블루베리의 영양성분 및 항산화·항아세틸콜린에스터레이즈 활성
Nutritional Composition and Antioxidant·Antiacetylcholinesterase Activities of Blueberry (Vaccinium virgatum)
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초록
고부가가치 식품 산업에서 블루베리의 산업적 활용 가능성을 확인하고자 주요 영양성분 분석, invitro 항산화 활성, 그리고 아세트콜린에스터레이즈 (AChE) 효소 저해 활성을 연구하였다. 블루베리에포함된 주요 미네랄 중 칼슘이 가장 풍부한 것으로 분석되었고, 아미노산은 주로 aspartic acid와glutamic acid로 구성 되었다. 지방산의 분포는 76.78%의 포화지방산과 23.21%의 불포화 지방산으로구성되었으며, vitamin C는 22.48 mg/100 g으로 분석되었고 주요 유리당으로서는 과당과 포도당이 검출되었다. 생리적인 활성 효과를 확인하고자 블루베리의 에탄올 추출물을 염산, 에틸아세테이트, 그리고 산성메탄올을 이용하여 분획하였다. Total phenolics 함량은 각각 1.65, 2.31 그리고 4.45 mg ofGAE / g 어었으며, in vitro 항산화 효과로서의 FRAP assay와 MDA 생성 저해 효과는 안토시아닌 분획물에서 농도 의존적으로 증가하였다. 또한 에탄올 추출물, 비 안토시아닌 분획물 그리고 안토시아닌분획물에서 모두 AChE 활성을 억제하는 것으로 나타났으나, 특히 블루베리의 안토시아닌 분획물에 의해 억제되는 양상이 우수한 것으로 확인되었다. 결과적으로 다양한 영양성분을 포함하는 블루베리의 안토시아닌 분획물은 우수한 in vitro 항산화 효과와 AChE 저해 활성을 가지는 것으로 확인되었다.
Abstract
The nutritional composition, antioxidant, and anti-acetylcholinesterase (AChE) activities ofblueberry (Vaccinium virgatum) extract were investigated. A calcium was the most abundantamong minerals in blueberry. Amino acids were mainly composed of aspartic acid and glutamicacid. Distribution of fatty acids was 76.78% in saturated fatty acid and 23.21% in unsaturatedfatty acid. Vitamin C was 22.48 ㎎/100 g, and principal free sugars were fructose and glucose. Based on a bioactivity-guided isolation principle, ethanolic extracts of blueberry werefractionated to separate fractions of HCl, ethylacetate, and acidic methanol. Total phenolics were1.64, 2.31, and 4.45 ㎎ of GAE /g, respectively. Ferric reducing antioxidant power (FRAP) andmalondialdehyde (MDA) inhibitory activity of the anthocyanin fraction were increased in adose-dependent manner. In addition, we examined the effect of various fraction onacetylcholinesterase (AChE) inhibition, and our data showed that the AChE was inhibited by theanthocyanin fraction of blueberry. The results suggest that anthocyanin fraction from blueberryhas excellent antioxidant and AChE inhibitory activities.
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