LC-MS/MS를 이용한 단백질 의약품 맵핑 교수법
Educational Peptide Mapping of Protein-based Biopharmaceuticals by using LC-MS/MS
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초록
본 실험은 효용성이 넓어지고 있는 바이오의약품 시장에서 질량분석기를 이용한 정밀 분석법을 제시한다. 단백질 의약품인인성장호르몬(Somatotopin)을 분석하는 다양한 기술 중, 생화학적 시료 전처리를 통해 펩티드화 시킨 단백질을 LC-MS/MS 분석법으로 분석하였다. 분석과정은 액체크로마토그래피를 이용한 분리분석과 질량분석을 이용한 MS 및 MS/MS 분석으로 수행하였다. 인성장호르몬의 경우 21개의 트립틱 펩티드로 절단할 수 있는데, 본 실험을 통해 이들 중 13개의 펩티드가 평균 1 ppm에러 이내로 이론적 예측치와 일치하였다.
Abstract
This experiment presents a precise analysis method using a mass spectrometer in the biopharmaceutical market, where utilityis expanding. Among various techniques for analyzing the protein drug, somatotropin, the peptide fragments through biochemicalsample preparation was analyzed by LC-MS/MS characterization. The analysis process was performed by separation analysis usingnanoUPLC and MS/MS analysis using Orbitrap. In the case of somatotropin with 21 tryptic peptides, 13 of them were consistentwith theoretical predictions within an average of 1 ppm error.
참고문헌 (6)
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