스마트폰 카메라를 활용한 뇨시험지 당뇨병관련 융합 분석인자의 색기반 분석에 미치는 외부 조도 영향
Effect of Illuminance on Color-based Analysis of Diabetes-Related Urine Fusion Analytes on Dipstick Using a Smartphone Camera
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초록
최근 현장검사장치의 소형화 및 디지털화가 급격히 빠르게 진화하고 있다. 뇨검사는 일반인이 요시험지인 딥스틱을 이용하여 시각적인 색비교를 통해 자가진단이 가능하기에 색인지 기술에 대한 연구가 많이 이루어지고 있다. 본 연구는 요시험지검사에 사용되는 딥스틱(Dipstick Pad)의 색 변화를 스마트폰 카메라를 이용하여 얻어진 이미지로부터 RGB 값을 분석하였다. 비교 대상은 가장 많이 검사하는 질환으로 당뇨 증상과 관련 있는 요소인 당, 케톤체, pH의 양적변화에 대한 뇨 색 변화를 관찰하였다. 본 실험에서 일반적인 조도의 영향을 기준으로 밝은 조도와 어두운 조도 조건에서 뇨시험지상의 색변화에서 추출된 RGB값의 변화를 관찰하였다. 결과적으로 밝은 조도 조건에서의 색상 추출값이 높고 농도에 따른 반영이 잘 이루어지는 반면 낮은 조도 영역에는 색상 추출값이 낮게 나타났다. 따라서, 색 분리 알고리즘의 문제점을 개선하고자 RGB 수치의 변화값을 제시하였다.
Abstract
Recently, the miniaturization and digitalization for the inspection devices of point-of-care testing (POCT) are rapidly evolving. In the urine test, a lot of researches on index paper technology are being conducted because people can be self-diagnosed through visual color comparison using a urine test paper, Dipsick. The purpose of this study is to analyze the RGB values from the color changes on Dipstick Pad, which isused for urine test,using a smartphone camera. To this end, the primary ,analytes in urine wasdiabetes-related parameters such as glucose, ketone body and pH, which is the most frequently tested elements, and we pursuited to quantify the changes in dipstick color caused from artificial urine containing different ranges of sugar, ketone body, and pH. In this experiment, changes in RGB values under bright and dark illuminances were compared, and changes in RGB value were monitored as a function of concentration of analytes under the ambient illumination of laboratory. As a result, color separation at the bright luminance region was good, but it did not appearat the low luminance region, and the changed profiles in RGB value under different illuminances was suggested to correct the problem of the color separation algorithm.
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