한국인에 있어서 혈소판 풍부 피브린에 함유된 성장인자의 확인
Confirmation of growth factors contained in platelet-rich fibrin in Koreans
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Various biomaterials are used in dental surgeries, including oral maxillofacial, periodontal, and implant surgeries. There is currently much research on synthetic bone grafts. Since synthetic bone lacks an osteo-induction effect despite having osteo-conduction, efforts have been made to increase the rate and quantity of bone formation as well as accelerate the rate of regeneration of damaged tissues by using a mixture of synthetic bone and materials having bone-inducing effects, such as growth factors and bone-formation proteins. The representative material among these is platelet-rich fibrin, which contains osteo-inducing growth factors from autogenous blood. Ten males in their 20s who do not smoke and are healthy without any systemic diseases were selected for the extraction of 20 mL of blood. After centrifuging blood, platelet-rich fibrin was obtained. Exudates were extracted by putting the blood in a bowl for 10 minutes. After preserving the extracted exudates at -70oC, enzyme-linked immunosorbent assay analysis was performed for quantitative evaluation of three growth factors, platelet-derived growth factor BB, insulin-like growth factor-1, and vascular endothelial growth factor. In this study, basic evidence about the clinical use of platelet-rich fibrin in various dental surgeries was obtained, and the role of platelet-rich fibrin was also elucidated by examining the roles of the three growth factors.
Abstract
Various biomaterials are used in dental surgeries, including oral maxillofacial, periodontal, and implant surgeries. There is currently much research on synthetic bone grafts. Since synthetic bone lacks an osteo-induction effect despite having osteo-conduction, efforts have been made to increase the rate and quantity of bone formation as well as accelerate the rate of regeneration of damaged tissues by using a mixture of synthetic bone and materials having bone-inducing effects, such as growth factors and bone-formation proteins. The representative material among these is platelet-rich fibrin, which contains osteo-inducing growth factors from autogenous blood. Ten males in their 20s who do not smoke and are healthy without any systemic diseases were selected for the extraction of 20 mL of blood. After centrifuging blood, platelet-rich fibrin was obtained. Exudates were extracted by putting the blood in a bowl for 10 minutes. After preserving the extracted exudates at -70oC, enzyme-linked immunosorbent assay analysis was performed for quantitative evaluation of three growth factors, platelet-derived growth factor BB, insulin-like growth factor-1, and vascular endothelial growth factor. In this study, basic evidence about the clinical use of platelet-rich fibrin in various dental surgeries was obtained, and the role of platelet-rich fibrin was also elucidated by examining the roles of the three growth factors.
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