본문 바로가기
KERD
논문KCI 등재

병원성 미생물에 대한 Lactobacillus bulgaricus SP5의 항균활성

Antibacterial Activity by Lactobacillus bulgaricus SP5 against Pathogenic Bacteria

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

초록

This study was carried out to get basic resources for the industrial use of Lactobacillus bulgaricus SP5. The antibacterial activity of the supernatant obtained from Lactobacillus bulgaricus SP5 was tested against the pathogenic bacteria such as Escherichia coli KCCM 11234, Salmonella enteritidis KCCM 3313, Salmonella enteritidis KCCM 12021, and Salmonella typhimurium KCCM 40253. The supernatant of L. bulgaricus SP5 showed antibacterial activity against tested pathogenic bacteria. The antibacterial activity was examined after adjusting pH and heat treatment of supernatant. Heat treatment of supernatant had antibacterial activity against pathogenic bacteria at all temperature. However, pH changes showed no antibacterial activity. Antibacterial activity of the supernatant was confirmed to be due to organic acids (lactic, acetic, phosphoric, succinic, pyroglutamic, citric, malic, and formic acid).

Abstract

This study was carried out to get basic resources for the industrial use of Lactobacillus bulgaricus SP5. The antibacterial activity of the supernatant obtained from Lactobacillus bulgaricus SP5 was tested against the pathogenic bacteria such as Escherichia coli KCCM 11234, Salmonella enteritidis KCCM 3313, Salmonella enteritidis KCCM 12021, and Salmonella typhimurium KCCM 40253. The supernatant of L. bulgaricus SP5 showed antibacterial activity against tested pathogenic bacteria. The antibacterial activity was examined after adjusting pH and heat treatment of supernatant. Heat treatment of supernatant had antibacterial activity against pathogenic bacteria at all temperature. However, pH changes showed no antibacterial activity. Antibacterial activity of the supernatant was confirmed to be due to organic acids (lactic, acetic, phosphoric, succinic, pyroglutamic, citric, malic, and formic acid).

참고문헌 (20)

  1. Anderson, M. E., "Reducing microbial populations on beef tissues: Concentration and temperature of an acid mixture", J. Food Sci, 55, pp.903~905 (1990)
  2. Bhunia, A. K., "Direct detection and antimicrobial of Pediococcus acidilatici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis", J. Ind. Microbiol. Biotechnol, 2, pp.319~322 (1987)
  3. Daba, H. S., "Detection and activity of a bacteriocin produced by Leuconostoc mesenteroides", Appl. Environ. Microbiol, 57, pp.3450~3455 (1991)
  4. Deegan, L. H., "Bactriocins: Biological tools for bio-preservation and shelf-life extension", Int. Dairy J, 16, pp.1058~1071 (2006)
  5. Drider, D., "The continuing story of class IIa bacteriocins", Microbiol. Mol. Biol. Rev, 70, pp.564~582 (2006)
  6. Fukushima Y., "Effect of a probiotic in healthy children", Int. J. Food Microbiol, 42, pp.39~44 (1998)
  7. Gill, C. O., "Effect of lactic acid concentration on growth on meat of gram-negative psychrotrophs from a meatworks", App. Environ. Microbiol, 43, pp.284~288 (1982)
  8. Homma, N., "Bifidobacteria as a resistance factor in human beings", Bifidobact. Microfl, 7, pp.35~43 (1998)
  9. 장정순, "새롭게 출현한 Arcobacter butzleri의 유기산과 trisodium phosphate 처리에 의한 생육저해효과", 한국식품과학회지, 35(6), pp.1169~1173 (2003), 한국식품과학회
  10. Jung, H. M., "Effects of organic acids on the storability of chilled beef", Korean J. Food Sci. Technol, 23, pp.379~387 (1991)
  11. 김수영, "김치에서 항균활성 유산균의 분리 및 동정", 한국식품과학회지, 43(4), pp.446~452 (2011), 한국식품과학회
  12. Laemmli, U., "Cleavage of structural proteins during the assembly of the head of bacteriophage T4", Nature (London), 227, pp.680~686 (1970)
  13. Lee, J. L., "Utilization of fermented milk and it’s health promotion", Korean Dairy Techno, 17, pp.58~71 (1999)
  14. Nakae, T., "Utilization of lactic acid bacteria in animal industry: recent outlook", Jpn. J. Zootech. Sci, 57, pp.279~287 (1986)
  15. Peres, C. M., "Review on fermented plant materials as carriers and sources of potentially probiotic lactic acid bacteria- with an emphasis on table olives", Trends in Food Sci. Technol, 26 (2012)
  16. Pfeiler, E. A., "The genomics of lactic acid bacteria", Trends in Microbiology, 15, pp.546~553 (2007)
  17. Ray, B., "Impact of bacterial injury and repair in food microbiology: Its past, present and future", J. Food Prot, 49, pp.651~670 (1986)
  18. Ray, B., "Food biopreservatives of microbial origin", pp.207~ (1992), CRC Press
  19. SAS, "SAS User`s Guide", Statistical Analysis System Institute, ~ (2001)
  20. Yoshitaka, H., "Daily intake of heat-killed Lactobacillus plantarum L-137 augments acquired immunity in healthy adults", J. Nutr, 136, pp.3069~3073 (2006)

관련 논문AI추천

이 간행물의 다른 논문 (18건)