본문 바로가기
KERD
논문KCI 등재

젖산균과 청국균으로 발효시킨 용안육의 항산화, 항암효과 및 일산화질소생성

Antioxidant, Anticancer Activities and Nitric Oxide Production of Euphoria longana Fermented with Lactic Acid Bacteria and Bacillus subtilis

남상해|손미예|이상원
한국식품저장유통학회 (The Korean Society of Food Preservation)|2007.10|Food Science and Preservation|제14권 제5호|pp. 531-537

1

조회

0

다운로드

9

인용

0

참고문헌

주제분류식품과학

초록

태국산과 베트남산의 용안육 원료 및 그 발효물의 전자공여능, 일산화질소(NO) 생성효과, 인체암 세포주인 자궁경부암세포(HeLa) 및 간암세포(HepG2)의 항암활성 효과를 조사하였다. 원료 용안육의 총 유기산은 태국산 용안육(473.49 mg/g)이 베트남산(148.48 mg/g) 용안육에 비하여 3.2배 높은 함량을 나타내었고, 전체 유기산의 각각 89.2%와 86.8%를 나타내었으며, 주요 유기산은 formic acid와 malic acid였다. 원료 용안육의 총 유리당은 베트남산(434.63 mg/g)이 태국산(378.77 mg/g)에 비하여 1.2배 높은 함량을 나타내었으며, 주요한 유리당은 sucrose, glucose와 fructose였다. 단핵세포 세포주인 RAW264.7 세포에서 발효 용안육의 NO 생성은 베트남산이 태국산에 비하여 약간 낮은 농도를 나타내었고, 원료 용안육과 비교하면 고농도보다 저농도 추출물 첨가에서 그 함량의 차이가 크게 나타났다. 원료 용안육의 추출물 600 μg에서 전자공여능은 베트남산이 41.72±3.59%로 태국산 30.20±4.80%에 비하여 1.4배 높았으며, 발효 용안육의 전자공여능은 B. subtilis로 발효시킨 베트남산 용안육의 추출물 600 μg에서 43.57±2.07%로 가장 높게 나타났다. 원료 용안육의 자궁경부암세포에 대한 항암활성은 베트남산이 태국산 용안육보다 대체로 비슷하였지만, 간암세포의 경우는 태국산 용안육 추출물 200 μg 농도에서 저해율이 46.13±4.80%로 베트남산 33.07±0.92%보다 높게 나타났다. 그리고 발효 용안육의 자궁경부암세포 및 간암세포에 대한 항암활성은 각각 B. subtilis로 발효시킨 베트남산 및 태국산 용안육 추출물 200 μg에서 저해율 39.21±1.46% 및 48.07±1.63%로 가장 높게 나타났다.감사의 글

Abstract

Antioxidant, anticancer activities and nitric oxide (NO) production of Vietnamese and Thai yonganyook (Euphoria longana) fermented with lactic acid bacteria and Bacillus subtilis were investigated. Total organic acid contents (TAC) of Thai raw yonganyook (473.49 mg/g, 89.2% of TAC) were higher 3.2 times than those of Vietnamese raw yonganyook (148.48 mg/g, 86.8% of TAC) and major organic acids of two materials were formic acid and malic acid. Total free sugars contents of Vietnamese raw yonganyook (434.63 mg/g) were higher 1.2 times than those of Thai raw yonganyook (378.77 mg/g) and major free sugar of two materials were sucrose, glucose and fructose. NO production of RAW264.7 cell treated with methanol extract of Vietnamese fermented yonganyook was shown to be a lower level than that of Thai fermented yonganyook. It's production by fermented yonganyook was strongly exhibited in low dose (0.2 mg/mL) than in high dose (1.0 mg/mL) as compared with raw yonganayook. Electron-donating ability (EDA) of Vietnamese raw yonganyook (41.72±3.59%) at 600 μg/assay was higher 1.4 times than that of Thai yonganyook (30.20±4.80%). EDA of Vietnamese yonganyook fermented with B. subtilis at 600 μg/assay was 43.57±2.07%, which was the highest level of all samples tested. Anticancer activity of raw yonganyook on human HeLa cell was similar to between Vietnamese and Thai yonganyook. In inhibitory effect of HepG2 cell growth, methanol extract of Thai yonganyook (46.13±4.80%) was higher than that of Vietnamese yonganyook (33.07±0.92%). Inhibitory activity of fermented yonganyook on HeLa and HepG2 cell growth were 39.21±1.46% and 48.07±1.63%, when 200 μg/assay of methanol extract of Vietnamese and Thai yonganyook fermented with B. subtilis were used, respectively.

참고문헌 (32)

  1. "Quality evaluation and components of Euphoria longana", 33, pp.191~193 (2002)
  2. "a major fatty acid component of Euphoria longana seed oil", pp.317~320 (1968)
  3. "Hydrolysis products of the major fatty acids from Euphoria longana seed oil", pp.525~529 (1970)
  4. "Adenosine, the anxiolytic-like principle of the arillus of Euphoria longana", 65, pp.115~119 (1999)
  5. "Development of biologically active compounds from edible plant sources-VIII. Isolation of platelet aggregation inhibitory compounds from the arillus of Euphoria longana L", 47, pp.130~134 (2004)
  6. "Influence of acetonylgeraniin a hydrolyzable tannin from Euphoria longana on orthostatic hypertension in a rat model", pp.297~300 (1994)
  7. "Antihypertensive effect of corilagin in the rat", 73, pp.1425~1429 (1995)
  8. "Chemical investigation on the leaves of Euphoria longana", pp.2847~2848 (1971)
  9. "5-hydroxymethyl- 2-furfuraldehyde, anticonvulsant furan from the arillus of Euphoria longana L", 48, pp.32~34 (2005)
  10. "Effects of the Longanae Arillus on the norepinephrine contents of immobilzation stressed mice brain" (2002)
  11. "Effects of the Euphoria longana L and Hoelen cum Pini Radix on the serotonin contents of immobilzation stressed mice brain" (2002)
  12. "Studies on the processing of crude drugs on the constituents and biological activities of Glycyrrhizae radix by processing", 27, pp.196~206 (1996)
  13. "Fermentation of antler and its biological activity", pp.233~237 (1994)
  14. "Preparation and quality characteristics of the fermentation product of ginseng by lactic acid bacteria(FGL)", 30, pp.88~94 (2006)
  15. "Comparison of fermentability and characteristics of fermented broth for white ginseng, red ginseng and extruded white ginseng", 11, pp.119~126 (2007)
  16. "Effects of fermented wild-ginseng culture by-products on growth performance, blood characteristics, meat quality and ginsenoside concentration of meat in finishing pigs", 49, pp.329~340 (2007)
  17. "Antidiabetic and oxidative stress relieving effects of fermented red ginseng" (2007)
  18. "Enhancement of immune activities of Kadsura japonica Dunal through conventional fermentation process", 15, pp.162~169 (2007)
  19. "Major chemical components in fermented beverages of Liriopis tuber", 10, pp.281~287 (2000)
  20. "Changes of volatile organic compounds of Rhus verniciflua S. Bark by fermentation", 14, pp.308~314 (2007)
  21. "Effect of wogonin, a plant flavone from Scutellafia radix, on the suppression of cyclooxygenase-2 and the induction of inducile nitric oxide synthase in lipopolysaccharide- treated RAW264.7 cell", 61, pp.1195~1203 (2001)
  22. "Flavonoid content of several vegetables and their antioxidant mushrooms(Agaricus bisporus)", 80, pp.561~566 (2000)
  23. "Reexamination and further development of a precise and rapid dye method for measuring cell growth", cell kill, 1989, pp.immu(l), 203~210
  24. "Changes in free sugars, organic acid, free amino acids and minerals by harvest time and parts of Acanthopanax koreanum", 43, pp.106~109 (2000)
  25. "Role of NO in activation of NFκB by PM2 5 in lung epithelial cells", 52, pp.616~626 (2002)
  26. "Modulatory effects of 21 kinds of medicinal herbs including herba Pogostemi(Agastache rugosa) on nitric oxide production in macrophage cell line RAW264.7 cells", 48, pp.411~417 (2005)
  27. "Induction of nitric oxide and TNF-α by herbal plant extracts in mouse macrophages", 29, pp.342~348 (2000)
  28. "Effect of several herb drugs Hep G2 cells and mouse peritoneal macrophage", 12, pp.95~111 (1997)
  29. "Screening for antioxidative activity of oriental medicinal plant materials", 10, pp.171~176 (2002)
  30. "Screening for antioxidant activity of plant medicinal extracts", 47, pp.135~140 (2004)
  31. "Screening of natural antioxidant from plant and their antioxidative effect", pp.142~148 (1992)
  32. "Screening of the antioxidant activity of some medicinal plants", 36, pp.333~338 (2004)

관련 논문AI추천

발효콩 대두황권의 항산화 및 항암효과남상해, 손미예, 이상원한국식품저장유통학회 · Food Science and Preservation2007 · 제14권 제5호무처리 및 증숙처리 산수유 추출물의 항산화 및 생리활성구율리, 홍주헌한국식품저장유통학회 · Food Science and Preservation2018 · 제25권 제3호꾸지뽕나무 열매 당침액 이용 콤부차의 이화학적 품질 특성 및 인체 폐암세포 항증식 활성박종철, 송경주, 이진욱, 이창무, 조미애, 최호칠한국식품저장유통학회 · Food Science and Preservation2024 · 제31권 제4호상황버섯, 차가버섯, 느타리버섯 발효식초의 항산화와 항암활성 효과김홍식, 서훈석, 우선희, 정봉환, 조용구한국약용작물학회 · 한국약용작물학회지2010 · 제18권 제2호인체 유래 암세포주에 대한 Vitelotte 추출물의 항암활성이재혁대한산업경영학회 · 산업융합연구2025 · 제23권 제12호보리수 열매 식초 발효 중 이화학적 특성, phytochemical 함량 및 생리활성 변화조계만, 주옥수, 황정은한국식품저장유통학회 · Food Science and Preservation2017 · 제24권 제1호

이 간행물의 다른 논문 (5건)

홍삼 첨가 액상청국장의 기능성에 대한 마이크로웨이브 최적 추출조건 예측이보미, 도정룡, 김현구pp. 474-480감마선 조사와 실크 펩타이드 병용처리가 세균의 방사선 감수성 및 햄버거 패티의 저장 안정성에 미치는 영향변명우, 김재훈, 박진규, 이주운, 김왕근, 송범석, 황영정pp. 481-486Physicochemical Properties of Rice Extrudate Added with Onion Kimchi Powder강성구, 박양균, Ittiporn Keawpengpp. 504-510과메기 첨가 김치의 숙성과 품질특성오승희, 김순동, 정유경pp. 526-530발효콩 대두황권의 항산화 및 항암효과남상해, 손미예, 이상원pp. 538-544
원문 보기