친환경 흑염소 사양을 위한 최적 조사료 초종 및 TMR혼합비 비율: In vitro 반추위 발효 연구
Optimum Forage Sources and Its Ratio in TMR for Environmently-friendly Goat Feeding:In vitro Rumen Fermentation Study
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This study was conducted to investigate the effects of TMR on in vitro rumen fermentation and methane production of goat with different forage sources. The experiment was arranged 4×2 factorial design. The different forage sources were rice straw (RS), Italian rye grass (IR), timothy (TI) and alfalfa (AL), respectively. There were two different forage : concentrate ratios such as 20:80 (20) and 50:50 (50), respectively. Therefore, totally 8 treatments were used: 1) RS20, 2) RS50, 3) IR20, 4) IR50, 5) TI20, 6) TI50, 7) AL20, and 8) AL50, respectively. The rumen fluid of goat was collected from the slaughterhouse. For fermentation parameters, ruminal pH, total gas, methane, hydrogen, ammonia nitrogen, and volatile fatty acid were determined. The pH values were within an optimal range across all treatments. Total gas productions at TI20 and AL50 were significantly greater than others (p<0.05). Methane production was significantly lower in TI and AL compared with other treatments (p<0.05). The relatively high dietary NDF content in treatments showed significantly lower methane production (p<0.05). Significant alterations treatments were detected at ammonia nitrogen concentration according to the ratio of forage : concentrate (p<0.05). AL treatment showed greater total volatile fatty acid production compared with other treatments (p<0.05). Therefore, the present study suggests that both Timothy and Alfalfa could be recommendable forage sources for goat based on results with volatile fatty acid as an energy source and methane as an index for energy loss and environmental issues. Also, the 50:50 (forage:concentrate) ratio would prefer to 20:80.
Abstract
This study was conducted to investigate the effects of TMR on in vitro rumen fermentation and methane production of goat with different forage sources. The experiment was arranged 4×2 factorial design. The different forage sources were rice straw (RS), Italian rye grass (IR), timothy (TI) and alfalfa (AL), respectively. There were two different forage : concentrate ratios such as 20:80 (20) and 50:50 (50), respectively. Therefore, totally 8 treatments were used: 1) RS20, 2) RS50, 3) IR20, 4) IR50, 5) TI20, 6) TI50, 7) AL20, and 8) AL50, respectively. The rumen fluid of goat was collected from the slaughterhouse. For fermentation parameters, ruminal pH, total gas, methane, hydrogen, ammonia nitrogen, and volatile fatty acid were determined. The pH values were within an optimal range across all treatments. Total gas productions at TI20 and AL50 were significantly greater than others (p<0.05). Methane production was significantly lower in TI and AL compared with other treatments (p<0.05). The relatively high dietary NDF content in treatments showed significantly lower methane production (p<0.05). Significant alterations treatments were detected at ammonia nitrogen concentration according to the ratio of forage : concentrate (p<0.05). AL treatment showed greater total volatile fatty acid production compared with other treatments (p<0.05). Therefore, the present study suggests that both Timothy and Alfalfa could be recommendable forage sources for goat based on results with volatile fatty acid as an energy source and methane as an index for energy loss and environmental issues. Also, the 50:50 (forage:concentrate) ratio would prefer to 20:80.
참고문헌 (22)
- Chaney, A. L, "Modified reagents for determination of urea and ammonia", Clin. Chem, 8, pp.130~132 (1962)
- Erwin, E. S, "Volatile fatty acid analyses of blood and rumen fluid by gas chromatography", J. Dairy. Sci, 44, pp.1768~1771 (1961)
- Hiltner, P, "Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria", Appl. Environ. Microbiol, 46, pp.642~648 (1983)
- 이성훈, "사료 내 조농비율에 따른 생균제의 첨가가 흑염소의 사료섭취량, 영양소 소화율 및 질소축적에 미치는 영향", 한국유기농업학회지, 15(2), pp.195~205 (2007), 한국유기농업학회
- 지병주, "소에 의한 다양한 사일리지의 이용율 및 TDN 평가", 한국초지조사료학회지, 30(2), pp.169~178 (2010), 한국초지조사료학회
- 정기웅, "사료급여체계가 재래종 및 교잡종 흑염소의 영양소 이용율, 질소축적 및 혈액성상에 미치는 영향", 한국초지조사료학회지, 28(4), pp.341~350 (2008), 한국초지조사료학회
- 정기웅, "동계사료작물 사일리지를 첨가한 TMR 급여가 흑염소의 사료섭취량, 영양소 소화율 및 혈액성상에 미치는 영향", 한국초지조사료학회지, 29(4), pp.389~398 (2009), 한국초지조사료학회
- 김지융, "말에서 국내산 이탈리안 라이그라스 사일리지의 수입산 티모시 건초 대체 효과", 한국초지조사료학회지, 40(1), pp.44~49 (2020), 한국초지조사료학회[DOI]
- Kim, K. J, "Effects of roughage to concentrate ratio and roughage source on ruminal characteristics and methane production in Hanwoo", Kor. J. Anim. Nutr. Feed, 23(5), pp.381~390 (1999)
- 김광국, "총체 보리사일리지를 첨가한 유기 TMR 급여가 흑염소의 사료섭취량, 영양소 소화율 및 혈액성상에 미치는 영향", 한국유기농업학회지, 15(4), pp.413~424 (2007), 한국유기농업학회
- 김상욱, "옥수수 첨가 TMR 사료 급여가 거세흑염소의 발육과 육질에 미치는 영향", 한국초지조사료학회지, 36(4), pp.350~356 (2016), 한국초지조사료학회
- 김상우, "볏짚 위주 사양 시 육성 흑염소의 농후사료 급여 수준이 발육, 사료이용성 및 경제성에 미치는 영향", 한국초지조사료학회지, 33(4), pp.298~303 (2013), 한국초지조사료학회
- Knapp, J. R, "Invited review: Enteric methane in dairy cattle production: Quantifying the opportunities and impact of reducing emissions", J. Dairy. Sci, 97, pp.3231~3261 (2014)
- Lee, S, "Effects of chemical treatments and ensiling on the chemical composition and degradation rate in the rumen", J. Kor. Grassl. Forage Sci, 20(3), pp.177~184 (2000)
- 이덕윤, "유형별 완전혼합사료 급여가 반추위내의 발효성상 및영양소 소화율에 미치는 영향", 한국축산학회지, 45(5), pp.805~812 (2003), 한국축산학회
- Moe, P. W, "Methane production in dairy cows", J. Dairy. Sci, 62, pp.1583~1586 (1979)
- Nocek, J. E, "Effect of mixed ration nutrient density on milk of cows transferred from high production group", J. Dairy Sci, 68, pp.133~ (1985)
- 오성진, "반추가축전용 기후변화대응 비식량자원 거대억새의 생육부위 및 시기에 따른 체내 이용가치 비교 연구", 한국유기농업학회지, 21(3), pp.437~450 (2013), 한국유기농업학회
- 류채화, "환경친화적 섬유질 배합사료의 발효와 반추위 발효특성 변화", 한국유기농업학회지, 25(2), pp.461~473 (2017), 한국유기농업학회
- Tilley, J. M. A, "A two-stage technique for the in vitro digestion of forage crops", J. Br. Grassl. Soc, 18, pp.104~111 (1963)
- Troelsen, J. E, "Ruminant digestion in vitro as affected by inoculum donor, collection day, and fermentation time", Canadian J. Anim. Sci, 46, pp.149~156 (1966)
- Woo, Y. W, "Effects of extruded linseed supplementation on methane production in Holstein steers. J. Kor. Grassl", Forage Sci, 37(4), pp.315~321 (2017)