Lipopolysaccharide에 의해 유도된 RAW 264.7 cell에서의 견우자 (Pharbitis nil Choisy) 추출물의 염증억제 효과
Anti-Inflammatory Effects of Pharbitis nil Choisy in Lipopolysaccharide-Induceds RAW 264.7 Cells
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초록
This study was investigated to find a candidate which has an anti-inflammatory activity on RAW 264.7 cells. It was performed by NO assay, RT-PCR and western blot analysis. In the course of screening, it was found that extracts of Pharbitisnil Choisy showed strong activity of anti-inflammation. Therefore, extracts of Pharbitis nil Choisy was sequentially fractionated with hexane, m ethylene chloride, ethyl-acetate, butanol and water. Of the sequential solvent fractions, the ethylacetate fraction inhibited the mRNA and protein expression of LPS-induced iNOS and COX-2. Taken together, these results suggest that extracts of Pharbitis nil Choisy have significant effects on inflammatory factors, and may be a potential anti-inflammatory therapeutic plant.
Abstract
This study was investigated to find a candidate which has an anti-inflammatory activity on RAW 264.7 cells. It was performed by NO assay, RT-PCR and western blot analysis. In the course of screening, it was found that extracts of Pharbitisnil Choisy showed strong activity of anti-inflammation. Therefore, extracts of Pharbitis nil Choisy was sequentially fractionated with hexane, m ethylene chloride, ethyl-acetate, butanol and water. Of the sequential solvent fractions, the ethylacetate fraction inhibited the mRNA and protein expression of LPS-induced iNOS and COX-2. Taken together, these results suggest that extracts of Pharbitis nil Choisy have significant effects on inflammatory factors, and may be a potential anti-inflammatory therapeutic plant.
참고문헌 (19)
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