Root-Knot Nematode (Meloidogyne incognita) Control Using a Combination of Lactiplantibacillus plantarum WiKim0090 and Copper Sulfate
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Lactic acid bacteria (LAB) exert antagonistic activity against root-knot nematodes, mainly by producing organic acids via carbohydrate fermentation. However, they have not yet been used for root-knot nematode (Meloidogyne incognita) control owing to a lack of economic feasibility and effectiveness. In this study, we aimed to isolate organic acid-producing LAB from kimchi (Korean traditional fermented cabbage) and evaluated their nematicidal activity. Among the 234 strains isolated, those showing the highest nematicidal activity were selected and identified as Lactiplantibacillus plantarum WiKim0090. Nematicidal activity and egg hatch inhibitory activity of WiKim0090 culture filtrate were dose dependent. Nematode mortality 3 days after treatment with 2.5% of the culture filtrate was 100%, with a 50% lethal concentration of 1.41%. In pot tests, the inhibitory activity of an L. plantarum WiKim0090-copper sulfate mixture on gall formation increased. Compared to abamectin application, which is a commercial nematicide, a higher control value was observed using the WiKim0090-copper sulfate mixture, indicating that this combination can be effective in controlling the root-knot nematode.
Abstract
Lactic acid bacteria (LAB) exert antagonistic activity against root-knot nematodes, mainly by producing organic acids via carbohydrate fermentation. However, they have not yet been used for root-knot nematode (Meloidogyne incognita) control owing to a lack of economic feasibility and effectiveness. In this study, we aimed to isolate organic acid-producing LAB from kimchi (Korean traditional fermented cabbage) and evaluated their nematicidal activity. Among the 234 strains isolated, those showing the highest nematicidal activity were selected and identified as Lactiplantibacillus plantarum WiKim0090. Nematicidal activity and egg hatch inhibitory activity of WiKim0090 culture filtrate were dose dependent. Nematode mortality 3 days after treatment with 2.5% of the culture filtrate was 100%, with a 50% lethal concentration of 1.41%. In pot tests, the inhibitory activity of an L. plantarum WiKim0090-copper sulfate mixture on gall formation increased. Compared to abamectin application, which is a commercial nematicide, a higher control value was observed using the WiKim0090-copper sulfate mixture, indicating that this combination can be effective in controlling the root-knot nematode.
참고문헌 (38)
- M. Moens, "Meloidogyne species - a diverse novel group and important plant parasites", Root-knot nematodes, pp.1~17 (2009), CABI[DOI]
- Milligan SB, "The root knot nematode resistance gene Mi from tomato is a member of the leucine zipper, nucleotide binding, leucine-rich repeat family of plant genes", Plant Cell, 10, pp.1307~1319 (1998)
- R. A. Sikora, "Nematode parasites of vegetables.", Plant parasitic nematodes in subtropical and tropical agriculture, pp.319~392 (2005), CABI Publishing[DOI]
- Choo HY, "Studies on the patterns of plastic film house, their growing conditions, and diseases and pests occurrence on horticultural crops in southern part of Korea. Insects and nematodes associated with horticultural crops and effect of nursery soil conditions on the infection of root-knot nematode", Korean J. Plant Prot, 26, pp.195~201 (1987)
- Manzanilla-Lopez RH, "Nematology Advances and Perspectives, Nematode Management and Utilization" (2004), CAB International
- Trudgill DL, "Apomictic, polyphagous root-knot nematodes: exceptionally successful and damaging biotrophic root pathogens", Annu. Rev. Phytopathol, 39, pp.53~77 (2001)
- 전한식, "성주 지역 시설참외 연작지의 토양특성 및 토양선충 변화", 한국환경농학회지, 20(2), pp.127~132 (2001), 한국환경농학회
- 이후관, "유채박 이용 시설하우스 연작재배 시 뿌리혹선충 밀도억제효과", 한국자원식물학회지, 28(1), pp.93~100 (2015), 한국자원식물학회[DOI]
- Kim TY, "Nematicidal activity of grammicin produced by Xylaria grammica KCTC 13121BP against Meloidogyne incognita", Pest Manag. Sci, 74, pp.384~391 (2018)
- HaeWoong Park, "Biocontrol potential of Myrothcium roridum Tode ex Fr. (Hypocreales: Incertae sedis) against root-knot nematode Meloidogyne incognita (Kofoid & White) Chitwood (Tylenchida: Heteroderidae)", Journal of Asia-Pacific Entomology, 19(2), pp.447~450 (2016), 한국응용곤충학회
- Hye Jeong Seo, "Biological Control of Root-Knot Nematodes by Organic Acid-Producing Lactobacillus brevis WiKim0069 Isolated from Kimchi", The Plant Pathology Journal, 35(6), pp.662~673 (2019), 한국식물병리학회[DOI]
- Desaeger J, "Methyl bromide alternatives for control of root-knot nematode (Meloidogyne spp.) in tomato production in Florida", J. Nematol, 49, pp.140~149 (2017)
- Bintsis T, "Lactic acid bacteria : their applications in foods", J. Bacteriol. Mycol, 6, pp.89~94 (2017)
- Hamed HA, "In vivo efficacy of lactic acid bacteria in biological control against Fusarium oxysporum for protection of tomato plant", Life Sci. J, 8, pp.462~468 (2011)
- Trias R, "Lactic acid bacteria from fresh fruit and vegetables as biocontrol agents of phytopathogenic bacteria and fungi", Int. Microbiol, 11, pp.231~236 (2008)
- Eloh K, "Potent nematicidal activity of maleimide derivatives on Meloidogyne incognita", J. Agric. Food Chem, 64, pp.4876~4881 (2016)
- Korthals GW, "Long-term effects of copper and pH on the nematode community in an agroecosystem", Environ. Toxicol. Chem, 15, pp.979~985 (1996)
- Yeon J, "Control of root-knot nematodes by a mixture of maleic acid and copper sulfate", Appl. Soil Ecol, 141, pp.61~68 (2019)
- Berg J, "Copper amendment of agricultural soil selects for bacterial antibiotic resistance in the field", Lett. Appl. Microbiol, 40, pp.146~151 (2005)
- McBride RG, "The role of low molecular weight organic acids from decomposing rye in inhibiting root-knot nematode populations in soil", Appl. Soil Ecol, 15, pp.243~251 (2000)
- Tabarant P, "Effects of four organic amendments on banana parasitic nematodes and soil nematode communities", Appl. Soil Ecol, 49, pp.59~67 (2011)
- Yan D, "Community structure of soil nematodes under different drought conditions", Geoderma, 325, pp.110~116 (2018)
- Bakonyi G, "Soil nematode community structure as affected by temperature and moisture in a temperate semiarid shrubland", Appl. Soil Ecol, 37, pp.31~40 (2007)
- 서윤희, "Control of Meloidogyne incognita Using Mixtures of Organic Acids", The Plant Pathology Journal, 30(4), pp.450~455 (2014), 한국식물병리학회[DOI]
- Park HW, "Biological control potentials of insect-parasitic nematode Rhabditis blumi(Nematoda : Rhabditida)for major cruciferous vegetable insect pests", Appl. Entomol. Zool, 47, pp.389~397 (2012)
- Terefe M, "Effect of a formulation of Bacillus firmus on root-knot nematode Meloidogyne incognita infestation and the growth of tomato plants in the greenhouse and nursery", J. Invertebr. Pathol, 100, pp.94~99 (2009)
- Cayrol J-C, "Study of the nematocidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus", Rev. Nematol, 12, pp.331~336 (1989)
- Schneider P, "Entomologisches Praktikum", pp.237~ (1947), Sauerländer Co
- Barker KR, "An advanced treatise on Meloidogyne", pp.19~35 (1985), North Carolina State University
- Jenkins WR, "A rapid centrifugal-flotation technique for separating nematodes from soil.", Plant Dis. Rep, 48, pp.692~ (1964)
- 조명래, "Comparison of Inoculation Methods, Meloidogyne spp. and Different Host Plants for Production of Pasteuria penetrans", Journal of Asia-Pacific Entomology, 8(3), pp.297~300 (2005), 한국응용곤충학회
- Takatsu T, "1100-50, a novel nematicide from Streptomyces lavendulae SANK 64297", J. Antibiot. (Tokyo), 56, pp.306~309 (2003)
- Wilson MJ, "Progress in the commercialization of bionematicides", BioControl, 58, pp.715~722 (2013)
- El-Mabrok ASW, "Screening of lactic acid bacteria as biocontrol against(Collectotrichum capsici)on chilli bangi", Res. J. Appl. Sci, 7, pp.446~473 (2012)
- Jang JY, "Biological control of Meloidogyne incognita by Aspergillus niger F22 producing oxalic acid", PLos One, 11, pp.e0156230~ (2016)
- Mendoza AR, "In vitro activity of Bacillus firmus against the burrowing nematode Radopholus similis, the root-knot nematode Meloidogyne incognita and the stem nematode Ditylenchus dipsaci", Biocontrol Sci. Technol, 18, pp.377~389 (2008)
- Shinya R, "Effects of fungal culture filtrates of Verticillium lecanii (Lecanicillium spp.) hybrid strains on Heterodera glycines eggs and juveniles", J. Invertebr. Pathol, 97, pp.291~297 (2008)
- Noy-Meir I, "Desert ecosystems : higher trophic levels", Annu. Rev. Ecol. Syst, 5, pp.195~214 (1974)
