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국화 꽃색 변경을 위한 플라보노이드 대사공학

Flavonoid Metabolic Engineering for Modification of Flower Color in Chrysanthemum

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초록

In ornamental crops, the color and shape of flowers are one of the important traits. Generally, flower colors are determined by accumulating pigments such as carotenoids, flavonoids, and betalains. Among them, flavonoids are responsible for broad ranges of colors. Chrysanthemums are one of the most popular ornamental crops in the world, and there have been many efforts to change their flower color. In chrysanthemum flowers, cyanidin-based anthocyanin confers pink or red color, whereas terpenoid-based carotenoids are mainly responsible for yellow and green colors. However, blue colored chrysanthemums do not occur in nature. To date, there have been attempts to obtain blue or violet-colored chrysanthemum flowers through the introduction of a novel gene for accumulating delphinidin-based anthocyanins, while other studies have reported changing endogenous metabolites through the reconstruction of flavonoid biosynthesis. Since various transcription factors are involved in the regulation of flavonoid biosynthesis, it is important to understand not only the structural genes, but also the transcription factors required for the modification of flavonoid-based flower color. Therefore, in this paper, we describe the flavonoid biosynthetic pathway and its regulation, and review previous studies on the change in flower color through modification of flavonoid biosynthesis. This effort could be an important milestone in successfully achieving the modification of chrysanthemum flower color by means of plant biotechnology.

Abstract

In ornamental crops, the color and shape of flowers are one of the important traits. Generally, flower colors are determined by accumulating pigments such as carotenoids, flavonoids, and betalains. Among them, flavonoids are responsible for broad ranges of colors. Chrysanthemums are one of the most popular ornamental crops in the world, and there have been many efforts to change their flower color. In chrysanthemum flowers, cyanidin-based anthocyanin confers pink or red color, whereas terpenoid-based carotenoids are mainly responsible for yellow and green colors. However, blue colored chrysanthemums do not occur in nature. To date, there have been attempts to obtain blue or violet-colored chrysanthemum flowers through the introduction of a novel gene for accumulating delphinidin-based anthocyanins, while other studies have reported changing endogenous metabolites through the reconstruction of flavonoid biosynthesis. Since various transcription factors are involved in the regulation of flavonoid biosynthesis, it is important to understand not only the structural genes, but also the transcription factors required for the modification of flavonoid-based flower color. Therefore, in this paper, we describe the flavonoid biosynthetic pathway and its regulation, and review previous studies on the change in flower color through modification of flavonoid biosynthesis. This effort could be an important milestone in successfully achieving the modification of chrysanthemum flower color by means of plant biotechnology.

참고문헌 (82)

  1. Aharoni A, "The strawberry FaMYB1transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco", Plant J, 28, pp.319~332 (2001)
  2. Albert NW, "A conserved network of transcriptional activators and repressors regulates anthocyanin pigmentation in eudicots", Plant Cell, 26, pp.962~980 (2014)
  3. Andersen OM, "Flavonoids chemistry, biochemistry and applications", pp.464~522 (2006), CRC Press
  4. Anderson NO, "Flower breeding and genetics: Issues, challenges and opportunities for the 21st century", pp.389~437 (2006), Springer
  5. Azadi P, "Current status and biotechnological advances in genetic engineering of ornamental plants", Biotechnol Adv, 34, pp.1073~1090 (2016)
  6. Bedon F, "Subgroup 4 R2R3-MYBs in conifer trees: Gene family expansion and contribution to the isoprenoid- and flavonoid-oriented responses", J Exp Bot, 61, pp.3847~3864 (2010)
  7. Ben Zvi MM, "PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers", New Phytol, 195, pp.335~345 (2012)
  8. Berman J, "Red anthocyanins and yellow carotenoids form the color of orange-flower gentian (Gentiana lutea L. var. aurantiaca)", PLOS ONE, 11, pp.e0162410~ (2016)
  9. Bontpart T, "BAHD or SCPL acyltransferase? What a dilemma for acylation in the world of plant phenolic compounds", New Phytol, 208, pp.695~707 (2015)
  10. Brugliera F, "Isolation and characterization of a flavonoid 3'‐hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida", Plant J, 19, pp.441~451 (1999)
  11. Brugliera F, "Violet", blue chrysanthemums-metabolic engineering of the anthocyanin biosynthetic pathway results in novel petal colors, 2013, pp.54, 1696~1710
  12. Chen SM, "The identification of flavonoids and the expression of genes of anthocyanin biosynthesis in the chrysanthemum flowers", Biol Plant, 56, pp.458~464 (2012)
  13. Dahlbender B, "Nitrogen nutrition and the accumulation of free and sinapoyl‐bound malic acid in Raphanus sativus cotyledons", Planta, 161, pp.142~147 (1984)
  14. Davies KM, "Anthocyanins biosynthesis functions, and applications", pp.49~80 (2008), Springer
  15. Dowrick GJ, "The chromosomes of chrysanthemum, II: garden varieties", Heredity, 7, pp.59~72 (1953)
  16. Du H, "Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia", J Exp Bot, 66, pp.6563~6577 (2015)
  17. Dubos C, "MYBL2 is a new regulator of flavonoid biosynthesis in Arabidopsis thaliana", Plant J, 55, pp.940~953 (2008)
  18. Ford CM, "Cloning and characterization of Vitis vinifera UDP-Glucose: flavonoid 3-O-glucosyltransferase, a homologue of the enzyme encoded by the maize bronze-1 Locus that may primarily serve to glucosylate anthocyanidins in vivo", J Biol Chem, 273, pp.9224~9233 (1998)
  19. Gachon CM, "Plant secondary metabolism glycosyltransferases: the emerging functional analysis", Trends Plant Sci, 10, pp.542~549 (2005)
  20. Gou JY, "Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor", Plant Cell, 23, pp.1512~1522 (2011)
  21. Gross GG, "Synthesis of mono‐, di‐ and trigalloyl‐β‐D‐glucose by β‐glucogallin‐dependent galloyltransferases from oak leaves", Z Naturforsch, 38, pp.519~523 (1983)
  22. Grotewold E, "The genetics and biochemistry of floral pigments", Annu Rev Plant Biol, 57, pp.761~780 (2006)
  23. Hanks G, "A review of production statistics for the cut flower and foliage sector 2015 (Part of AHDB Horticulture funded project PO BOF 002a)", The National Cut Flower Centre, AHDB Horticulture, 102~ (2015)
  24. He H, "Flower colour modification of chrysanthemum by suppression of F3'H and overexpression of the exogenous Senecio cruentus F3'5'H gene", PLOS ONE, 8, pp.e74395~ (2013)
  25. Holton TA, "Cloning and expression of cytochrome P450 genes controlling flower colour", Nature, 366, pp.276~279 (1993)
  26. Hong Y, "Transcriptomic analyses reveal species-specific light-induced anthocyanin biosynthesis in chrysanthemum", BMC Genomics, 16, pp.202~ (2015)
  27. Hugueney P, "A novel cation-dependent O-methyltransferase involved in anthocyanin methylation in grapevine", Plant Physiol, 150, pp.2057~2070 (2009)
  28. Ibrahim RK, "Plant O-methyltransferases: molecular analysis, common signature and classification", Plant Mol Biol, 36, pp.1~10 (1998)
  29. Jadhav SKR, "Structural characterization of a flavonoid glycosyltransferase from Withania somnifera", Bioinformation, 8, pp.943~ (2012)
  30. Jones P, "UGT73C6 and UGT78D1, glycosyltransferases involved in flavonol glycoside biosynthesis in Arabidopsis thaliana", J Biol Chem, 278, pp.43910~43918 (2003)
  31. Kadomura-Ishikawa Y, "RNAi-mediated silencing and overexpression of the FaMYB1 gene and its effect on anthocyanin accumulation in strawberry fruit", Biol Plant, 59, pp.677~685 (2015)
  32. Katsumoto Y, "Engineering of the rose flavonoid biosynthetic pathway successfully generated blue-hued flowers accumulating delphinidin", Plant Cell Physiol, 48, pp.1589~1600 (2007)
  33. Kim DH, "Enhancing flower color through simultaneous expression of the B-peru and mPAP1 transcription factors under control of a flower-specific promoter", Int J Mol Sci, 19, pp.309~ (2018)
  34. Kim S, "High ambient temperature represses anthocyanin biosynthesis through degradation of HY5", Front Plant Sci, 8, pp.1787~ (2017)
  35. Koes R, "Flavonoids: A colorful model for the regulation and evolution of biochemical pathways", Trends Plant Sci, 10, pp.236~242 (2005)
  36. Kojima M, "Elucidation of the structure of a possible intermediate in chlorogenic acid biosynthesis in sweet potato root tissue", Plant Cell Physiol, 13, pp.1075~1084 (1972)
  37. Kondo T, "Structure determination of heavenly blue anthocyanin, a complex monomeric anthocyanin from the morning glory ipomea tricolor, by means of the negative NOE method", Tetrahedron Lett, 28, pp.2273~2276 (1987)
  38. Kroon J, "Cloning and structural analysis of the anthocyanin pigmentation locus Rt of Petunia hybrida: Characterization of insertion sequences in two mutant alleles", Plant J, 5, pp.69~80 (1994)
  39. Lam KC, "Structure, function, and evolution of plant O-methyltransferases", Genome, 50, pp.1001~1013 (2007)
  40. Lee YJ, "Cation dependent O-methyltransferases from rice", Planta, 227, pp.641~647 (2008)
  41. Lepiniec L, "Genetics and biochemistry of seed flavonoids", Annu Rev Plant Biol, 57, pp.405~430 (2006)
  42. Lim SH, "RNAi-mediated suppression of dihydroflavonol 4-reductase in tobacco allows fine-tuning of flower color and flux through the flavonoid biosynthetic pathway", Plant Physiol Biochem, 109, pp.482~490 (2016)
  43. Liu X, "Functional characterization of a flavonoid glycosyltransferase in sweet orange (Citrus sinensis)", Front Plant Sci, 9, pp.166~ (2018)
  44. Liu XF, "The identification of a MYB transcription factor controlling anthocyanin biosynthesis regulation in Chrysanthemum flowers", Sci Hortic, 194, pp.278~285 (2015)
  45. Luo J, "Convergent evolution in the BAHD family of acyl transferases: Identification and characterization of anthocyanin acyl transferases from Arabidopsis thaliana", Plant J, 50, pp.678~695 (2007)
  46. Maier A, "COP1", SPA ubiquitin ligase complexes repress anthocyanin accumulation under low light and high light conditions, 2015, pp.10, e970440~
  47. Maier A, "Light and the E3 ubiquitin ligase COP1", SPA control the protein stability of the MYB transcription factors PAP1and PAP2 involved in anthocyanin accumulation in Arabidopsis, 2013, pp.74, 638~651
  48. Matsui K, "AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis", Plant J, 55, pp.954~967 (2008)
  49. Mazza G, "Recent developments in the stabilization of anthocyanins in food products", Food Chem, 25, pp.207~225 (1987)
  50. Milkowski C, "Serine carboxypeptidase-like acyltransferases", Phytochemistry, 65, pp.517~524 (2004)
  51. "Status of ornamental crops" (2017)[URL]
  52. Mol J, "How genes paint flowers and seeds", Trends Plant Sci, 3, pp.212~217 (1998)
  53. Morita Y, "Japanese morning glory dusky mutants displaying reddishbrown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDPglucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, due to 4-bp insertions in the gene", Plant J, 42, pp.353~363 (2005)
  54. Mugford ST, "Serine carboxypeptidaselike acyltransferases from plants. Methods in Enzymology", Elsevier, 516, pp.279~297 (2012)
  55. Nakayama T, "Anthocyanin acyltransferases: Specificities, mechanism, phylogenetics, and applications", J Mol Catal B Enzymatic, 23, pp.117~132 (2003)
  56. Noda N, "Genetic engineering of novel bluer-colored chrysanthemums produced by accumulation of delphinidin-based anthocyanins", Plant Cell Physiol, 54, pp.1684~1695 (2013)
  57. Noda N, "Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism", Sci Adv, 3, pp.e1602785~ (2017)
  58. Noel JP, "Structural, functional, and evolutionary basis for methylation of plant small molecules. Recent Advances in Phytochemistry", Elsevier, 37, pp.37~58 (2003)
  59. Nozaki K, "Effects of high temperature on flower colour and anthocyanin content in pink flower genotypes of greenhouse chrysanthemum (Chrysanthemum morifolium Ramat.)", J Hortic Sci Biotechnol, 81, pp.728~734 (2006)
  60. Offen W, "Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification", EMBO J, 25, pp.1396~1405 (2006)
  61. Ogata J, "Plant biochemistry: Anthocyanin biosynthesis in roses", Nature, 435, pp.757~ (2005)
  62. Ohmiya A, "Molecular mechanisms underlying the diverse array of petal colors in chrysanthemum flowers", Breed Sci, 68, pp.119~127 (2018)
  63. Ohno S, "A bHLH transcription factor, DvIVS, is involved in regulation of anthocyanin synthesis in dahlia (Dahlia variabilis)", J Exp Bot, 62, pp.5105~5116 (2011)
  64. Ono E, "Yellow flowers generated by expression of the aurone biosynthetic pathway", Proc Natl Acad Sci USA, 103, pp.11075~11080 (2006)
  65. Park CH, "Anthocyanin and carotenoid contents in different cultivars of chrysanthemum (Dendranthema grandiflorum Ramat.) flower", Molecules, 20, pp.11090~11102 (2015)
  66. Polturak G, "Engineered gray mold resistance, antioxidant capacity, and pigmentation in betalain-producing crops and ornamentals", Proc Natl Acad Sci USA, 114, pp.9062~9067 (2017)
  67. Quattrocchio F, "The science of flavonoids", pp.97~122 (2006), Springer
  68. Schwarz-Sommer Z, "An everlasting pioneer: the story of Antirrhinum research", Nat Rev Genet, 4, pp.655~ (2003)
  69. St-Pierre B, "Origin and diversification of the BAHD superfamily of acyltransferases involved in secondary metabolism", Recent Adv Phytochem, 34, pp.285~315 (2000)
  70. Sung SY, "Comparative gene expression analysis in a highly anthocyanin pigmented mutant of colorless chrysanthemum", Mol Biol Rep, 40, pp.5177~5189 (2013)
  71. Tanaka Y, "Recent progress of flower colour modification by biotechnology", Int J Mol Sci, 10, pp.5350~5369 (2009)
  72. Tsuda S, "Flower color modification of Petunia hybrida commercial varieties by metabolic engineering", Plant Biotechnol, 21, pp.377~386 (2004)
  73. Ünligil UM, "Glycosyltransferase structure and mechanism", Curr Opin Struct Biol, 10, pp.510~517 (2000)
  74. Unno H, "Structural and mutational studies of anthocyanin malonyltransferases establish the features of BAHD enzyme catalysis", J Biol Chem, 282, pp.15812~15822 (2007)
  75. Wang X, "Structure, mechanism and engineering of plant natural product glycosyltransferases", FEBS Lett, 583, pp.3303~3309 (2009)
  76. Watanabe K, "CRISPR", Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil, 2017, pp.7, 10028~
  77. Xiang LL, "A novel bHLH transcription factor involved in regulating anthocyanin biosynthesis in Chrysanthemums (Chrysanthemum morifolium Ramat.)", PLOS ONE, 10, pp.e0143892~ (2015)
  78. Xu W, "Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes", Trends Plant Sci, 20, pp.176~185 (2015)
  79. Yamagishi M, "Two R2R3-MYB genes, homologs of petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of Asiatic hybrid lily", Plant Cell Physiol, 51, pp.463~474 (2010)
  80. Yamazaki M, "Two flavonoid glucosyltransferases from Petunia hybrida: molecular cloning, biochemical properties and developmentally regulated expression", Plant Mol Biol, 48, pp.401~411 (2002)
  81. Ye ZH, "An alternative methylation pathway in lignin biosynthesis in Zinnia", Plant Cell, 6, pp.1427~1439 (1994)
  82. Zhang Y, "Engineering anthocyanin biosynthesis in plants", Curr Opin Plant Biol, 19, pp.81~90 (2014)

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