만성 염증성 질환 치료를 위한 원적외선 요법의 소재로서 Elixir StoneTM의 생리활성 평가
Assessment of the Bioactivity of Elixir Stone™ as a Far-Infrared Therapeutic Agent for Treating Chronic Inflammatory Diseases
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초록
목적: 본 연구는 마우스 대식세포와 인간 각질형성세포의 LPS 유도 과염증 모델에서, 원적외선 방사 특성을 가진 Elixir StoneTM의 항염, 항통증 및 상처 치유 효능을 규명함으로써 만성 염증성 질환 치료 소재로의 활용 가능성을 검토하였다. 방법: LPS 처리를 통해 염증 반응을 유도하였으며, Elixir StoneTM Patch의 염증성 사이토카인 및 통증 관련 인자에 대한 영향을 평가하기 위해 역전사 중합효소 연쇄 반응을 통한 유전자 분석과 NO assay, ELISA를 활용하였다. 또한 형질감염을 통해 NF-κB의 활성에 대한 영향을 평가하였고, wound healing assay를 통해 상처 치유 능력을 검증하였다. 결과: Elixir StoneTM과 Elixir StoneTM Patch는 우수한 원적외선 방출 성능을 나타내는 것으로 확인되었다. Elixir StoneTM Patch는 염증성 사이토카인과 염증매개물질인 산화 질소 생성을 효과적으로 감소시켰으며, NF-κB가 핵으로 이동하는 것을 억제하여 본 신호전달 경로의 활성화를 차단하였다. 또한 통증을 유도하는 인자인 COX-2와 PGE2의 발현을 감소시켰고, 상처 치유 기능을 개선함을 확인하였다. 결론: 본 연구는 Elixir StoneTM이 LPS로 유도된 염증 반응에서 염증 및 통증을 효과적으로 완화하고 상처 치유 능력을 개선함을 확인하였으며, 만성 염증성 질환 치료를 위한 기능성 경피 패치 소재로의 활용 가능성을 제시하였다.
Abstract
Purpose: This study aimed to evaluate the potential of Elixir StoneTM as a therapeutic material against chronic inflammatory diseases by testing its anti-inflammatory, pain-relieving, and wound-healing effects in lipopolysaccharide (LPS)-induced hyper-inflammation models. Methods: Hyper-inflammatory responses were induced via LPS treatment. The effects of Elixir StoneTM Patch on inflammatory cytokines and pain-related factors were analyzed using reverse transcription polymerase chain reaction to determine gene expression levels as well as using the nitric oxide (NO) and enzyme-linked immunosorbent assays. The impact of nuclear factor kappa B (NF-κB) activation was assessed via plasmid transfection, while the wound-healing capacity was examined by evaluating cell migration. Results: Elixir StoneTM and Elixir StoneTM Patch demonstrated excellent far-infrared emission properties. Elixir StoneTM Patch significantly reduced the production of inflammatory cytokines and NO, which are key mediators of inflammation. In addition, it inhibited the nuclear translocation of NF-κB, thereby suppressing the activation of this signaling pathway. Elixir StoneTM Patch also decreased the expression of pain-related factors cyclooxygenase-2 and prostaglandin E2, while enhancing wound-healing abilities. Conclusion: The results of this study confirm that Elixir StoneTM Patch is effective in alleviating LPS-induced chronic inflammation and pain in addition to promoting wound healing. These findings suggest the utility of the Elixir StoneTM as a functional transdermal patch material to treat chronic inflammatory diseases.
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