본문 바로가기
KERD
논문KCI 등재

Dermal and Eye Irritation Studies on Bactonematicide, Photorhabdus temperata Isolated from Heterorhabditis megidis (Nematoda: Heterorhabditidae) in Rabbit

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

The acute dermal and eye irritation tests of Photorhabdus temperata, a symbiotic bacterium of Heterorhabditis megidis Gwangju strain, were carried out in New Zealand white rabbit (Oryctolagus cuniculus), following the guidelines of OECD and Rural Development Administration (RDA) of Korea. In both tests, neither dermal nor eye responses were found from all the P. temperata treated rabbits and the results were classified as non-irritating. That is, erythema, eschar, edema, and any other dermal critical signs were not observed from all the experimental rabbits in the dermal irritation test. In the acute eye irritation test, no clinical signs of cornea, iritis, conjunctiva (redness, edema, lacrima, and chemosis) were observed from all the experimental rabbits. Individual ocular irritation, mean ocular irritation, and acute ocular irritation were calculated as 0.0. The results of dermal and eye irritation studies on P. temperata indicated that this bacterium could be a safe and effective alternative bionematicide against the most serious plant-parasitic root-knot nematodes in the genus Meloidogyne.

Abstract

The acute dermal and eye irritation tests of Photorhabdus temperata, a symbiotic bacterium of Heterorhabditis megidis Gwangju strain, were carried out in New Zealand white rabbit (Oryctolagus cuniculus), following the guidelines of OECD and Rural Development Administration (RDA) of Korea. In both tests, neither dermal nor eye responses were found from all the P. temperata treated rabbits and the results were classified as non-irritating. That is, erythema, eschar, edema, and any other dermal critical signs were not observed from all the experimental rabbits in the dermal irritation test. In the acute eye irritation test, no clinical signs of cornea, iritis, conjunctiva (redness, edema, lacrima, and chemosis) were observed from all the experimental rabbits. Individual ocular irritation, mean ocular irritation, and acute ocular irritation were calculated as 0.0. The results of dermal and eye irritation studies on P. temperata indicated that this bacterium could be a safe and effective alternative bionematicide against the most serious plant-parasitic root-knot nematodes in the genus Meloidogyne.

참고문헌 (66)

  1. Aatif, H. M., "Virulence of Xenorhabdus and Photorhabdus bacteria and their toxins against juvenile’s immobilization of Meloidogyne incognita", Pak. J. Phytopathol, 24, pp.170~174 (2012)
  2. Abd-Elgawad, M. M. M., "Biological control agents of plant-parasitic nematodes", Egyptian Journal of Biological Pest Control, 26, pp.423~429 (2016)
  3. Akhurst, R., "Entomopathogenic nematology", pp.311~332 (2002), CABI Publishing
  4. Akurst, R. J., "Morphological and Functional Dimorphism in Xenorhabdus spp., Bacteria Symbiotically Associated with the Insect Pathogenic Nematodes Neoaplectana and Heterorhabditis", Journal of General Microbiology, 121, pp.303~309 (1980)
  5. Artega, M. E., "Dermal toxicity, eye and dermal irritation and skin sensitization evaluation of new formulation of Bacillus thuringiensis var israelensis SH-14", Regulatory Toxicology and Pharmacology, 68, pp.147~151 (2014)
  6. Bedding, R. A., "A simple technique for the detection of insect parasitic rhabditid nematodes in soil", Nematologica, 21, pp.109~110 (1975)
  7. Belien T., "Entomopathogenic nematodes as biocontrol agents of insect pests in orchards", CAB Review, 15, pp.11~ (2018)[DOI]
  8. Boemare, N, "Entomopathogenic nematology", pp.35~56 (2002), CABI Publishing
  9. Boemare, N., "The entomopathogenic nematode-bacterium complex : biology, life cycle and vertebrate safety", Biocontrol Science and Technology, 28, pp.333~346 (1996)
  10. Burnell, A. M., "Heterorhabditis, Steinernema and their bacterial symbionts-lethal pathogens of insects", Nematology, 2, pp.31~42 (2000)
  11. Chacon-Orozco, J. G., "Antifungal activity of Xenorhabdus spp., and Photorhabdus spp. against soybean pathogenic Sclerotinia sclerotiorum", Scientific Reports, 10, pp.20649~ (2020)
  12. Chandra, S. A., "Dermal toxicity studies : factors impacting study interpretation and outcome", Toxicologic Pathology, 43, pp.474~481 (2015)
  13. Chapman, C., "Identification and characterization of Photorhabdus temperata mutants altered in hemolysis and virulence", Canadian Journal of Microbiology, 62, pp.657~667 (2016)
  14. Chen, G., "Chitinase activity of Xenorhabdus and Photorhabdus species, bacterial associates of entomopathogenic nematodes", J Invertebr Pathol, 68, pp.101~108 (1996)
  15. 조명래, "감자 주산지 식물기생선충 분포와 뿌리혹선충의 PCR 동정", 한국응용곤충학회지, 45(1), pp.79~85 (2006), 한국응용곤충학회
  16. Choi, Y. E., "A study on the root-knot nematodes (Meloidogyne spp.)affecting economic crops in Korea", Korea J. Pl. Prot, 17, pp.89~98 (1978)
  17. Choo, H. Y., "A study on the plant parasitic nematodes (Tylenchida)in Jeju province", Kor. J. Pl. Prot, 18, pp.169~175 (1979)
  18. Choo, H. Y., "Isolation of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Korea", Japan. J. Nematol, 25, pp.44~51 (1995)
  19. Choo, H. Y., "Entomopathogenic nematodes (Steinernema spp., and Heterorhabditis bacteriophora)and a fungus Beauveria brongniartii for biological control of the white grubs, Ectinohoplia rufipes and Exomala orientalis, in Korean golf courses", BioControl, 47, pp.177~192 (2002)
  20. Choo, H. Y., "Microbial insecticides, principles and applications", pp.151~192 (2011), Nova Science Publishers, Inc
  21. Choo, H. Y., "Influence of Meloidogyne incognita infection on the development of cucumber wilt by Fusarium oxysporum f. sp. cucumerium", The Plant Pathology Journal, 6, pp.412~416 (1990)
  22. Choo, H. Y., "Relationship of root-knot nematode, Meloidogyne incognita to pathogenesis of Rhizoctonia solani on cucumber, pepper, and tomato", Korean Journal of Plant Pathology, 6, pp.409~411 (1990)
  23. Dreyer, J., "Bacteria of the genus Xenorhabdus, a novel source of bioactive compounds", Front Microbial, 9, pp.14~ (2018)
  24. Ehlers, R. U., "Mass production of entomopathogenic nematodes for plant protection", Appl Microbiol Biotechnol, 56, pp.623~633 (2001)
  25. Ehlers, R. U., "Entomopathogenic nematodes in the European biocontrol market", Communications in Agricultural and Applied Biological Sciences, 68, pp.3~16 (2003)
  26. Escobar, C., "Advances in botanical research", pp.1~32 (2015), Academic Press
  27. Gupta, S., "Biopesticides : an ecofriendly approach for pest control", Journal of Biopesticides, 3, pp.186~188 (2010)
  28. Han, R., "Trans-specific nematicidal activity of Photorhabdus luminescens", Nematology, 1, pp.687~693 (1999)
  29. Hazir, S., "Entomopathogenic nematodes(Steinernematidae and Heterorhabditidae)for biological control of soil pests", Turk J Biol, 27, pp.181~202 (2003)
  30. Hinchliffe, S. A. J., "Insecticidal toxins from the Photorhabdus and Xenorhabdus bacteria", The Open Toxinology Journal, 3, pp.83~100 (2010)
  31. Hominick, W. M, "Entomopathogenic nematology", pp.115~143 (2002), CABI Publishing
  32. Hu, K., "Mortality of plant-parasitic nematodes caused by a bacterial (Xenorhabdus spp., and Photorhabdus luminescens) culture media", Journal of Nematology, 27, pp.502~503 (1995)
  33. Hu, K., "Nematicidal metabolites produced by Photorhabdus luminescens (Enterobacteriaceae), bacterial symbiont of entomopathogenic nematodes", Nematology, 1, pp.457~469 (1999)
  34. Jung, Y. H., "Taxonomic studies on insect parasitic nematodes from Korea", Ph.D, pp.2016
  35. Kaya, H. K., "Manual of techniques in insect pathology", pp.281~324 (1997), Academic Press
  36. Kaya, H. K., "Entomopathogenic nematodes", Annu. Rev. Entomol, 38, pp.181~206 (1993)
  37. Kaya, H. K., "Status of entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions of the world", Biological Control, 38, pp.134~155 (2006)
  38. 김형환, "작은뿌리파리에 대한 한국산 곤충 병원성 선충의 방제 효과와 상토에서의 지속성", Horticulture, Environment, and Biotechnology, 44(3), pp.393~401 (2003), 한국원예학회
  39. Kumar, S., "Use of pesticides in agriculture and livestock animals and its impact on environment of India", Asian Journal of Environmental Science, 8, pp.51~57 (2013)
  40. Kumar, V., "A review on efficacy of biopesticides to control the agricultural insect’s pest", International Journal of Agricultural Science Research, 4, pp.168~179 (2015)
  41. Lacey, L. A., "Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production", J. Nematol, 44, pp.218~225 (2012)
  42. 이보승, "Burkholderia Pyrrocinia CAB08106-4의 국소자극성시험 및 피부감작성시험", 농약과학회지, 23(2), pp.126~133 (2019), 한국농약과학회[DOI]
  43. 이제봉, "국내 공시 유기농업자재의 독성과 안전성평가", 농약과학회지, 22(3), pp.184~191 (2018), 한국농약과학회[DOI]
  44. Machado, R. A. R., "Whole-genome-based revisit of Photorhabdus phylogeny: proposal for the elevation of most Photorhabdus subspecies to the species level and description of one novel species Photorhabdus bodei sp. nov., and one novel subspecies Photorhabdus laumondii subsp. clarkei subsp. nov.", International Journal of Systematic and Evolutionary Microbiology, 68, pp.2664~2681 (2018)
  45. Moens, M., "Root-knot nematodes", pp.1~17 (2009), CAB International
  46. Nour El-Deen, A. H., "Nematicidal activity of entomopathogenic bacteria against root-knot nematodes, Meloidogyne incognita in-vitro", International Journal of Advanced Research, 2, pp.708~713 (2014)
  47. Obendorf, D. L., "Non-susceptibility of mammals to the entomopathogeniuc bacterium X. nematophilus", Environmental Entomology, 12, pp.368~370 (1983)
  48. OECD, "Guideline for the testing of chemicals 405. Acute eye irritation", corrosion, 2012
  49. OECD, "OECD Guideline for the testing of chemicals 404. Acute dermal irritation", corrosion, 2015
  50. OECD, "Guideline for the testing of chemicals 405. Acute eye irritation", corrosion, 2017
  51. 박홍현, "천연제초활성 소재 Herbicidin의 설치류와 토끼에 대한 독성평가", 농약과학회지, 24(4), pp.334~342 (2020), 한국농약과학회[DOI]
  52. Poinar, G. O., "Inoculation of entomogenous nematodes, Neoaplectana and Heterorhabditis, and their associated bacteria, Xenorhabdus spp., into chicks and mice", Environ. Entomol, 11, pp.137~138 (1982)
  53. Ralmi, N. H. A. A., "Occurrence and control of root knot nematode in crops : a review", Australian Journal of Crop Science, 10, pp.1649~1654 (2016)
  54. RDA, "Criteria for registration of pesticides and active substances. Legislation and notification directive for pesticide regulation", RDA, ~ (2012)
  55. RDA, "Criteria for registration of pesticides and active substances. Legislation and notification directive for pesticide regulation", RDA, ~ (2015)
  56. RDA, "Criteria for registration of pesticides and active substances. Legislation and notification directive for pesticide regulation", RDA, ~ (2017)
  57. Sajnaga, E., "Evolution and taxonomy of nematode-associated entomopathogenic bacteria of the genera Xenorhabdus and Photorhabdus : an overview", Symbiosis, 80, pp.1~13 (2020)
  58. Salas, A., "Nematicidal effect of an Argentine strain of Photorhabdus laumondi laumondi(Enterobacteriaceae)on the free-living nematode Panagrellus redivivus(Rhabditidae : Panagrolaimidae)", Revista Argentina de Microbiologia, 53, pp.84~85 (2020)
  59. Samaliev, H. Y., "The nematicidal effects of the bacteria Pseudomonas oryzihabitans and Xenorhabdus nematophilus on the root-knot nematode Meloidogyne javanica", Nematology, 2, pp.507~514 (2000)
  60. Sayedain, F. S., "Nematicidal effect of cell-free culyure filtrates of EPN-symbiotic bacteria on Meloidogyne javanica", Biological Control of Pests & Plant Diseses, 8, pp.17~26 (2019)
  61. Stock, S. P., "Secondary metabolites produced by Heterorhabditis symbionts and their application in agriculture : what we know and what to do next", Journal of Nematology, 49, pp.373~383 (2017)
  62. Tigano, M. S., "Phylogeny of Meloidogyne spp. Based on 18S rDNA and the intergenic region of mitochondrial DNA sequences", Nematology, 7, pp.851~862 (2005)
  63. Tofangsazi, N., "Entomopathogenic nematodes", pp.2018, UF
  64. Vinardell, M. P., "Alternative methods for eye and skin irritation tests : an review", Journal of Pharmaceutical Sciences, 97, pp.46~59 (2008)
  65. Webster, J. M., "Entomopathogenic nematology" (2002), CABI Publishing
  66. Woodring, J. L., "Steinernematid and heterorhabditid nematodes: a handbook of techniques", Southern Cooperative Series Bulletin, 331, pp.30~ (1988), Arkansas Agricultural Experiment Station[DOI]

관련 논문AI추천

Toxicity Assessment of Photorhabdus temperata Isolated from Heterorhabditis megidis Gwangju Strain (Nematoda: Heterorhabditidae) in Fish and Rat김영준, 김진호, 박순한, 정남준, 추영무한국유기농업학회 · 한국유기농업학회지2022 · 제30권 제1호Antagonistic Efficacy of Symbiotic Bacterium Xenorhabdus sp. SCG against Meloidogyne spp.김종훈, Choi In-Soo, Koo Kyung-Bon, Lee Byeong-Min, Lee Hyung Chul, Son Kwang-Hee한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제8호Evaluation of Nematicidal Activity of Streptomyces yatensis KRA-28 against Meloidogyne incognita김강훈, 노문철, 박은재, 박찬선, 윤봉식, 이소영, 이승웅, 이승재, 장현재한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2020 · 제30권 제5호곤충살충성 세균 Photorhabdus의 Insecticidal Toxin과 연구동향신재호, 장은경한국미생물·생명공학회 · 한국미생물·생명공학회지2010 · 제38권 제2호Neobacillus sp. JC05 추출물을 처리한 오이 식물의 고구마뿌리혹선충에 대한 방어 반응 검정김유리, 상미경, 장화진한국유기농업학회 · 한국유기농업학회지2022 · 제30권 제3호Root-Knot Nematode (Meloidogyne incognita) Control Using a Combination of Lactiplantibacillus plantarum WiKim0090 and Copper Sulfate김진철, 박해웅, 장지윤, Jeong Seul-Gi, Kim Ho Myeong, Kim Seulbi, Park Ae Ran, Seo Hye Jeong, Yeon Jehyeong, Yu Nan Hee한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2022 · 제32권 제8호

이 간행물의 다른 논문 (6건)