인동등 에탄올 추출물의 HaCaT세포에서 Hyaluronic acid 생성 증가 효과
Effect of Lonicera japonica Ethanol Extract on Hyaluronic Acid Production in HaCaT Cells
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초록
본 연구에서는 인동등의 에탄올 추출물이 HA 생성에 미치는 영향을 확인하고자 하였다. 인동등 에탄올 추출물 50, 100 및 200μg/mL 처리에서 각각 vehicle control 대비 39.26%, 50.14%, 60.01%의 HA 생성 증가를 보였다. HA량은 HA를 생성하는 HAS2와 분해하는 hyaluronidase 활성에 영향을 받기 때문에 인동등 에탄올 추출물이 hyaluronidase 활성을 저해하는지를 실험한 결과 인동 에탄올 추출물은 hyaluronidase 활성을 저해 효능을 보이지 않았다. HAS2 유전자 발현에 미치는 영향을 실험해 본 결과 50, 100, 200μg/mL 처리에서 vehicle control 대비 각각 1.99, 6.23, 7.36배의 증가를 보였다. 이를 통해 인동등 에탄올 추출물이 HAS2의 전사과정에 관여하여 유전자 발현을 유도함으로서 HA량을 늘릴 수 있음을 확인할 수 있었다. 따라서 인동 에탄올 추출물이 피부에서 보습, 항노화, 항주름 효과를 나타낼 수 있을 것으로 생각되며 기능성 화장품 원료로 개발이 가능할 것으로 보인다.
Abstract
Hyaluronic acid(HA) is composed of repeating disaccharide(D-glucuronic acid and N-acetyl-D-glucosamine). It has been known to play important roles in physiological and pathological events such as cell immigration and inflammation. Especially, HA is deeply associated with structural stiffness and hydration in skin. Production of HA by hyaluronic acid synthases(HASs), especially HAS2 in human and degradation of that by hyaluronidase are responsible for the HA homeostasis. Destruction of balance between production and degradation of HA could result in skin aging. Therefore, researches on molecules which induce the HAS up-regulation or inhibit the hyaluronidase activity have been carried out. In this study, we investigated the effect of Lonicera japonica ethanol extract(LJ) on HA production in HaCaT cells. LJ increased the 60% HA production compared to that of vehicle control, which could be mediated by HAS2 mRNA up-regulation not by hyaluronidase inhibition. Taken together, LJ could be developed for anti-aging and anti-wrinkle cosmetics. It still remains to be elucidated which compounds are responsible for HAS2 mRNA up-regulation and which signal pathways are involved in transcriptional regulation.
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