In Vitro Biological Activities and Relative Chemicals in Different Extracts of Licorice and Fermenting Licorice
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: This paper aims to qualitatively and quantitatively analyze the differences between the main chemicals in different licorice and fermented licorice (FL) extracts and their in vitro skin care activities and active ingredients. To evaluate the effect of fermentation on the efficacy of licorice. Methods: The DPPH free radical scavenging activity, mushroom tyrosinase activity, tyrosinase activity of B16F10 cells, and melanin synthesis inhibition activity of different extracts treated by fermentation and different extracts without fermentation treatment were evaluated. The effects of ethyl acetate components (EaF) including glycyrrhizin, isoglycyrrhizin, liquiritoside, isoliquiritigenin and glycyrrhizin ketone A on melanin synthesis were tested in vitro. Further assessment of FL at stromal metalloproteinase-1 (MMP-1) levels in human dermal fibroblasts (HDF). Results: Glycyrrhizin, isoglycyrrhizin, liquiritoside, isoliquiritigenin were improved in FL, and the antioxidant and MMP-1 inhibitory activities of FL controls were significantly increased. Conclusion: After fermentation, the anti-aging effect of licorice was greatly improved, indicating that the fermentation process had a good effect on improving the efficacy of licorice.
Abstract
Purpose: This paper aims to qualitatively and quantitatively analyze the differences between the main chemicals in different licorice and fermented licorice (FL) extracts and their in vitro skin care activities and active ingredients. To evaluate the effect of fermentation on the efficacy of licorice. Methods: The DPPH free radical scavenging activity, mushroom tyrosinase activity, tyrosinase activity of B16F10 cells, and melanin synthesis inhibition activity of different extracts treated by fermentation and different extracts without fermentation treatment were evaluated. The effects of ethyl acetate components (EaF) including glycyrrhizin, isoglycyrrhizin, liquiritoside, isoliquiritigenin and glycyrrhizin ketone A on melanin synthesis were tested in vitro. Further assessment of FL at stromal metalloproteinase-1 (MMP-1) levels in human dermal fibroblasts (HDF). Results: Glycyrrhizin, isoglycyrrhizin, liquiritoside, isoliquiritigenin were improved in FL, and the antioxidant and MMP-1 inhibitory activities of FL controls were significantly increased. Conclusion: After fermentation, the anti-aging effect of licorice was greatly improved, indicating that the fermentation process had a good effect on improving the efficacy of licorice.
참고문헌 (31)
- Ablise M, "Isolation and antioxidant property of glabridin", Natural Product Research & Development, 19, pp.675~682 (2007)
- Chang CC, "Estimation of total flavonoid content in propolis by two complementary colorimetric methods", Journal of Food and Drug Analysis, 10, pp.178~182 (2002)
- Chen DB, "Protective effect of glycyrrhizic flavone on hairless skin of mice irradiated by ultraviolet rays", Journal of Yichun Universy, pp.17~19 (2014)
- Chen YQ, "Microbial transformation of liquorice to enhance the pharmaceutical effects", Journal of Sichuan University(Natural Science Edition), 44, pp.1147~1150 (2007)
- Chin YW, "Anti-oxidant constituents of the roots and stolons of licorice(Glycyrrhiza glabra)", Journal of Agricultural and Food Chemisty, 55, pp.4691~4697 (2007)
- Cui YM, "Study on antioxidantive effects of total flavonoids of Glycyrrhiza on edible oils", Food Science, 28, pp.119~ (2007)
- Fang DJ, "Determination of total carbohydrate and uronic acid contents in polysaccharides from Poria cocos", Information on Traditional Chinese Medicine, 28, pp.42~44 (2011)
- Fu Y, "Antioxidant and antiinflammatory activities of six flavonoids separated from licorice", Food Chemisty, 141, pp.1063~1071 (2013)
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Research, 45, pp.1474~1478 (1985)
- Hsu YL, "Isoliquiritigenin inhibits the proliferation and induces the apoptosis of human nonsmall cell lung cancer a549 cells", Clinical Experimental Pharmacology & Physiology, 31, pp.414~418 (2004)
- Kolbe L, "Anti-inflammatory efficacy of licochalcone A : correlation of clinical potency and in vitro effects", Archives of Dermatological Research, 298, pp.23~30 (2006)
- Kwon HM, "Blockade of cytokine-induced endothelial cell adhesion molecule expression by licorice isoliquiritigenin through NF-kappaB signal disruption", Experimetal Biology and Medicine(Maywood), 232, pp.235~245 (2007)
- Lee CK, "Effects of the licorice extract against tumor growth and cisplatin-induced toxicity in a mouse xenograft model of colon cancer", Biological & Pharmaceutical Bulletin, 30, pp.2191~2195 (2007)
- Lee MH, "Bioactive constituents of Spatholobus suberectus in regulating tyrosinase-related proteins and mRNA in HEMn cells", Phytochemistry, 67, pp.1262~1270 (2006)
- Li Y, "Microbial fermentation promote extracting rate of flavonoids from Glycyrrhiza residues", Food Reserch and Development, 31, pp.156~159 (2010)
- Lu C, "Synthesis of lipoic acid-peptide conjugates and their effect on collagen and melanogenesis", European Journal of Medicinal Chemistry, 69, pp.449~454 (2013)
- Ma JB, "Effects of glabridin on B16 Cell metabolism", Journal of Shanghai Meidca, 30, pp.353~355 (2003)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays", Journal of Immunologicla Methods, 65, pp.55~63 (1983)
- Rahman AU, "Bioassay techniques for drug development", pp.14~16 (2010), Harwood Academic
- Simmler C, "Phytochemistry and biological properties of glabridin", Fitoterapia, 90, pp.160~184 (2013)
- Singleton VL, "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid regeants", American Journal of Enology and Viticulture, 16, pp.144~158 (1965)
- Song NR, "Licochalcone A, a polyphenol present in licorice, suppresses UV-induced COX-2 expression by targeting PI3K, MEK1, and B-Raf", International Journal of Molecular Sciences, 16, pp.4453~4470 (2015)
- Takei M, "Glycyrrhizin inhibits the manifestations of antiinflammatory responses that appear in association with systemic inflammatory response syndrome(SIRS)-like reactions", Cytokine, 35, pp.295~301 (2006)
- Wang ML, "Clinical observation on the therapeutic effect of licorice flavonoids in the treatment of yellow brown spot", Liaoning Pharmacy and Clinical Remedies, 3, pp.128~129 (2003)
- Wang YC, "Simultaneous quantification of flavonoids and triterpenoids in licorice using HPLC", Journal of Chromatography B, Analyticle Technologies in the Biomedical and Life Sciences, 850, pp.392~399 (2007)
- Xie YC, "Inhibitory effects of flavonoids extracted from licorice on lipopolysaccharideinduced acute pulmonary inflammation in mice", International Immunopharmacology, 9, pp.194~200 (2009)
- Xu L, "Skin-whitening", anti-freckle cosmetics and its future development, 2001, pp.31, 42~45
- Ye HY, "Study on effect of antisenility of liquorice flavone", Journal of Harbin University of Commerce(Natural Sciences Edition), 2, pp.20~21 (2004)
- Yokota T, "The inhibitory effect of glabridin from licorice extracts on melanogenesis and inflammation", Pigment Cell Research, 11, pp.355~361 (1998)
- Zhang Q, "Chemical analysis of the Chinese herbal medicine Gan-Cao(licorice)", Journal of Chromatogr A, 1216, pp.1954~1969 (2009)
- Zhao SY, "Experimental study on the in vivo antitumor effect of the total flavonoids from radix glycyrrhizae", Guangxi Medical Journal, 9, pp.1348~1349 (2006)