육미지황탕 추출물 함유 화장품이 SKH-1 생쥐의 광노화 피부와 주름개선 효과
Photoaging Skin and Wrinkle Improvement Effects of Cosmetics Containing Yukmijiwhangtang Extract in SKH-1 Hairless Mice
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In this study, a natural cosmetic product containing 1% extract of Yukmijiwhangtang was prepared and applied to SKH-1 hairless mice repeatedly irradiated with UVB. The following results obtained experimentally verify its potential as a cosmetic material with photoaging skin inhibition and wrinkle improvement effects. 1. The change in skin moisture value was 33.9±3.2% in the YM group, which was significantly (p<0.001) higher than the control group , which was 16.6±2.6%. 2. The dorsal skin wrinkle value was 0.020±0.008mm in the YM group, which significantly (p<0.05) decreased compared to the control group, which was 0.072±0.003mm. 3. Optical findings of skin wrinkles showed that fine wrinkles decreased in the YM group compared to the control group. Furthermore, deep and bold wrinkles were much less and slightly observed in the YM group compared to the control group . 4. The change in dead skin cell value of the skin was lower in YM group at 19.9±3.8 µ2m compared to the control group at 24.0±0.0 µ2m. 5. Epidermal epithelial cell proliferation of the skin decreased in the YM group compared to both control group and positive control group. The epithelial cell layer of the epidermis decreased to 5-6 layers, resulting in the decrease in skin thickness. 6. Changes in the stainability of collagen fibers in the dermis layer of the skin were slightly impaired in the papillary layer of the dermis in the YM group compared to both the control and positive control groups, and the stainability to eosin or aniline blue was stronger. Based on the results, it can be concluded that the natural cosmetics applied with essence + cream containing 1% of Yukmijiwhangtang extract was effective in improving and preventing UVB-damaged photoaging skin and wrinkles.
Abstract
In this study, a natural cosmetic product containing 1% extract of Yukmijiwhangtang was prepared and applied to SKH-1 hairless mice repeatedly irradiated with UVB. The following results obtained experimentally verify its potential as a cosmetic material with photoaging skin inhibition and wrinkle improvement effects. 1. The change in skin moisture value was 33.9±3.2% in the YM group, which was significantly (p<0.001) higher than the control group , which was 16.6±2.6%. 2. The dorsal skin wrinkle value was 0.020±0.008mm in the YM group, which significantly (p<0.05) decreased compared to the control group, which was 0.072±0.003mm. 3. Optical findings of skin wrinkles showed that fine wrinkles decreased in the YM group compared to the control group. Furthermore, deep and bold wrinkles were much less and slightly observed in the YM group compared to the control group . 4. The change in dead skin cell value of the skin was lower in YM group at 19.9±3.8 µ2m compared to the control group at 24.0±0.0 µ2m. 5. Epidermal epithelial cell proliferation of the skin decreased in the YM group compared to both control group and positive control group. The epithelial cell layer of the epidermis decreased to 5-6 layers, resulting in the decrease in skin thickness. 6. Changes in the stainability of collagen fibers in the dermis layer of the skin were slightly impaired in the papillary layer of the dermis in the YM group compared to both the control and positive control groups, and the stainability to eosin or aniline blue was stronger. Based on the results, it can be concluded that the natural cosmetics applied with essence + cream containing 1% of Yukmijiwhangtang extract was effective in improving and preventing UVB-damaged photoaging skin and wrinkles.
참고문헌 (28)
- Berneburg M, "Photoaging of human skin", Photodermatol Photoimmunol Photomed, 16, pp.239~244 (2000)
- Chiba K, "Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin", Skin Pharmacol Physiol, 16, pp.242~251 (2003)
- Chung JH, "Modulation of skin collagen metabolism in aged and photoaged human skin in vivo", J Invest Dermatol, 117, pp.1218~1224 (2001)
- Chowjarean V, "A natural skin anti-ageing serum containing pseudobulb ethanolic extract of Grammatophyllum speciosum: a randomized double-blind, placebo-controlled trial", Int J Cosmet Sci, 41, pp.548~557 (2019)
- Gilchrest BA, "A review of skin ageing and its medical therapy", Br J Dermatol, 135, pp.867~875 (1996)
- Guyton KZ, "Induction of the mammalian stress response gene GADD153 by oxidative stress: role of AP-1 element", Biochem J, 314, pp.547~554 (1996)
- Hsu SM, "Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures", J Histochem Cytochem, 29, pp.577~580 (1981)
- Jeon HS, "Histological studies on the inhibitory effects of Yukmijiwhangtang on photoaging skin induced by UVB irradiation in SKH-1 mice", Kor J Aesthet Cosmetol, 11, pp.239~252 (2013)
- Jesumani V, "Potential use of seaweed bioactive compounds in skincare : a review", Mar Drugs, 17, pp.688~ (2019)[DOI]
- Kim ES, "Anti-oxidant function of genistein against H2O2-induced oxidative stress in HaCaT keratinocytes", Kor J Aesthet Cosmetol, 10, pp.541~547 (2012)
- Kim YC, "Chang BS : Inhibitory effects of nude pack containing black tea water extract on skin wrinkle formation in hairless mice", Appl Microsc, 41, pp.129~137 (2011)
- Kiss I, "The effect of high and low ultraviolet-B dose exposure on the degree of hairless mouse skin wrinkling", Photochem Photobiol, 53, pp.109~112 (1991)
- Koo HJ, "The suppressive effect of Pueraria lobata root extract and its biotransformed preparation against skin wrinkle formation", Korean J Plant Res, 30, pp.272~279 (2017)
- Kwon MH, "Inhibitory effects of Peonia japonica water extract on skin aging (I) : Focussed on alleviative effects of inflammation and skin barrier damage", J Environ Toxicol, 24, pp.159~167 (2009)
- Lee JK, "Analysis of studies on Yukmijihwang-tang for establishment of evidence based medicine", Kor J Orient Physiol Pathol, 23, pp.15~26 (2009)
- Lu YP, "Time course for early adaptive responses to ultraviolet B light in the epidermis of SKH-1 mice", Cancer Res, 59, pp.4591~4602 (1999)
- Masak H, "Detection of hydroxygen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation", Biochem Biophys Res Commun, 206, pp.474~479 (1995)
- Meinhard W, "Solar UV irradiation and dermal photoaging", J Photochem Photobiol B, 63, pp.41~51 (2001)
- Nguyen JK, "Bioactive ingredients in Korean cosmeceuticals: Trends and research evidence", J Cosmet Dermatol, 19, pp.1555~1569 (2020)[DOI]
- Oberyszyn TM, "Inhibitory effects of pentoxifylline on ultraviolet B light-induced cutaneous inflammation", Mol Carcinog, 22, pp.216~258 (1998)
- Park KJ, "Anti-wrinkle activity of Lindera obtusiloba extract", J Soc Cosmet Scientists Korea, 35, pp.317~323 (2009)
- Pinkus R, "Role of oxidants and antioxidants in the induction of AP-1, NF-κB, and glutathione S-transferase gene expression", J Biol Chem, 271, pp.13422~13429 (1996)
- Sander CS, "Photoaging is associated with protein oxidation in human skin in vivo", J Invest Dermatol, 118, pp.618~625 (2002)
- Sharmeen JB, "Essential oils as natural sources of fragrance compounds for cosmetics and cosmeceuticals", Molecules, 26, pp.666~ (2021)[DOI]
- Shin JY, "Explanation of Bangyakhappyun", pp.44~ (1984), Sungbosa
- Sundaresan M, "Requirement for generation of H2O2 for platelet-derived growth factor signal transduction", Science, 270, pp.296~299 (1995)
- Uitto J, "Dermatology in general medicine, Vol. 1", pp.299~314 (1993), McGraw-Hill
- Varani J, "Inhibition of type 1 procollagen synthesis by damaged collagen in photoaged skin and by collagenase-degraded collagen in vitro", Am J Pathol, 158, pp.931~942 (2001)