은행 외종피로부터 살선충 물질의 순수 분리와 활성
Purification Nematicidal Substance and NematicidalActivity from Ginkgo biloba L. Outer Seedcoat
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Plant parasitic nematodes are causing significant damage in crop production. There is a need to develop eco-friendly nematicide that reduces the damage of nematode and has little effect on the environment and human. In this study, we have isolated a substance having nematicidal activity from Ginkgo biloba L. outer seedcoat. Studies of G. biloba L. outer seedcoat are insufficient compared to the seed and leaves due to their odor and toxicity. The dried G. biloba L. outer seedcoat was extracted with dichloromethane:methanol (1:1) and fractionated into hexane, ethyl acetate and H2O. Four steps TLC were performed from EtOAc fraction to purely isolate GB4-3 with nematicidal activity. To compare nematicidal activity, G. biloba L. seedcoat methanol extract and purified GB4-3 were investigated in terms of treatment concentration and time. As a result, the nematicidal activity increased with concentration and time. In the place treated with 20 µg/mL of crude G. biloba L. seedcoat MeOH extract, strong activity appeared after 12 hours, and 46% nematicidal activity shown after 18 hours. About 69% of nematicidal activity was confirmed in the place where GB4-3 purified from outer seedcoat was treated with 20 µg/mL, and the possibility of development as nematicide was very high. This study could be used as a basic data for the development of a nematode preparation from G. biloba L. outer seedcoat.
Abstract
Plant parasitic nematodes are causing significant damage in crop production. There is a need to develop eco-friendly nematicide that reduces the damage of nematode and has little effect on the environment and human. In this study, we have isolated a substance having nematicidal activity from Ginkgo biloba L. outer seedcoat. Studies of G. biloba L. outer seedcoat are insufficient compared to the seed and leaves due to their odor and toxicity. The dried G. biloba L. outer seedcoat was extracted with dichloromethane:methanol (1:1) and fractionated into hexane, ethyl acetate and H2O. Four steps TLC were performed from EtOAc fraction to purely isolate GB4-3 with nematicidal activity. To compare nematicidal activity, G. biloba L. seedcoat methanol extract and purified GB4-3 were investigated in terms of treatment concentration and time. As a result, the nematicidal activity increased with concentration and time. In the place treated with 20 µg/mL of crude G. biloba L. seedcoat MeOH extract, strong activity appeared after 12 hours, and 46% nematicidal activity shown after 18 hours. About 69% of nematicidal activity was confirmed in the place where GB4-3 purified from outer seedcoat was treated with 20 µg/mL, and the possibility of development as nematicide was very high. This study could be used as a basic data for the development of a nematode preparation from G. biloba L. outer seedcoat.
참고문헌 (22)
- Abo-Elyousr, K. A. M., "Biological Control of the Tomato Wilt Caused by Clavibacter michiganensis subsp. michiganensis Using Formulated Plant Growth-promoting Bacteria", Egyp. J. Biol. Pest Control, 29, pp.54~61 (2019)
- Atkins, S. D., "Approaches for Monitoring the Release of Pochonia chlamydosporia var. catenulata, a Biocontrol Agent of Root-knot Nematodes", Mycol. Res., 107(2), pp.206~212 (2003)
- Choi J. G., "Antibactrial Activity of Hydroxyalkenyl Salicylic Acids from Sarcotesta of Ginkgo biloba Against Vancomycin-Resistant Enterococcus", Fitoterapia, 80, pp.18~20 (2009)
- Choudhary, G. P, "Anthelmintic Activity of Leaves of Coleus aromaticus Benth", IJAPBC, 2(4), pp.609~610 (2013)
- 한상빈, "은행잎과 외종피 유래 바이오농약 연구동향", 농약과학회지, 18(3), pp.210~219 (2014), 한국농약과학회[DOI]
- Katarzyna, D., "Synthesis and Anthelmintic Activity of New Thiosemicarbazide Derivatives — A Preliminary Study", Molecules, 25(12), pp.2770~2777 (2020)
- Kim, D. G., "Effects of Incorporation Method of Nematicides on Reproduction of Meloidogyne arenaria", Korean J. Appl. Entomol., 40(1), pp.89~95 (2001)
- 김동근, "선충 억제 활성 식물 추출물의 선발", 식물병 연구, 20(1), pp.37~49 (2014), 한국식물병리학회[DOI]
- 이인화, "포도상구균에 대한 에탄올 농도별 은행잎 추출물의 항균효과에 관한 연구", KSBB Journal, 21(4), pp.312~316 (2006), 한국생물공학회
- 이인화, "점박이응애(Tetranichus urticae), 목화진딧물(Aphis gossypii)과복숭아혹진딧물(Myzus persicae)에 대한 은행잎 추출물의살충 및 기피효과", Applied Biological Chemistry, 48(2), pp.150~154 (2005), 한국응용생명화학회
- Leistner E., "Ginkgo biloba and Ginkgotoxin", J. Nat. Prod., 73(1), pp.86~92 (2010)
- 김성문, "고구마뿌리혹선충(Meloidogyne incognita)에 대한 국내 자생식물의 살선충 활성", 농약과학회지, 8(4), pp.353~357 (2004), 한국농약과학회
- McGaw, L. J., "In vitro Anthelmintic, Antibacterial and Cytotoxic Effects of Extracts from Plants Used in South African Ethnoveterinary Medicine", Veterinary J, 173(2), pp.366~372 (2007)
- Pan, W., "Acaricidal Activity Against Panonychus citri of a Ginkgolic acid from the External Seed Coat of Ginkgo biloba", Pest Manag. Sci., 62, pp.283~287 (2006)
- 박샛별, "은행 잎, 종실 및 외종피 추출물의 항균활성", 한국식품영양과학회지, 40(1), pp.7~13 (2011), 한국식품영양과학회
- Siddiqui Z. A., "Role of Bacteria in the Management of Plant Parasitic Nematodes : A Review", Bioresource Technol, 69(2), pp.167~179 (1999)
- 소상섭, "Insecticidal activities of Ginkgo biloba seed coat extracts against Spodoptera exigua (Lepidoptera: Noctuidae)", Entomological Research, 42(3), pp.158~162 (2012), 한국곤충학회
- Søholm, B, "Clinical Improvement of Memory and Other Cognitive Functions by Ginkgo biloba: Review of Relevant Literature", Adv. Ther., 15(1), pp.54~65 (1998)
- Spiegel, Y., "Evaluation of a Newly Isolated Bacterium, Pseudomonas chitinolytica sp. nov., for Controlling the Root‐knot Nematode Meloidogyne javanica", Biocont. Sci. Technol., 1(2), pp.115~125 (1991)
- Yang, E. Y., "Insecticidal Activities of Bilobalide from Ginkgo biloba Leaves and its Derivatives", Korean J. Pestic. Sci., 5(1), pp.24~29 (2001)
- Yao, P., "Ginkgo biloba Extract Prevents Ethanol Induced Dyslipidemia", Am. J. Chin. Med., 35(4), pp.643~652 (2007)
- 임춘근, "Pasteuria penetrans의 처리방법에 따른 땅콩뿌리혹선충(Meloidogyne arenaria) 방제효과", 농약과학회지, 9(4), pp.437~441 (2005), 한국농약과학회