인간각질형성세포에서 알파망고스틴의 자외선B 의존적 산화적스트레스에 대한 세포보호 효과
Protective Effects of α-Mangostin on UVB-induced Oxidative Stress and Cellular Senescence
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One of subtypes in ultraviolet (UV), UVB, has been reported that the most powerful factors causing photoaging and DNA lesions. Finding and developing both cost-effective and efficient materials are the urgent issues to protect the skin from UVB-induced damages. Alpha-mangostin (α-mangostin), the first xanthone isolated from Garcinia mangostana (mangosteen), has been studied for anti-inflammation and antioxidant properties. The purpose of this study to investigate effects of α-mangostin in HaCaT, keratinocyte against UVB radiation. To evaluate effects of α-mangostin upon UVB-induced cytotoxic damages, watersoluble tetrazolium salt (WST-1) assay, flow Cytometry analysis, qunatitative real-time PCR (qRT-PCR), and senescence associated β-galactosidase assay (SA-β-gal assay) were performed. Pretreatment of α-mangostin was verified protective effects on UVB-induced proliferative restrain, apoptotic cell death and senescence, in HaCaT keratinocytes. These results indicated the protective effects of α-mangostin against UVB, and suggest as a cosmeceutical ingredient.
Abstract
One of subtypes in ultraviolet (UV), UVB, has been reported that the most powerful factors causing photoaging and DNA lesions. Finding and developing both cost-effective and efficient materials are the urgent issues to protect the skin from UVB-induced damages. Alpha-mangostin (α-mangostin), the first xanthone isolated from Garcinia mangostana (mangosteen), has been studied for anti-inflammation and antioxidant properties. The purpose of this study to investigate effects of α-mangostin in HaCaT, keratinocyte against UVB radiation. To evaluate effects of α-mangostin upon UVB-induced cytotoxic damages, watersoluble tetrazolium salt (WST-1) assay, flow Cytometry analysis, qunatitative real-time PCR (qRT-PCR), and senescence associated β-galactosidase assay (SA-β-gal assay) were performed. Pretreatment of α-mangostin was verified protective effects on UVB-induced proliferative restrain, apoptotic cell death and senescence, in HaCaT keratinocytes. These results indicated the protective effects of α-mangostin against UVB, and suggest as a cosmeceutical ingredient.
참고문헌 (23)
- Balunas MJ, "Xanthones from the botanical dietary supplement mangosteen (Garcinia mangostana) with aromatase inhibitory activity", J. Nat. Prod, 71, pp.1161~1166 (2008)
- Campbell C, "Wavelength specific patterns of p53 induction in human skin following exposure to UV radiation", Cancer Res, 53, pp.2697~2699 (1993)
- Chairungsrilerd N, "Histaminergic and serotonergic receptor blocking substances from the medical plant Garcinia mangostatna", Planta Med, 62, pp.471~472 (1996)
- Chiou, WL, "Linear correlation of the fraction of oral dose absorbed of 64 drugs between humans and rats", Pharm. Res, 15, pp.1792~1795 (1998)
- Chomnawang MT, "Effect of Garcinia mangostana on inflammation caused by Propionibacterium acnes", Fitoterapia, 78, pp.401~408 (2000)
- Diffey BL, "What is light?", Photodermatol. Photoimmunol. Photomed, 18, pp.68~74 (2002)
- Ee GC, "Artomandin, a new xanthone from Artocarpus kemando (Moraceae)", Nat. Prod. Res, 10, pp.995~1003 (2011)
- Elsner P, "Anti-aging data and support claims—consensus statement", J. Dtsch. Dermatol. Ges, 9(Suppl. 3), pp.S1~S32 (2011)
- Farage MA, "Intrinsic and extrinsic factors in skin ageing: a review", Int. J. Cosmet. Sci, 30, pp.87~95 (2008)
- Han QB, "Caged Garcinia xanthones: development since 1937", Curr. Med. Chem, 16, pp.3775~3796 (2009)
- Hashizume H., "Skin aging and dry skin", J. Dermatol, 31, pp.603~609 (2004)
- Jenkins G., "Molecular mechanisms of skin ageing", Mech. Ageing Dev, 123, pp.801~810 (2002)
- Jung HA, "Antioxidant xanthones from the pericarp of Garcinia mangostana (mangosteen)", J. Agric. Food Chem, 54, pp.2077~2082 (2006)
- Krajarng A, "Antiproliferative effect of alpha-mangostin on canine osteosarcoma cells", Res. Vet. Sci, 93, pp.788~794 (2012)
- Landau M., "Exogenous factors in skin aging", Curr. Prob. Dermatol, 35, pp.1~13 (2007)
- Lee KS, "Troxerutin induces protective effects against ultraviolet B radiation through the alteration of microRNA expression in human HaCaT keratinocyte cells", Int. J. Mol. Med, 33, pp.934~942 (2014)
- Li P, "Alpha-mangostin inhibits intracellular fatty acid synthase and induces apoptosis in breast cancer", Mol. Cancer, 13, pp.138~ (2014)
- Matsumura Y, "Toxic effects of ultraviolet radiation on the skin", Toxicol. Appl. Pharmacol, 195, pp.298~308 (2004)
- Pedraza-Chaverrí J, "ROS scavenging capacity and neuroprotective effect of α-mangostin against 3-nitropropionic acid in cerebellar granule neurons", Exp. Toxicol. Pathol, 61, pp.491~501 (2008)
- Pothitirat W, "Antiacne-inducing bacterial activity of mangosteen fruit rind extracts", Med. Princ. Pract, 19, pp.281~286 (2010)
- Viña J, "Theories of ageing", IUBMB Life, 59, pp.249~254 (2007)
- Yesennia SP, "The α-mangostin prevention on cisplatin-induced apoptotic death in LLCPK1 cells is associated to an inhibition of ROS production and p53 induction", Chemico-Biological Interactions, 188, pp.144~150 (2010)
- Zouboulis CC, "Clinical aspects and molecular diagnostics of skin aging", Clin. Dermatol, 29, pp.3~14 (2011)