B16 Melanoma 세포에서 Chitosan Oligosaccharide에 의한 Melanin 생성과 Tyrosinase 활성 및 발현과의 관계

Abstract

To investigate the effect of chitosn oligosaccharide on cosmetic and skin care, we measured tyrosinase activity and melanin production in B16 melanoma cells, and elastase and hyaluronidase activity. Chitosan oligosaccharide itself did not have any anti-oxidant activity in DPPH radical scavenging, and did not affect the proliferation of B16 melanoma cells. Chitosan oligosaccharide dose-depednetly increased melanin production in the absence or presence of MSH. However, chitosan oligosaccharide did not have any influence on the tyrosinase activity and tyrosinase expression in B16 melanoma cells. These results suggest that chitosan oligosaccharide-induced melanin production may be independent on tyrosinase in B16 melanoma cells. On the other hand, chitosan oligosaccharide increased neutrophil elastase activity but decreased hyaluronidase activity in a dose-dependent manner. From the above results, chitosan oligosaccharide dose-dependently appears to increase melanin production in B16 melanoma cells and inhibit hyaruronidase activity, suggesting that chitosan oligosaccharide may be used as sun-tan agent and water conservative agent.