토마토 잎 초임계 추출물의 피부 색소침착 억제 효과
Inhibition Effect of Tomato Leaves Supercritical Extracts on Skin Pigmentaion
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
In this study, we investigated inhibition effects of tomato leaves supercritical extracts on skin pigmentaion. The leaves of tomato (Lycopersicon esculentum) contains functional ingredients such as lycopene, lutein,chlorophyll, caffeic acid, and antioxidant studies prior showed a whitening effect in vitro. RT-PCR and westernblotting analysis, tomato leaves supercritical extract with 0.01mg/mL concentration showed the mostlyinhibited tyrosinase activity on B16F1 cell. Supercritical extract of leaves of tomato, tomato half of the colorantwas prepared at a concentration of 0.5% (T-0.5%), and 1% (T-1%), formed by UVB radiation in the Hr-1 miceusing a mexameter after coated four times once eight days to two days supercritical extract and measuredthe level of melanin. The 6 day, melanin levels were significantly reduced in the T-1% (332.14±24.91) ascompared to N.cont group (428.72±48.39). The 8 day, melanin levels were significantly decreased in T1%group (261.25±16.32) and T-0.5% group (282.32±26.43). After changing anatomy of the expression level ofMatrix metalloproteinase-2, 9 (MMP-2, MMP-9), were collected tissue sample application portion investigationand the expression level of MMP-2 and MMP-9 as compared with N.cont group expression levels weresignificantly decreased T-1% group (405.11±23.94) and T-1% group (421.54±32.81). As a result of theabove, the results of animal experiments using the HR-1 mice, supercritical extract of tomato leaves, it wasconfirmed that there is a inhibition effect of skin pigmentaion and moisturizing and whitening effect.
Abstract
In this study, we investigated inhibition effects of tomato leaves supercritical extracts on skin pigmentaion. The leaves of tomato (Lycopersicon esculentum) contains functional ingredients such as lycopene, lutein,chlorophyll, caffeic acid, and antioxidant studies prior showed a whitening effect in vitro. RT-PCR and westernblotting analysis, tomato leaves supercritical extract with 0.01mg/mL concentration showed the mostlyinhibited tyrosinase activity on B16F1 cell. Supercritical extract of leaves of tomato, tomato half of the colorantwas prepared at a concentration of 0.5% (T-0.5%), and 1% (T-1%), formed by UVB radiation in the Hr-1 miceusing a mexameter after coated four times once eight days to two days supercritical extract and measuredthe level of melanin. The 6 day, melanin levels were significantly reduced in the T-1% (332.14±24.91) ascompared to N.cont group (428.72±48.39). The 8 day, melanin levels were significantly decreased in T1%group (261.25±16.32) and T-0.5% group (282.32±26.43). After changing anatomy of the expression level ofMatrix metalloproteinase-2, 9 (MMP-2, MMP-9), were collected tissue sample application portion investigationand the expression level of MMP-2 and MMP-9 as compared with N.cont group expression levels weresignificantly decreased T-1% group (405.11±23.94) and T-1% group (421.54±32.81). As a result of theabove, the results of animal experiments using the HR-1 mice, supercritical extract of tomato leaves, it wasconfirmed that there is a inhibition effect of skin pigmentaion and moisturizing and whitening effect.
참고문헌 (29)
- 권승빈, "글리세린, 히알루론산, 실리콘 오일이 피부의 보습 및 경피수분손실량에 미치는 효과", 대한피부미용학회지, 11(4), pp.761~768 (2013), 대한피부미용학회
- 최석현, "토마토 품종 및 부위별과 토마토 가공제품의 ascorbic acid, lycopene, β-carotene과 α-carotene 함량 비교", 한국조리학회지, 17(4), pp.263~272 (2011), 한국조리학회
- 김민정, "호장근 추출물의 미백효과 및 자외선 차단효과 분석", 대한피부미용학회지, 10(4), pp.961~968 (2012), 대한피부미용학회
- 김은호, "헛개나무내의 Matrix Metalloproteinase-9 활성 억제제에 관한 연구", 분석과학, 24(2), pp.135~141 (2011), 한국분석과학회
- 김의수, "Fisetin의 항산화 활성 및 미용기능식품 소재로서의 가능성 고찰", 대한피부미용학회지, 10(3), pp.515~521 (2012), 대한피부미용학회
- 모정희, "황칠나무 잎 메탄올 추출물 및 분획물의 Tyrosinase 저해 및 Melanin 생성억제 활성", 대한피부미용학회지, 11(2), pp.275~280 (2013), 대한피부미용학회
- 박주아, "소리쟁이 추출물을 이용한 멜라닌 생성억제와 피부의 멜라닌 · 홍반 값의 변화도", 대한피부미용학회지, 10(2), pp.291~297 (2012), 대한피부미용학회[DOI]
- 오봉윤, "딸기 식물체 추출물의 미백 및 주름 개선 효과", 대한피부미용학회지, 11(5), pp.969~974 (2013), 대한피부미용학회
- 오용열, "배양 SK-MEL-3세포에 있어서 로즈마리 추출물의 항산화능 및 멜라닌화에 대한 영향", 대한피부미용학회지, 9(4), pp.1~10 (2011), 대한피부미용학회
- 유민정, "SK-MEL-2에서 상백피 추출물의 Tyrosinase 활성 억제 및 Melanin 생성 억제 효과", 대한피부미용학회지, 9(4), pp.1~12 (2011), 대한피부미용학회
- 윤영민, "자외선(Ultravilet)이 피부 및 피부세포 내 신호전달체계에 미치는 영향", 대한피부미용학회지, 11(3), pp.417~426 (2013), 대한피부미용학회
- 이만효, "국내 시판 과일 및 야채 주스의 항균, 항산화 및 항혈전 활성", 한국미생물·생명공학회지, 39(2), pp.146~152 (2011), 한국미생물·생명공학회
- 정상철, "천연소재로부터 피부효능을 갖는 피부미용식품의 개발동향", 대한피부미용학회지, 11(2), pp.203~212 (2013), 대한피부미용학회
- 한정선, "솔잎 발효액의 항산화 활성 및 멜라닌 생성 억제 효과", 대한피부미용학회지, 10(3), pp.619~624 (2012), 대한피부미용학회
- 형순희, "Hairless 마우스에서 치자 열수추출물의 피부 주름형성 억제효과", 대한피부미용학회지, 7(2), pp.123~136 (2009), 대한피부미용학회
- Chichili GR, "β-Carotene Conversion into Vitamin A in Human Retinal Pigment Epithelial Cells", Invest. Ophthalmol. Vis. Sci., 46, pp.3562~3569 (2005)
- Gaggioli C, "Microphthalmia-associated transcription factor (MITF) is required but is not sufficient to induce the expression of melanogenic genes", Pigment Cell Res, 16, pp.374~382 (2003)
- Gerster H, "The potential role of lycopene for human health", J. Am. Coll. Nutr., 16, pp.109~126 (1997)
- 황주영, "도인승기탕의 B16F10 세포주에서의 멜라닌 생성 및 유전자 발현 억제 효과", 생명과학회지, 22(3), pp.318~323 (2012), 한국생명과학회
- Johnson EJ, "The role of carotenoids in human healt", Nutr. Clin. Care, 5, pp.47~49 (2002)
- A-Young Kim, "Preventive Effects of Lycopene-Enriched Tomato Wine against Oxidative Stress in High Fat Diet-Fed Rats", Preventive Nutrition and Food Science, 16(2), pp.95~103 (2011), 한국식품영양과학회
- 김혜은, "The effects of Caffeoylserotonin on inhibition of melanogenesis through the downregulation of MITF via the reduction of intracellular cAMP and acceleration of ERK activation in B16 murine melanoma cells", BMB Reports, 45(12), pp.724~729 (2012), 생화학분자생물학회
- 이수연, "멜라노마 세포에서 당귀추출물의 MITF, TRP-1, TRP-2, tyrosinase mRNA 발현 억제 효과", 생명과학회지, 23(11), pp.1336~1341 (2013), 한국생명과학회
- McCawley LJ, "Matrix metalloproteinases: multifunctional contributors to tumor progression", Mol. Med. Today, 6, pp.149~156 (2000)
- 나현숙, "Effects of Tomato Extracts on Detergent-Induced Dry Skin in Rats", 생명과학회지, 18(4), pp.441~446 (2008), 한국생명과학회
- 나환식, "재배지역에 따른 농산물의 phytochemical 함량", 한국식품저장유통학회지, 20(4), pp.451~458 (2013), 한국식품저장유통학회
- Rao AV, "Carotenoids and human health", Pharmacol. Res., 55, pp.207~216 (2007)
- Rim JH, "Electrical measurement of moisturing effect on skin hydration and barrier function in psoriasis patients", Clin. Exp. Dermatol, 30, pp.409~413 (2005)
- Rizwan M, "Tomato paste rich in lycopene protects against cutaneous photodamage in humans in vivo: a randomized controlled trial", Br. J. Dermatol, 164, pp.154~162 (2011)