해양 홍조류 에탄올 추출물의 항균 및 항산화 활성
Antimicrobial and Antioxidant Activities of Ethanol Extracts from Marine Red Algae
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The aims of the current study were to analyze the bioactive substances of ethanol extracts from domestic marine red algae strains G. verrucosa, G. vermiculophylla, C. tenellus, and G. furcata, and to evaluate their antioxidant and antibacterial activities. Analysis indicated that the contents of total polyphenols and flavonoids were higher than other red algae extracts, with total polyphenols and flavonoids of 8.08±1.04 mg/g and 66.94±6.12 mg/g, respectively. Results of tests of SOD-like activity in the four species of red algae found that C. tenellus had the highest activity at 121.76±1.92 unit/mg. Similar to SOD-like activity, ascorbate peroxidase (APX) activity was the highest at 6.13±0.68 units/mg in stone agar, as a result of H2O2 scavenging. The catalase (CAT) activity of G. furcata was the highest among the four red algae extracts at 0.66±0.11 unit/mg. All four red algae extracts showed DPPH radical scavenging activities in a concentration-dependent manner. At 100 mg/mL, G. furcata had the highest activity at 9.05±4.20% followed by G. vermiculophylla at 8.09±6.16%. In addition, the antimicrobial activity of the ethanol extracts of all four types of red algae indicated minimum inhibitory concentration (MIC) of 0.4 to 6.25 mg/mL for the experimental strains. In the case of S. epidermidis, G. verrucosa had the greatest antibacterial activity in gram-positive bacteria at 0.625 mg/mL. Ethanol extracts of all four types of red algae demonstrated stronger antibacterial activity in gram negative bacteria. The MIC showed the strongest antimicrobial spectrum in the extract of G. verrucosa. Minimum bacterial concentration (MBC) for the ethanol extracts of four types of red algae was found to be in the range of 0.4 to 6.25 mg/mL, with the strongest activity in G. verrucosa and G. vermiculophylla at 1.25 ~ 5 and 0.8 ~ 6.25 mg/mL, respectively. Taken together, these results indicate that ethanol extracts of domestic marine red algae G. verrucosa, G. vermiculophylla, C. tenellus, and G. furcata have high antimicrobial and antioxidant activities, and should be considered for use as a functional cosmetic compound.
Abstract
The aims of the current study were to analyze the bioactive substances of ethanol extracts from domestic marine red algae strains G. verrucosa, G. vermiculophylla, C. tenellus, and G. furcata, and to evaluate their antioxidant and antibacterial activities. Analysis indicated that the contents of total polyphenols and flavonoids were higher than other red algae extracts, with total polyphenols and flavonoids of 8.08±1.04 mg/g and 66.94±6.12 mg/g, respectively. Results of tests of SOD-like activity in the four species of red algae found that C. tenellus had the highest activity at 121.76±1.92 unit/mg. Similar to SOD-like activity, ascorbate peroxidase (APX) activity was the highest at 6.13±0.68 units/mg in stone agar, as a result of H2O2 scavenging. The catalase (CAT) activity of G. furcata was the highest among the four red algae extracts at 0.66±0.11 unit/mg. All four red algae extracts showed DPPH radical scavenging activities in a concentration-dependent manner. At 100 mg/mL, G. furcata had the highest activity at 9.05±4.20% followed by G. vermiculophylla at 8.09±6.16%. In addition, the antimicrobial activity of the ethanol extracts of all four types of red algae indicated minimum inhibitory concentration (MIC) of 0.4 to 6.25 mg/mL for the experimental strains. In the case of S. epidermidis, G. verrucosa had the greatest antibacterial activity in gram-positive bacteria at 0.625 mg/mL. Ethanol extracts of all four types of red algae demonstrated stronger antibacterial activity in gram negative bacteria. The MIC showed the strongest antimicrobial spectrum in the extract of G. verrucosa. Minimum bacterial concentration (MBC) for the ethanol extracts of four types of red algae was found to be in the range of 0.4 to 6.25 mg/mL, with the strongest activity in G. verrucosa and G. vermiculophylla at 1.25 ~ 5 and 0.8 ~ 6.25 mg/mL, respectively. Taken together, these results indicate that ethanol extracts of domestic marine red algae G. verrucosa, G. vermiculophylla, C. tenellus, and G. furcata have high antimicrobial and antioxidant activities, and should be considered for use as a functional cosmetic compound.
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