자외선 조사에 의해 노화된 인간각질형성세포에서 sulfuretin의 항노화 효능
Anti-aging Effect of Sulfuretin in UVA-irradiated Normal Human Epidermal Keratinocytes
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목적: 본 연구는 sulfuretin이 자외선에 의해 광노화가 유도된 인간각질형성세포의 항노화 효능을 확인하기 위하여 수행되었다. 방법: 자외선으로 노화를 유도한 인각각질형성세포에서 sulfuretin의 보습효과를 확인하기 위하여 세포생존율, 보습에 관련된 유전자발현양상, 히알루론산 단백질의 발현 정도를 확인하였다. 결과: Sufuretin의 항노화 효능을 확인하기 위하여 AQP3, HAS2, FLG, KRT1, KRT10의 유전자 발현을 확인한 결과, 자외선 조사에 의해 감소한 AQP3와 HAS2 유전자의 발현이 sulfuretin에 의해 증가하였다. 인각각질형성세포의 과각질화를 유도하는 KRT1과 KRT10과 과분화의 표지인자인 FLG의 발현이 sufuretin에 의해 감소하였다. 또한 히알루론산 단백질의 생성이 sulfuretin에 의해 증가함을 확인하였다. 결론: 본 연구를 통해 sulfuretin의 항노화 효능을 확인하였고, 향후 sulfuretin이 화장품 및 의약품과 건강식품의 개발에 활용할 수 있는 소재로서의 가능성을 확인하기 위해 심도있는 추가연구가 필요할 것으로 사료된다.
Abstract
Purpose: This study was carried out to investigate the epidermal moisturizing effects of sulfuretin on normal human epidermal keratinocytes (NHEKs). Methods: Epidermal moisturizing effects of sulfuretin on NHEKs were assessed by quantitative real-time RT-PCR to monitor the expression of genes related to skin hydration, hyaluronic acid (HA)-ELISA assays to detect HA production, and cell viability assays. Results: Sulfuretin increased the mRNA levels of the AQP3/HAS2/KRT1/KRT10 genes and HA production in NHEKs. Conclusion: This study led to the identification of the epidermal moisturizing effects of sulfuretin. The results indicate that sulfuretin can be a potent cosmetic ingredient for skin moisturizing and antiaging products. However, further research is warranted regarding the use of sulfuretin to develop not only cosmetics but also medicines and food.
참고문헌 (23)
- Assefa Z, "Ultraviolet radiation-induced apoptosis in keratinocytes : on the role of cytosolic factors", Biochimica et Biophysica Acta, 1755, pp.90~106 (2005)
- Bickers DR, "Oxidative stress in the pathogenesis of skin disease", Journal of Investigative Dermatology, 126, pp.256~275 (2006)
- Cao C, "All-trans retinoic acid attenuates ultraviolet radiation-induced down-regulation of aquaporin-3 and water permeability in human keratinocytes", Journal of Cellular Physiology, 215, pp.506~516 (2008)
- Choi J, "Antirheumatoid arthritis effect of Rhus verniciflua and of the active component, sulfuretin", Planta Medica, 69, pp.899~904 (2003)
- Eckert RL, "Transglutaminase function in epidermis", Journal of Investigative Dermatology, 124, pp.481~492 (2005)
- Fisher GJ, "Mechanisms of photoaging and chronological skin aging", Archives of Dermatology, 138, pp.1462~1470 (2002)
- Fuchs E, "Scratching the surface of skin development", Nature, 22, pp.834~842 (2007)
- Hara-Chikuma M, "Roles of aquaporin-3 in the epidermis", Journal of Investigative Dermatology, 128, pp.2145~2151 (2008)
- Hašová M, "Hyaluronan minimizes effects of UV irradiation on human keratinocytes", Archives of Dermatological Research, 303, pp.277~284 (2011)
- Jones DL, "Emerging models and paradigms for stem cell ageing", Nature Cell Biology, 13, pp.506~512 (2011)
- Karvinen S, "Keratinocyte growth factor stimulates migration and hyaluronan synthesis in the epidermis by activation of keratinocyte hyaluronan synthases 2 and 3", The Journal of Biological Chemistry, 278, pp.49495~49504 (2003)
- Kim J, "Inhibition effect of Gynura procumbens extract on UV-B-induced matrixmetalloproteinase expression in human dermal fibroblasts", Journal of Ethnopharmacology, 137, pp.427~433 (2011)
- Kim S, "Compound K induces expression of hyaluronan synthase 2 gene in transformed human keratinocytes and increases hyaluronan in hairless mouse skin", Biochemical and Biophysical Research Communication, 316, pp.348~355 (2004)
- Kirkwood TB, "Understanding the odd science of aging", Cell, 120, pp.437~447 (2005)
- Lee DS, "The cytoprotective effect of sulfuretin against tert-butyl hydroperoxide-induced hepatotoxicity through Nrf2", ARE and JNK, International Journal of Molecular Sciences, pp.2014, 19[DOI]
- Lee KW, "Sulfuretin from heartwood of Rhus verniciflua triggers apoptosis through activation of Fas, Caspase-8, and the mitochondrial death pathway in HL-60 human leukemia cells", Journal of Cellular Biochemistry, 113, pp.2835~2844 (2012)
- Lee YR, "Sulfuretin, a major flavonoid isolated from Rhus verniciflua, ameliorates experimental arthritis in mice", Life Science, 90, pp.799~807 (2012)
- 박선영, "자외선 조사에 의해 노화된 HaCaT 세포에서 보골지 추출물의 항노화 효능", 아시안뷰티화장품학술지, 14(2), pp.119~126 (2016), 한국피부과학연구원[DOI]
- 심중현, "자외선 조사에 의해 노화된 인간각질형성세포에서 감잎 추출물의 항노화 효능", 아시안뷰티화장품학술지, 15(2), pp.170~178 (2017), 한국피부과학연구원[DOI]
- 심중현, "인간 진피유래 줄기세포 배양액에 의한 인각각질형성세포의 노화 개선 효과", 아시안뷰티화장품학술지, 16(1), pp.42~51 (2018), 한국피부과학연구원[DOI]
- 심중현, "제아잔틴에 의한 RAW264.7 세포에서의 항염효과", 아시안뷰티화장품학술지, 17(4), pp.431~439 (2019), 한국피부과학연구원[DOI]
- Sougrat R, "Functional expression of AQP3in human skin epidermis and reconstructed epidermis", The Journal of Investigative Dermatology, 118, pp.678~685 (2002)
- Talwar HS, "Reduced type I and type III procollagens in photodamaged adult human skin", The Journal of Investigative Dermatology, 105, pp.285~290 (1995)