Planktomarina temperata of the Roseobacter group", FEMS Microbiol Ecol, 95(fiz050) (2019)[DOI]
- Gilbert JA, "The seasonal structure of microbial communities in the Western English Channel", Environ Microbiol, 11, pp.3132~3139 (2009)
- Giovannoni SJ, "SAR11 Bacteria: the most abundant plankton in the oceans", Annu Rev Mar Sci, 9, pp.231~255 (2017)
- González JM, "Bacterial community structure associated with a dimethylsulfoniopropio-nate-producing North Atlantic algal bloom", Appl Environ Microb, 66, pp.4237~4246 (2000)
- Hyun J-H, "Bacterial abundance and production during the unique spring phytoplankton bloom in the central Yellow Sea", Mar Ecol-Prog Ser, 252, pp.77~88 (2003)
- "16S Metagenomic Sequencing Library Preparation" (2013)[URL]
- Kim DY, "Monthly to interannual variability of microbial eukaryote assemblages at four depths in the eastern North Pacific", ISME J, 8, pp.515~530 (2014)
- Kim J, "Inputs of humic fluorescent dissolved organic matter via submarine groundwater discharge to coastal waters off a volcanic island (Jeju, Korea)", Sci Rep, 7, pp.7921~ (2017)
- Kozich JJ, "Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform", Appl Environ Microb, 79, pp.5112~5120 (2013)
- Lindh MV, "Disentangling seasonal bacterioplankton population dynamics by high‐frequency sampling", Environ Microbiol, 17, pp.2459~2476 (2015)
- Marie D, "Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I", Appl Environ Microb, 63, pp.186~193 (1997)
- Mary I, "Seasonal dynamics of bacterioplankton community structure at a coastal station in the western English Channel", Aquat Microb Ecol, 42, pp.119~126 (2006)
- Monticelli L, "Role of prokaryotic biomasses and activities in carbon and phosphorus cycles at a coastal, thermohaline front and in offshore waters (Gulf of Manfredonia, Southern Adriatic Sea)", Microb Ecol, 67, pp.501~519 (2014)
- Needham DM, "Pronounced daily succession of phytoplankton, archaea and bacteria following a spring bloom", Nat Microbiol, 1, pp.1~7 (2016)
- Oh YH, "Importance of dissolved organic carbon flux through submarine groundwater discharge to the coastal ocean: results from Masan Bay, the southern coast of Korea", J Marine Syst, 173, pp.43~48 (2017)
- Parsons T, "A manual of chemical and biological methods for seawater analysis", pp.173~ (1984), Pergamon Press
- Pernthaler J, "Seasonal community and population dynamics of pelagic bacteria and archaea in a high mountain lake", Appl Environ Microb, 64, pp.4299~4306 (1998)
- Schloss PD, "Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities", Appl Environ Microb, 75, pp.7537~7541 (2009)
- Selje N, "A newly discovered Roseobacter cluster in temperate and polar oceans", Nature, 427, pp.445~448 (2004)
- Shiah F-K, "Temperature and substrate regulation of bacterial abundance, production and specific growth rate in Chesapeake Bay, USA", Mar Ecol-Prog Ser, 103, pp.297~380 (1994)
- Somerville C, "Simple, rapid method for direct isolation of nucleic acids from aquatic environments", Appl Environ Microb, 55, pp.548~554 (1989)
- Straza TR, "Single-cell response of bacterial groups to light and other environmental factors in the Delaware Bay, USA", Aquat Microb Ecol, 62, pp.267~277 (2011)
- Sturluson M, "Bacterial abundance, biomass and production during spring blooms in the northern Barents Sea", Deep-Sea Res Pt II, 55, pp.2186~2198 (2008)
- Suzuki MT, "Quantitative mapping of bacterioplankton populations in seawater: field tests across an upwelling plume in Monterey Bay", Aquat Microb Ecol, 24, pp.117~127 (2001)
- Takahashi S, "Development of a prokaryotic universal primer for simultaneous analysis of Bacteria and Archaea using next-generation sequencing", PloS One, 9, pp.e105592~ (2014)
- Teeling H, "Substrate-controlled succession of marine bacterioplankton populations induced by a phytoplankton bloom", Science, 336, pp.608~611 (2012)
- Wang Q, "Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy", Appl Environ Microb, 73, pp.5261~5267 (2007)
- White PA, "The effect of temperature and algal biomass on bacterial production and specific growth rate in freshwater and marine habitats", Microb Ecol, 21, pp.99~118 (1991)