해수로부터 Urease 활성 Photobacterium sp. Strain HA-2 분리
Isolation of Urease-Positive Photobacterium sp. Strain HA-2 from Sea Water
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Urease catalyzes the hydrolysis of urea into ammonia and carbamate. The latter spontaneously decomposes into another ammonia molecule and carbonic acid. We isolated a urease-positive bacterium from seawater on the western coast of Gomso bay in Korea. Using biochemical, morphological, and 16S rRNA gene analyses, we identified the bacterium as a P hotobacterium sp. and named it P hotobacterium sp. strain HA-2. We observed that the P hotobacterium sp. strain HA-2 showed a 2.0-fold difference in urease activity, when cultured in an LB medium containing 3% NaCl with or without 0.1% urea (0.1620 vs. 0.0820 μmol NH3min/mgprotein) In this study, we also cloned and sequenced a ca. 15-kb DNA fragment from the chromosome of P hotobacterium sp. strain HA-2, containing the urease gene clusters. We found ten open reading frames (ORFs) and the GC-content (40.9%) showed almost a perfect resemblance to the total genome of P hotobacterium damselae subsp. damselae (40%). The urease gene cluster consisted of seven genes, and its genetic organization and sequences resembled those found in Vibrio parahaemolyticus and Vibrio fischeri.
Abstract
Urease catalyzes the hydrolysis of urea into ammonia and carbamate. The latter spontaneously decomposes into another ammonia molecule and carbonic acid. We isolated a urease-positive bacterium from seawater on the western coast of Gomso bay in Korea. Using biochemical, morphological, and 16S rRNA gene analyses, we identified the bacterium as a P hotobacterium sp. and named it P hotobacterium sp. strain HA-2. We observed that the P hotobacterium sp. strain HA-2 showed a 2.0-fold difference in urease activity, when cultured in an LB medium containing 3% NaCl with or without 0.1% urea (0.1620 vs. 0.0820 μmol NH3min/mgprotein) In this study, we also cloned and sequenced a ca. 15-kb DNA fragment from the chromosome of P hotobacterium sp. strain HA-2, containing the urease gene clusters. We found ten open reading frames (ORFs) and the GC-content (40.9%) showed almost a perfect resemblance to the total genome of P hotobacterium damselae subsp. damselae (40%). The urease gene cluster consisted of seven genes, and its genetic organization and sequences resembled those found in Vibrio parahaemolyticus and Vibrio fischeri.
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