김치에서 분리한 Lactobacillus plantarum LHB55의 항균성과 요구르트 제조 적합성 연구
A Study on the Yogurt Manufacture Suitability and Antimicrobial Activity of Lactobacillus plantarum LHB55 Isolated from Kimchi
2
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
이 연구는 김치에서 분리한 항균활성이 우수한 요구르트 제조용 유산균 스타터를 개발하기 위함이다. 분리한 103개의 산 생성 균주를 PCR로 screening하여 72개의 유산균을 분리하였다. 분리균의 배양액을 paper disk method를 사용하여 병원성 미생물(E. coli, S. enteritidis, S. aureus)에 대한 항균활성을 측정하였고, 활성이 강한 균주를 선별하여 API 50CHL과 16S rRNA sequencing 방법으로 균을 동정하였다. 균은 L. plantarum으로 확인되어 L. plantarum LHB55로 명명하였다. E. coli에 대해 높은 항균효과를 나타내는 L. plantarum LHB55를 사용하여 제조한 요구르트의 미생물학적, 이화학적 특성과 관능검사 결과 대조구와의 유의적인 차이는 거의 없었다. 그 결과 김치에서 분리한 L. plantarum LHB55의 항균활성이 우수한 요구르트 제조 스타터 균주로서 사용 가능함을 확인하였다. 항균성 등 인간에게 유리하게 작용하는 스타터 균주와 발효유제품 개발은 계속적인 연구가 필요할 것으로 생각된다.
Abstract
The aim of this study was to develop a new starter for fermented milk. The approach started with 103 acid-producing isolates from Kimchi, a type of spiced, fermented cabbage and then PCR screening was used to identify 72 Lactobacillus strains. The ability to inhibit the growth of food-borne human pathogens(Escherichia coli, Salmonella enteritidis, Staphylococcus aureus) of these strains were measured, using the paper disk method. Among them, one bacterium(LHB55) that showed a strong antibacterial activity against food-borne human pathogens was identified and further characterized, using 16S rDNA sequencing and API 50CHL system. Because this isolate was identified as L. plantarum, it was named as L. plantarum LHB55. The yogurt produced using commercial LAB with L. plantarum LHB55 did not display properties that are microbially or physico-chemically different from the control group, which suggests that L. plantarum LHB55 can be used as a useful starter for yogurt containing high antibacterial activity. We think that identifying effective starter strains enabling further development of fermented milk that can deliver better health benefits such as antimicrobial properties is of high significance, and thus our effort in this type of approach will continue.
참고문헌 (27)
- DeDios, Pozo-Olano, J., "Effect of a lactobacilli preparation on traveler’s diarrhea", Gastroenterol, 74, pp.829~839 (1978)
- Deurenberg, R. H., "The molecular evolution of methicillin-resistant Staphylococcus aureus", Clin. Microbiol. Infect., 13, pp.222~235 (2007)
- Francis, A. W., "Staphylococcus aureus(including toxic shock syndrom). in: Mandell Douglas and Bennetts Principles Bennett’s Principles & Practice of Infections Disease. 4th eds.", pp.1754~1755 (1995), Churchill Livingstone
- Han, S. J., "Multiple intestinal ulcerations and perforations secondary to methicillin-resistant Staphylococcus aureus enteritis in infants", J. Pediatr. Surg., 34, pp.381~386 (1999)
- 전상록, "국내 액상 발효유용 유산균 스타터 미생물의 동정 및 생리적 특성", 한국축산식품학회지, 27(4), pp.509~516 (2007), 한국축산식품학회
- Jeong, H. J., "Nosocomial infection surveillance in the care unit", Kor. J. Infect. Dis., 27, pp.105~117 (1995)
- Kim, S. I., "Isolation and identification of antimicrobial agent producing microorganisms and sensitive strain from soil", J. Kor. Soc. Food Sci. Nutr., 28, pp.526~533 (1999)
- Klaenhammer, T. R., "Bacteriocins of lactic acid bacteria", Bichimie, 70, pp.337~349 (1988)
- Ko, Y. D., "Development of control system for Kimchi fermentation and storage using refrigerator", Kor. J. Food Sci. Technol., 26, pp.199~203 (1994)
- Lee, I. S., "Changes in some characteristics of brined chinese cabbage of fall cultivars during storage", Kor. J. Food Sci. Technol., 26, pp.239~245 (1994)
- 이승규, "마늘즙 첨가 요구르트의 품질특성 및 항생제 저항성 황색포도상구균 KCCM 40510 억제활성", 한국축산식품학회지, 29(4), pp.500~505 (2009), 한국축산식품학회
- Sung Mee Lim, "Bactericidal Effect of Bacteriocin of Lactobacillus plantarum K11Isolated from Dongchimi on Escherichia coli O157", 한국식품위생안전성학회지, 22(3), pp.151~158 (2007), 한국식품위생안전성학회
- 임성미, "Screening and Characterization of Probiotic Lactic Acid Bacteria Isolated from Korean Fermented Foods", Journal of Microbiology and Biotechnology, 19(2), pp.178~186 (2009), 한국미생물·생명공학회
- Mheen, T. I., "Effect of temperature and salt concentration on Kimchi fermentation", Kor. J. Food Sci. Technol., 16, pp.443~450 (1984)
- Muller, D. M., "Characterization and purification of a new bacteriocin with a broad inhibitory spectrum produced by Lactobacillus plantarum lp31 strain isolated from dry-fermented sausage", J. Appl. Microbiol., 106, pp.2031~2040 (2009)
- Nguyen, T. D., "Characterization of Lactobacillus plantarum PH04, a potential probiotic bacterium with cholesterol-lowering effects. 2007", Int. J. Food Microbiol., 113, pp.358~361 (2007)
- Park, S. G., "Biological characteristics of Staphylococcus aureus isolated from food-borne patients in Seoul", J. Food Hyg. Saf., 16, pp.159~167 (2001)
- Park, W. S., "Kimchi preparation with brined chinese cabbage and seasoning mixture stored separately", Kor. J. Food Sci. Technol., 26, pp.231~238 (1994)
- Rhee, Y. H., "Characteristics of beta- galactosidase activity in Lactobacillus plantarum from Kimchi", Agr. Chem. Biotechnol., 39, pp.60~66 (1996)
- Rhee, Y. H., "Physico-chemical characteristics and ß-galactosidase activity of Lactobacillus plantarum from Kimchi", Agr. Chem. Biotechnol., 39, pp.54~59 (1996)
- Sanders, M. E., "Bringing a probiotic- containing functional food to the market: microbiological, product, regulatory and labeling issues", Antonie Van Leeu- wenhoek, 76, pp.293~315 (1999)
- So, M. H., "Cultural characteristics psychrotrophic lactic acid bacteria isolated from Kimchi", Kor. J. Food Sci. Technol., 27, pp.506~515 (1995)
- Straume, D., "DNA binding kinetics of two response regulators, PlnC and PlnD, from the bacteriocin regulon of Lactobacillus plantarum C11", BMC. Biochem., 10, pp.17~27 (2009)
- Taylor, J., "The treatment of bovine streptococcal and staphylococcal mastitis with nisin", Vet. Rec., 61, pp.197~198 (1949)
- Tsai, T. y., "Atherosclerosis-preventing activity of lactic acid bacteria- fermneted milk-soymilk supplemented with Momordica charantia", J. Agric. Food Chem., 57, pp.2065~2071 (2009)
- Wang, Y., "Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet", Appl. Microbiol. Biotechnol., 84, pp.341~347 (2009)
- Yoon, J. H., "Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences", Int. J. Syst. Bacteriol., 48, pp.187~194 (1996)