본문 바로가기
KERD
논문KCI 등재

멸종위기 야생생물Ⅰ급 풍란의 효율적인 복원방법 구명

A Study on the Efficient Restoration Method of Neofinetia falcata(Thunb.) Hu. As Endangered Wild Species I Class

0

조회

0

다운로드

3

인용

0

참고문헌

키워드

초록

본 연구는 멸종위기 야생생물 Ⅰ급 풍란의 증식 및 복원을 위한 효율적인 복원을 위해 증식기술과 복원 후 생장조사를 통한 복원기술개발을 목적으로 수행되었다. 한려해상국립공원 자생지에서 채취한 씨방을 종자의 적정 생장배지 구명을 위해 4개 배지별 분석결과, 식물체 폭, 잎수, 잎길이, 뿌리수에서전체적으로 LMWP 배지에서 생장율이 가장 높게 나타났다. 증식된 270개체를 대상으로 식물체 높이,식물체 폭, 촉수, 잎길이, 잎폭, 잎수 등 6개 항목을 조사하였고, 조사시기는 이식 전, 이식복원 100일후 1차 모니터링, 이식복원 1년 후 2차모니터링 등 3회를 실시하였다. 생장량 분석결과, 복원 전 조사,사후모니터링 1차 조사의 생장량보다 사후모니터링 2차의 생장량이 증가한 것은 풍란이 동절기 후에 기온이 높아지면서 대부분의 성장이 이루어졌기 때문으로 판단된다. 단순 주 효과 분석을 실시한 결과,부착개수에 따른 생장차이는 없었으나, 부착방법에 따른 촉수와 잎수에서 수태와 돌 부착방법이 유의수준으로 나타났다. 로지스틱 회귀분석으로 생존율의 영향인자를 분석한 결과, 생존확률에 영향을 미치는인자는 나무부착 여부가 부정적인 인자로 영향력이 가장 크게 나타났으며, 잎수가 적을수록 생존확률이높게 나타났다. 향후, 미기후 환경인자와 생존율, 부착방법과 부착개수에 따른 생장율 관계 구명을 통한 다각적인 분석이 추가적으로 필요할 것으로 판단된다.

Abstract

This study was conducted for the purpose of restoration methods development through theinvestigation after the growth and proliferation techniques to restore effective for restoringgrowth and restoration of endangered wildlife ClassⅠ Neofinetia falcata. We collected fruits in Hallyeohaesang National Park for proliferation. As a result of ananalysis of four culture medium to search for reasonable culture medium, LMWP, MS,MS+Charcoal and Hyponex that generally used in orchid growth, The width of the plant, thenumber of leaves, the length of the leave and the number of roots, over all, the growth ratewere highest in the LMWP. We probed 270 individuals that were restored about six items thatincluding the height of the plant, the width of the plant, the number of shoots, the length ofthe leaf, the width of the leaf and the number of leaves. And we investigated three times at before(implantation)restoration, 100 days after the restoration and one year after the restoration. In the analysis of growth amount after the restoration, Generally Neofinetia falcata grew up atspring that temperature increased. So, at the 2nd monitoring, growth amount increased than thesurvey before restoration and the 1st monitoring. As a result of a simple main effect analysis,there was no difference in growth according to the number of attachments, but in the numberof shoots and leaves, there were significant differences in attach to sphagnum moss and stone. An a result of logistic regression analysis about the factors that affecting the survival rate,method that attached to tree was the biggest negative factor of survival, on the other handsurvival rate was higher in the less leaves. Additionally, we need more studies aboutrelationships of growth rate according to micro-climate environmental factors, survival rate,method of attachment and the number of attachments.

참고문헌 (14)

  1. Chung JD, "Seed culture of Neofinetia falcata in vitro: Basic study on asymbiotic germination of seed and gowth of seedlings", Kor. J. plant biotechnology, 1, pp.49~66 (1979)
  2. Chung JD, "Seed culture of Neofinetia falcata in vitro: Effect of Hyponex Medium Added with various concentrations of peptone or tryptone on asymbiotic germination of seeds and growth of seedlings", Kor. J. plant biotechnology, 7(1), pp.13~22 (1980)
  3. Ham SH, "The multiple propagation of mericlones from shoot tip culture of Neofinetia falcata", pp.2002
  4. 지선옥, "목초액이 기내 배양한 풍란(Neofinetia falcata)의 내생물질 함량 및 활성에 미치는 영향", 생명과학회지, 15(4), pp.673~677 (2005), 한국생명과학회
  5. Kano K, "Studies on the media for orchid seed germination", Memories Fac Agric Kagawa Univ, 20, pp.1~68 (1965)
  6. Kim HG, "Studies on asymbiotic germination of Bletilla striata, Neofinetia falcata and Aerides japonicum seeds", pp.1999
  7. kim HG, "Studies on asymbiotic germination of Bletilla striata, Neofinetia falcata and Aerides japonicum seeds", J. subtropical agriculture and biotechnology, 16, pp.145~157 (1999)
  8. 김영기, "엽록체 matK 와 핵 ITS 염기서열을 이용한 나도풍란속 및 풍란속의 계통과 종동정", 식물분류학회지, 44(1), pp.39~50 (2014), 한국식물분류학회
  9. Species Restoration Technol. Institute plants restoration center, research performance report(2011~2014), "Species Restoration Technol. Institute", 31~41 (2014)
  10. Lee TB, "Colouerd flora of Korea", pp.780~ (2003), Hyangmunsa publishing company
  11. Hu)의 계통분류학적 연구, "석사", 대전대학교 대학원, 2006
  12. Lloyd G, "Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture", Proc. International Plant Propagation Sci, 30, pp.421~427 (1981)
  13. Murashigie T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Plant Physiol, 15, pp.473~497 (1962)
  14. Soonchunhyang, Daejeon University, Ministry of Environment, "Shin HC", Conservation of an endangered Neofinetia falcata in and ex situ and development of cooperative model within local working group, 21~87 (2006)

관련 논문AI추천

이 간행물의 다른 논문 (22건)