Induction apoptosis and G2/M phase cell cycle arrest by non-thermal plasma in human osteosarcoma MG-63 cell line
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초록
Osteosarcoma is a malignant bone tumor that develops in the osteoblast cells that form the outer covering of bone. It is the most frequent malignant bone tumor found at 10-14 years and at >65 years of age. Atmospheric pressure plasma has advantages of high density and rich chemical agents without elevation of the substrate temperature. These non-equilibrium characteristics show promising applications in the biomedical field, opening a new research area called “Plasma Medicine”, which includes sterilization, coagulation, wound healing, and cancer treatment. However, the mechanism of apoptosis caused by plasma treatment in osteosarcoma is not well understood. In this study, the researchers show that microwave plasma causes selective cell cycle arrest and apoptosis in osteosarcoma cells in vitro. An inhabitation of cell growth of microwave plasma showed that this plasma has selective cytotoxic effects on MG-63 cells in comparison to hFOB 1.19 cells. Also, the resesearchers observed that microwave plasma treatmenst significantly showed disruption and aggregation of F-actin. The results demonstrated that treatment of MG-63 cells with microwave plasma induced cell cycle arrest in the G2/M phase, using flow cytometry and western blot assay. To detect PARP and DFF-45 cleavage or a decrease as a result of caspase-3 activation, MG- 63 cells were treated either with various times of microwave plasma. The research studies demonstrated that microwave plasma induced G2/M cell phase arrest and triggered apoptosis.
Abstract
Osteosarcoma is a malignant bone tumor that develops in the osteoblast cells that form the outer covering of bone. It is the most frequent malignant bone tumor found at 10-14 years and at >65 years of age. Atmospheric pressure plasma has advantages of high density and rich chemical agents without elevation of the substrate temperature. These non-equilibrium characteristics show promising applications in the biomedical field, opening a new research area called “Plasma Medicine”, which includes sterilization, coagulation, wound healing, and cancer treatment. However, the mechanism of apoptosis caused by plasma treatment in osteosarcoma is not well understood. In this study, the researchers show that microwave plasma causes selective cell cycle arrest and apoptosis in osteosarcoma cells in vitro. An inhabitation of cell growth of microwave plasma showed that this plasma has selective cytotoxic effects on MG-63 cells in comparison to hFOB 1.19 cells. Also, the resesearchers observed that microwave plasma treatmenst significantly showed disruption and aggregation of F-actin. The results demonstrated that treatment of MG-63 cells with microwave plasma induced cell cycle arrest in the G2/M phase, using flow cytometry and western blot assay. To detect PARP and DFF-45 cleavage or a decrease as a result of caspase-3 activation, MG- 63 cells were treated either with various times of microwave plasma. The research studies demonstrated that microwave plasma induced G2/M cell phase arrest and triggered apoptosis.
참고문헌 (27)
- Ritter J, "Osteosarcoma", Ann Oncol, 21, pp.vii320~vii325 (2010)[DOI]
- F Eilber, "Adjuvant chemotherapy for osteosarcoma: a randomized prospective trial.", Journal of Clinical Oncology, 5(1), pp.21~26 (1987), American Society of Clinical Oncology (ASCO)[DOI]
- Hogendoorn PC, "Bone sarcomas: ESMO clinical practice guidelines for diagnosis, treatment and follow-up", Ann Oncol, 21, pp.v204~v213 (2010)[DOI]
- Masayuki Morishita, "AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway", International Journal of Oncology, 50(1), pp.23~30 (2017), Spandidos Publications[DOI]
- Suzuki K, "Proliferation-related activity in endothelial cells is enhanced by micropower plasma", Biomed Res Int, 2016, pp.4651265~ (2016)[DOI]
- Y. Liu, "Nonthermal Atmospheric Plasmas in Dental Restoration", Journal of Dental Research, 95(5), pp.496~505 (2016), SAGE Publications[DOI]
- Karki SB, "Miniature dielectric barrier discharge nonthermal plasma induces apoptosis in lung cancer cells and inhibits cell migration", Biomed Res Int, 2017, pp.8058307~ (2017)[DOI]
- 이세영, "Nonthermal Plasma Induces Apoptosis in ATC Cells: Involvement of JNK and p38 MAPK-Dependent ROS", Yonsei Medical Journal, 55(6), pp.1640~1647 (2014), 연세대학교의과대학[DOI]
- Yonghao Ma, "Non-Thermal Atmospheric Pressure Plasma Preferentially Induces Apoptosis in p53-Mutated Cancer Cells by Activating ROS Stress-Response Pathways", PLoS ONE, 9(4), pp.e91947~ (2014), Public Library of Science (PLoS)[DOI]
- Ansar Karimian, "Multiple functions of p21 in cell cycle, apoptosis and transcriptional regulation after DNA damage", DNA Repair, 42, pp.63~71 (2016), Elsevier BV[DOI]
- X. Wang, "p53: Protection against Tumor Growth beyond Effects on Cell Cycle and Apoptosis", Cancer Research, 75(23), pp.5001~5007 (2015), American Association for Cancer Research (AACR)[DOI]
- SOHEI KONDO, "Apoptosis by Antitumor Agents and Other Factors in Relation to Cell Cycle Checkpoints.", Journal of Radiation Research, 36(1), pp.56~62 (1995), Oxford University Press (OUP)[DOI]
- Hickman JA, "Apoptosis induced by anticancer drugs", Cancer Metastasis Rev, 11, pp.121~139 (1992)
- K Boland, "Paracrine control of tissue regeneration and cell proliferation by Caspase-3", Cell Death & Disease, 4(7), pp.e725~e725 (2013), Springer Nature[DOI]
- Akao Y, "Arsenic-induced apoptosis in malignant cells in vitro", Leuk Lymphoma, 37, pp.53~63 (2000)
- Xu Yan, "Plasma-Induced Death of HepG2 Cancer Cells: Intracellular Effects of Reactive Species", Plasma Processes and Polymers, 9(1), pp.59~66 (2012), Wiley-Blackwell[DOI]
- Rachel Sensenig, "RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species", Annals of Biomedical Engineering, 39(2), pp.674~687 (2011), Springer Nature[DOI]
- Chul-Ho Kim, "Effects of atmospheric nonthermal plasma on invasion of colorectal cancer cells", Applied Physics Letters, 96(24), pp.243701~ (2010), AIP Publishing[DOI]
- Jae Won Chang, "Non-thermal atmospheric pressure plasma induces apoptosis in oral cavity squamous cell carcinoma: Involvement of DNA-damage-triggering sub-G1 arrest via the ATM", p53 pathway, 2014, pp.545, 133~140[DOI]
- Jung-Hwan Lee, "Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma", PLOS ONE, 11(2), pp.e0150279~ (2016), Public Library of Science (PLoS)[DOI]
- M. Ishaq, "Atmospheric gas plasma-induced ROS production activates TNF-ASK1 pathway for the induction of melanoma cancer cell apoptosis", Molecular Biology of the Cell, 25(9), pp.1523~1531 (2014), American Society for Cell Biology (ASCB)[DOI]
- Fumi Utsumi, "Effect of Indirect Nonequilibrium Atmospheric Pressure Plasma on Anti-Proliferative Activity against Chronic Chemo-Resistant Ovarian Cancer Cells In Vitro and In Vivo", PLoS ONE, 8(12), pp.e81576~ (2013), Public Library of Science (PLoS)[DOI]
- Nkembo AT, "Disruption of actin filaments and suppression of pancreatic cancer cell viability and migration following treatment with polyisoprenylated cysteinyl amides", Am J Cancer Res, 6, pp.2532~2546 (2016)
- Elizabeth Thomas, "A Novel Resveratrol Based Tubulin Inhibitor Induces Mitotic Arrest and Activates Apoptosis in Cancer Cells", Scientific Reports, 6(1) (2016), Springer Nature[DOI]
- 임경섭, "Resveratrol induces the apoptosis associated with activation of caspases in HTB-41 human salivary gland epidermoid carcinoma cells", Oral Biology Research, 40(1), pp.16~23 (2016), 구강생물학연구소
- Chloé Leprêtre, "Apoptosis-inducing factor (AIF) and leukocyte elastase inhibitor", l-DNase II (LEI, Apoptosis, pp.2013, 18(9)[DOI]
- Sun Ja Kim, "Cold atmospheric plasma jet-generated RONS and their selective effects on normal and carcinoma cells", Scientific Reports, 6(1) (2016), Springer Nature[DOI]