바이오 교육훈련을 위한 Rituximab 발현 재조합 CHO 세포주의 특성 분석
Characterization of Recombinant Rituximab-Producing CHO Cells for Biotechnical Education and Training
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In this study, we investigated the growth and production characteristics of a Recombinant rituximab-producing CHO cell line in an economic, serum-free medium. The cell line showed a typical cell growth curve with high viability in a 25 mL Erlenmeyer flask suspension culture. The maximum expression level of Rituximab was 224.3 mg/L, which increased in proportional to the number of viable cells over the culture time. SDS-PAGE analysis under non-reducing and reducing conditions showed that the recombinant cell line secreted Rituximab with a predicted molecular weight, having the proper heavy and light chains. In addition, the production level was high enough to be detected on the SDS-PAGE by Coomassie staining. To investigate the feasibility of the cell culture supernatant as a component of biocosmetic products, both a cream formulation containing the cell culture supernatant and a serum-free control medium were prepared. without any visible disturbance, so that this recipe could be utilized for biocosmetic experiment education. Taken together, this study suggests that the presented recombinant CHO cell line could be a useful experimental tool in bioprocess and biocosmetic technology education and training.
Abstract
In this study, we investigated the growth and production characteristics of a Recombinant rituximab-producing CHO cell line in an economic, serum-free medium. The cell line showed a typical cell growth curve with high viability in a 25 mL Erlenmeyer flask suspension culture. The maximum expression level of Rituximab was 224.3 mg/L, which increased in proportional to the number of viable cells over the culture time. SDS-PAGE analysis under non-reducing and reducing conditions showed that the recombinant cell line secreted Rituximab with a predicted molecular weight, having the proper heavy and light chains. In addition, the production level was high enough to be detected on the SDS-PAGE by Coomassie staining. To investigate the feasibility of the cell culture supernatant as a component of biocosmetic products, both a cream formulation containing the cell culture supernatant and a serum-free control medium were prepared. without any visible disturbance, so that this recipe could be utilized for biocosmetic experiment education. Taken together, this study suggests that the presented recombinant CHO cell line could be a useful experimental tool in bioprocess and biocosmetic technology education and training.
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