Palmitoyl Teterapeptide-7 Gel이 PM10에 의해 유발된 염증 반응 제어 효과
Control Effect of Palmitoyl Teterapeptide-7 Gel to Inflammatory Responses Elicited by PM10
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목적: 최근 환경오염이 미치는 인체 유해성에 대한 인식이 높아지면서 미세먼지 차단뿐 아니라 미세먼지로 인한 피부 손상을 줄이고 회복시킬 수 있는 제품 개발이 요구되고 있다. 따라서 이 연구에서는 palmitoyl tetrapeptide-7 (PT-7)을 이용하여 화장품을 제조하고 안정성 및 안전성, 항오염 효능을 평가하였다. 방법: 항오염 화장품은 항염증 효능이 예상되는 PT-7을 사용하여 3% PT-7 gel, 5% PT-7 gel을 제조하였다. 화장품 안전성 측면에서, 20-50대의 피부질환 및 피부 과민 반응이 없는 성인을 대상으로 인체 적용에 대한 피부자극반복패치테스트(human repeat insult patch test)를 실시하였다. 제조한 항오염 화장품의 효능을 평가를 위해hairless 마우스 등 피부에 PM10을 일주일간 노출시킨 후 3% PT-7 gel, 5% PT-7 gel을 4주 간 도포하였다. 마우스 등 피부를 채취하여 PM10에 의해 발현된 IL-1β, IL-6의 억제 정도를 immunohistochemistry (IHC)와 western blot을 이용하여 확인하였다. 결과: 인체적용에 대한 안전성 평가 결과, 3% PT-7 gel과 5% PT-7 gel 모두 음성 (-) 반응이 나타나 안전성을 확인하였다. 항염증 효능평가를 위해 IHC의 영상을 판독한 결과 PM10 처치 후 3% PT-7 gel과 5% PT-7 gel 도포 군 모두 PM10 처치 군에 비해 IL-1β, IL-6의 발현이 감소하였다. Western blot 결과, 3% PT-7 gel과 5% PT-7 gel 도포 군 모두 PM10 노출에 의한 IL-1β, IL-6의 발현을 억제되었으며, 특히 5% PT-7 gel의 경우 IL-1β, IL-6의 발현을 현저히 억제되어 통계적으로 유의한 차이를 보였다. 결론: 이상의 결과를 종합하면 PT-7을 함유한 항오염 화장품은 PM10 노출에 의해 유발된 염증성 사이토카인 IL-6, IL-1β의 발현을 감소시키는 것을 확인하였다. 따라서 PT-7은 미세먼지로 인한 피부손상 예방 또는 개선을 위한 기능성 제품으로의 활용을 기대할 수 있다.
Abstract
Purpose: As a cosmetic approach to prevent pollution-induced skin damage, the topical application of skincare products, infused with functional ingredients, can offer an optimal solution to skin problems. The study aims to assess the anti-inflammatory effect of PT-7 gel. Methods: This study prepared antipollution cosmetics using palmitoyl tetrapeptide-7 (PT-7; 3% PT-7 gel, 5% PT-7 gel) and performed safety testing. For the safety assessment, the human repeat insult patch test (HRIPT) was performed to detect erythema occurrence on the patch area, following the ICDRG criteria. The panels comprised of 30 adults, aged 20–50 years. Furthermore, this study also analyzed particulate matter (PM) 10-induced inflammation on the skin of a hairless mouse by immunohistochemistry and Western blot. Results: The cosmetic safety testing results showed no skin reactions to antipollution gels containing PT- 7. Immunohistochemistry and the Western blot analysis demonstrated reduced levels of IL-1β and IL-6 expression levels in the 3% PT-7 and 5% PT-7 application groups after PM10 treatment. In particular, after PM10 treatment, the IL-1β and IL-6 expression levels were statistically significantly decreased in the group treated with 5% PT-7. Conclusions: Collectedly, this study provided evidence of the PT-7 gel’s safety and found that PT-7 gel reduced the IL-1β and IL-6 expression levels caused by PM10 exposure. In view of these results, it supports that PT-7 is a promising antipollution cosmetic ingredient with great commercial potential.
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