石決明의 抗炎症效果
Anti-Inflammatory Effects of Haliotidis Concha
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Objectives : Haliotidis Concha has been used to treat various human diseases such as liver dysfunction and inflammatory disorder. Although it has been shown the effects of Haliotidis Concha on the various diseases, it has almost not been studied about the anti-inflammatory effects of the Haliotidis Concha and its mechanisms. Methods : This research investigated the effects of the Haliotidis Concha ethanol extract (HCE) on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) as well as tumor necrosis factor-alpha (TNF-α). The protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were assayed by immunoblot analyses, and the productions of NO, PGE2 and TNF-α were assessed by ELISA. Results : Haliotidis Concha decreased the production of NO and PGE₂, and inhibited the expression iNOS and COX-2 proteins in a concentration-dependent manner in LPS-treated Raw 264.7 cells. HCE suppressed the ability of LPS to activate the signaling pathways of nuclear factor kappa B (NF-κB) as indicated by HCE inhibited nuclear NF-κB level and I-κBα phosphorylation. Also, HCE inhibited mitogen-activated protein kinases (MAPKs). Conclusions : HCE repressed the production of LPS-inducible NO, PGE₂ and TNF-α, which may be mediated by inhibition of NF-κB translocation. This study suggest the use for the treatment of acute inflammatory disorders.
Abstract
Objectives : Haliotidis Concha has been used to treat various human diseases such as liver dysfunction and inflammatory disorder. Although it has been shown the effects of Haliotidis Concha on the various diseases, it has almost not been studied about the anti-inflammatory effects of the Haliotidis Concha and its mechanisms. Methods : This research investigated the effects of the Haliotidis Concha ethanol extract (HCE) on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) as well as tumor necrosis factor-alpha (TNF-α). The protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were assayed by immunoblot analyses, and the productions of NO, PGE2 and TNF-α were assessed by ELISA. Results : Haliotidis Concha decreased the production of NO and PGE₂, and inhibited the expression iNOS and COX-2 proteins in a concentration-dependent manner in LPS-treated Raw 264.7 cells. HCE suppressed the ability of LPS to activate the signaling pathways of nuclear factor kappa B (NF-κB) as indicated by HCE inhibited nuclear NF-κB level and I-κBα phosphorylation. Also, HCE inhibited mitogen-activated protein kinases (MAPKs). Conclusions : HCE repressed the production of LPS-inducible NO, PGE₂ and TNF-α, which may be mediated by inhibition of NF-κB translocation. This study suggest the use for the treatment of acute inflammatory disorders.
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